S100-glia regulation of GABA transport across the nerve cell membrane

Hydén, Holger; Lange, Paul W.; Larsson, Sune

doi:10.1016/0022-510x(80)90173-2

Cited by 46 publications

(12 citation statements)

References 18 publications

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“…This swelling activates locally ‘maxi’ chloride channels across which synaptic cleft GABA permeates, thus clearing the synaptic space. Although actually there is GABA within the Deiters’ neurones (Okada & Shimada 1976), at these synaptic sites a counter‐flow of the neurotransmitter is unlikely because GABA permeability is much greater in the out → in than in the in → out direction (Hydén et al . 1980).…”

Section: Discussionmentioning

confidence: 99%

“…Single big Deiters’ neurones were rapidly isolated from the rabbit lateral vestibular nucleus and their plasma membranes microdissected by microtools, according to Hydén’s technique (Hydén 1959, Hydén et al . 1980).…”

Section: Methodsmentioning

confidence: 99%

“…1980). Each membrane was then put on a thin glass over its central 30 μ m ∅ hole (Hydén et al . 1980).…”

Section: Methodsmentioning

confidence: 99%

“…Actually, previous studies on single plasma membranes from rabbit Deiters’ neurones by the microchamber device built by Hydén’s group (Hydén et al . 1980), suggested that GABA can diffuse across the Deiters’ membrane (Hydén & Cupello 1987).…”

mentioning

confidence: 99%

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GABA and chloride permeate via the same channels across single plasma membranes microdissected from rabbit Deiters’ vestibular neurones

Rapallino¹,

Cupello²

2001

Acta Physiologica Scandinavica

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The permeation of labelled gamma-aminobutyric acid (GABA) across single microdissected Deiters' membranes has been studied in a microchamber system. The GABA permeation is via pores which are blocked by 4,4'-diisothiocyanato stilbene-2-2'disulphonic acid (DIDS). As this substance blocks as well chloride permeation across these membranes we tested whether GABA and chloride permeate across the same pores. Membrane pre-treatment with different doses of corticotropin releasing factor (CRF), a membrane permeant cyclic AMP analogue and phalloidin parallelly block the permeation of the two substances. Thus, it is most probable that GABA and chloride pass across the same pores. These pores may be swelling activated ones, opened by the mechanical stress on the membranes in the microchamber system. The passage of GABA across these pores may be of physiological importance in the termination of GABA inhibitory action on the vestibular Deiters' neurones.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…1980). Each membrane was then put on a thin glass over its central 30 μ m ∅ hole (Hydén et al . 1980).…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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GABA and chloride permeate via the same channels across single plasma membranes microdissected from rabbit Deiters’ vestibular neurones

Rapallino¹,

Cupello²

2001

Acta Physiologica Scandinavica

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“…Its expression in C6 cultures is increased by the formation of cell-to-cell contacts at the beginning of confluency (Labourdette and Mandel, 1978), and by treating the cells with norepinephrine, theophylline, IBMX, dibutyryl cyclic AMP (Labourdette and Mandel, 19801, or forskolin (Higashida et al, 1985).The specific role of S-100 protein within these cells is still largely unknown. It has been suggested to be a calcium-dependent modulator of protein phosphorylation (Patel et al, 1983;Qi and Kuo, 1984) and microtubule assembly (Baudier et al, 19821, but other functions have been ascribed to it (Hyden and Lange, 1981;Hyden et al, 1980).…”

mentioning

confidence: 99%

Effect of sodium butyrate on S‐100 protein levels and the cAMP response

Hirschfeld

Bressler

1987

Journal Cellular Physiology

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Sodium butyrate (NaB), when added to cell cultures, produces a variety of morphological and biochemical changes. We examined its effects, in nM concentrations, on the expression of two glioma cell-associated proteins, glial fibrillary acidic protein (GFAP) and S-100 protein in human glioma-derived cell line (RF), and of S-100 protein in the C6 rat glioma cell line. GFAP levels decreased by about 50% in the RF cell line, and S-100 protein levels decreased protein levels decreased by about 40% after treatment with 1 mM NaB for 48 h. In the C6 rat glioma cell line, isoproterenol with theophylline was found to increase S-100 levels by two-fold over basal levels. NaB was found to inhibit the induction of S-100 protein but exhibited no effect on the basal levels of the protein. Other short chain fatty acids, including sodium propionate and sodium isobutyrate, exhibited partial inhibitory activity. NaB, at an EC50 of 1 mM, was also found to inhibit both the beta-adrenergic and the forskolin-mediated increase in cAMP levels in these cells. This suggests that NaB may inhibit cells from expressing S-100 protein by attenuating cAMP levels.

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