S100-like protein in the goldfish ( Carassius auratus ) kidney

By means of immunochemistry and immunohistochemistry, we investigated in the kidney of freshwater and marine teleostean species for the presence and localization of three neurotrophins: nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin (NT)-3. In both species studied, NGF-like and NT-3-like immunoreactivity were present in the kidney with different distribution patterns, while BDNF-like immunoreactivity was never detected. In goldfish, NGF-like and NT-3-like immunoreactivity were identified extensively in cells along part of the arterial branches adjacent to the afferent arterioles. In scorpion fish, NGF-like and NT-3-like immunoreactive cells were observed both on afferent arterioles and on adjacent secondary branches derived from renal arteries. No immunoreactivity was detected in other renal structures. A staining pattern of immunoreactivity similar to that obtained for NGF and NT-3 was detected utilizing S100 antibody as a juxtaglomerular (JG) cell marker. Double immunolabellings NGF/S100 and NT-3/S100 evidenced the coexistence of neurotrophin-like proteins and S100-like protein in the same immunoreactive cells, thus identifying them as juxtaglomerular cells. Western blot analysis revealed the presence of molecules immunoreactive to NGF and NT-3, whose molecular weights were very similar to those of the corresponding mammalian neurotrophins. These findings extend the presence and distribution of NGF-like and NT-3-like IR in the kidney to teleost species, suggesting a probable participation of these proteins in the renal functions of freshwater and marine teleosts.

Section: Discussionsupporting

confidence: 95%

Section: Double Immunofluorescence and Confocal Laser Scanning Microsmentioning

confidence: 97%

See 1 more Smart Citation

Distribution of NGF and NT‐3‐like protein immunoreactivity in the teleost kidney

Arcamone

Lucini

Borzacchiello

et al. 2005

“…In a previous study, we observed that in the kidney of goldfish, S100-like immunoreactivity was located in JG cells distributed on the arteries but not on the afferent arterioles (de Girolamo et al, 2001). This distribution pattern resembles the one observed in channa kidney and differentiates from the ones of others saltwater fish kidney studied.…”

Section: Discussionmentioning

confidence: 95%

“…S100␣ (S100A1) and S100␤ (S100B) were localized abundantly in mammalian renal cells (Haimoto et al, 1987;Molin et al, 1984), confirming the role of these proteins in the regulation of pH, water, and electrolytes. Recently, we have described for the first time the presence and distribution of S100-like protein in the kidney of a freshwater fish (Carassius auratus) (de Girolamo et al, 2001). S100-like immunoreactivity was detected only in juxtaglomerular cells, and no immunoreactivity was observed in other tracts of the goldfish kidney.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical localization of S100‐like protein in non‐mammalian kidney

Girolamo¹,

Arcamone²,

Pelagalli³

et al. 2003

The immunolocalization of S100-like protein was investigated in the kidney of saltwater fishes (Dicentrarchus labrax; Coris julis; Serranus cabrilla; Scorpaena porcus), amphibia (Rana aesculenta), reptiles (Lacerta viridis), and aves (Gallus domesticus; Strutio camelus). S100-like immunoreactivity was detected in the juxtaglomerular cells of all saltwater fishes studied. No immunoreactivity was observed in other tracts of the nephron or in the interstitial tissue. In frog kidney, S100-like immunoreactive cells were localized in the proximal tubule, singly distributed or placed side by side in clusters of two or three cells. S100-like immunoreactive cells were distributed in the distal and in the collecting tubules in lizard, chicken, and ostrich kidney. In the distal tubule of lizard kidney, S100-like immunoreactive cells were numerous and uniformly distributed. In lizard collecting tubules, S100-like immunoreactive cells showed less intense immunoreactivity than in the distal tubule, except for a cluster of cells at the junction with the initial collecting duct. In chicken and ostrich kidney, S100-like immunoreactive cells of the distal tubules were closely packed together. In the collecting tubules, S100-like immunoreactive cells were alternate to negative cells. These results indicate the high conservation degree of S100 proteins through phylogenesis and suggest a functional role for these proteins in the vertebrate kidney.

Expression and distribution of S100 protein in the nervous system of the adult zebrafish (Danio rerio)

Germanà

Marino

Guerrera

et al. 2007

Self Cite

S100 proteins are EF-hand calcium-binding protein highly preserved during evolution present in both neuronal and non-neuronal tissues of the higher vertebrates. Data about the expression of S100 protein in fishes are scarce, and no data are available on zebrafish, a common model used in biology to study development but also human diseases. In this study, we have investigated the expression of S100 protein in the central nervous system of adult zebrafish using PCR, Western blot, and immunohistochemistry. The central nervous system of the adult zebrafish express S100 protein mRNA, and contain a protein of approximately 10 kDa identified as S100 protein. S100 protein immunoreactivity was detected widespread distributed in the central nervous system, labeling the cytoplasm of both neuronal and non-neuronal cells. In fact, S100 protein immunoreactivity was primarily found in glial and ependymal cells, whereas the only neurons displaying S100 immunoreactivity were the Purkinje's neurons of the cerebellar cortex and those forming the deep cerebellar nuclei. Outside the central nervous system, S100 protein immunoreactivity was observed in a subpopulation of sensory and sympathetic neurons, and it was absent from the enteric nervous system. The functional role of S100 protein in both neurons and non-neuronal cells of the zebrafish central nervous system remains to be elucidated, but present results might serve as baseline for future experimental studies using this teleost as a model.