Rural population of Ukraine mainly consume water from wells and individual wells, which (in the vast majority) are in unsatisfactory technical and sanitary condition. For the estimation of the quality of the spring waters in some settlements of Mostyska district, the seasonal samples of were taken in Mostyska, Malniv, Gusiakiv, Pidhat, Volytsia, Nagirne, and Krukenychi. Biotesting of well water was carried out, water was analyzed according to physicochemical and bacteriological indicators. It was established that toxicity is higher than average in the villages Krukenychi, Gusiakiv, Volytsia and Malniv, and in Mostyska high toxicity. The content of nitrites and phosphates did not exceed the MPC in all investigated waters. The content of nitrates and ammonia in the spring waters of the investigated area exceeded the MPC in most settlements, with the exception of samples from the villages Pidhat and Nagirne. In addition, only spring water from the villages Nagirne and Pidhat meets the bacteriological indicators for the quality of drinking water.