Saccadic premotor burst neurons and histochemical correlates of their firing patterns in rhesus monkey

Mayadali, Ümit Suat; Lienbacher, Karoline; Shaikh, Aasef G.; Horn, Anja K.E.

doi:10.1016/j.jns.2022.120328

Cited by 2 publications

(7 citation statements)

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“…However, steep differences in HCN1 immunoreactivity for INTs in both trochlear and abducens nuclei was unexpected. Moreover, SIF motoneurons in human abducens also showed some variety in the immunolabeling intensity (Figure 6f), which was not observed in monkey abducens nucleus (Mayadali et al, 2022). This might indicate that SIF motoneurons could be regulating a range of post-inhibitory readiness for action potential, and resting membrane potential through HCN1 expression (Kase and Imoto, 2012).…”

Section: Internuclear Neurons Of the Human Abducens Nucleus Lack Hcn1...mentioning

confidence: 90%

“…One striking difference between monkey and human INTs was found in HCN1 immunolabeling. In monkey abducens nucleus, INTs were found strongly immunolabeled for HCN1 similar to SIF motoneurons, whereas MIF motoneurons lacked HCN1 labeling ( Mayadali et al, 2022 ). In human abducens nucleus, however, while SIF motoneurons mostly expressed strong HCN1 immunolabeling, INTs exhibited no immunoreactivity ( Figures 6e, f ).…”

Section: Discussionmentioning

confidence: 99%

“…As extensively discussed in our previous work (Horn et al, 2018;Mayadali et al, 2021Mayadali et al, , 2022, PN-ensheathment density has direct and indirect effects on firing characteristics of neurons through (co)regulating transmitter and ion channel content of neuronal membranes, as well as affecting calcium-binding protein expression of neurons. In this context, the variety of PN expression in human ocular motonuclei could be interpreted as a contribution to a wide range of variety in biophysiological features of motoneurons to generate complex eye movements.…”

Section: Biophysiological Implications Of the Varied Perineuronal Net...mentioning

confidence: 92%

“…In the monkey abducens and trochlear nuclei, significant differences in immunolabeling density were found between MIF and SIF motoneurons with respect to Kv1.1, Kv3.1, HCN1 and Cav3.1 channels (Mayadali et al, 2021(Mayadali et al, , 2022. Despite the fact that these channels generally exhibited somewhat uniform immunolabeling density where they were immunopositive, Kv1.1 channel showed great variability in terms of immunolabeling density (Mayadali et al, 2021).…”

Section: Variable Immunolabeling Of Ion Channel Proteins In Mif and S...mentioning

confidence: 98%

“…Low voltage activated cation channels were another group of ion channels that showed differences in immunolabeling of MIF and SIF motoneurons in monkey trochlear and abducens nuclei. Firstly, HCN1 channel subunit was absent in MIF motoneurons, whereas SIF motoneurons exhibited strong membrane-labeling ( Mayadali et al, 2022 ). Moreover, T-type calcium channel subunit Cav3.1 was prominent in MIF motoneurons, in contrast to only few SIF motoneurons having weak Cav3.1 labeling ( Mayadali et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Ion channel profiles of extraocular motoneurons and internuclear neurons in human abducens and trochlear nuclei

Mayadali,

Chertes,

Sinicina

et al. 2024

Front. Neuroanat.

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IntroductionExtraocular muscles are innervated by two anatomically and histochemically distinct motoneuron populations: motoneurons of multiply-innervated fibers (MIF), and of singly-innervated fibers (SIF). Recently, it has been established by our research group that these motoneuron types of monkey abducens and trochlear nuclei express distinct ion channel profiles: SIF motoneurons, as well as abducens internuclear neurons (INT), express strong Kv1.1 and Kv3.1b immunoreactivity, indicating their fast-firing capacity, whereas MIF motoneurons do not. Moreover, low voltage activated cation channels, such as Cav3.1 and HCN1 showed differences between MIF and SIF motoneurons, indicating distinct post-inhibitory rebound characteristics. However, the ion channel profiles of MIF and SIF motoneurons have not been established in human brainstem tissue.MethodsTherefore, we used immunohistochemical methods with antibodies against Kv, Cav3 and HCN channels to (1) examine the human trochlear nucleus in terms of anatomical organization of MIF and SIF motoneurons, (2) examine immunolabeling patterns of ion channel proteins in the distinct motoneurons populations in the trochlear and abducens nuclei.ResultsIn the examination of the trochlear nucleus, a third motoneuron subgroup was consistently encountered with weak perineuronal nets (PN). The neurons of this subgroup had -on average- larger diameters than MIF motoneurons, and smaller diameters than SIF motoneurons, and PN expression strength correlated with neuronal size. Immunolabeling of various ion channels revealed that, in general, human MIF and SIF motoneurons did not differ consistently, as opposed to the findings in monkey trochlear and abducens nuclei. Kv1.1, Kv3.1b and HCN channels were found on both MIF and SIF motoneurons and the immunolabeling density varied for multiple ion channels. On the other hand, significant differences between SIF motoneurons and INTs were found in terms of HCN1 immunoreactivity.DiscussionThese results indicated that motoneurons may be more variable in human in terms of histochemical and biophysiological characteristics, than previously thought. This study therefore establishes grounds for any histochemical examination of motor nuclei controlling extraocular muscles in eye movement related pathologies in the human brainstem.

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Section: Internuclear Neurons Of the Human Abducens Nucleus Lack Hcn1...mentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Biophysiological Implications Of the Varied Perineuronal Net...mentioning

confidence: 92%

Section: Variable Immunolabeling Of Ion Channel Proteins In Mif and S...mentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Ion channel profiles of extraocular motoneurons and internuclear neurons in human abducens and trochlear nuclei

Mayadali,

Chertes,

Sinicina

et al. 2024

Front. Neuroanat.

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A Cognitive-Motor Dissociation of NMDAR Blockade in Behaving Non-Human Primates

Pouget

Paré

Daye

2023

Preprint

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Ketamine is widely used to probe cognitive functions relying on the properties of NMDAR synaptic transmission. Numerous works have already proved that cognitive performance and adjustments in the decision or perceptual domains are affected after ketamine injection in the general circulation. Here, we take advantage of that in the brain stem; horizontal saccade deceleration is controlled by glycine NMDA-gated current. In contrast, GABA current controls vertical deceleration to demonstrate that despite general circulation level manipulation of NMDAR synaptic transmission, the skewness of the saccade appeared to be in the motor brainstem generator circuit differentially maintained. The deacceleration of the saccade elicited toward a horizontal target was substantially decreased, while the deacceleration of a vertical saccade remained largely unaffected. These results provide a functional marker for estimating NMDA-gated specificity acting in the pre-frontal cortex while maintaining its specificity among the GABA circuit of drugs in general circulation.

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Saccadic premotor burst neurons and histochemical correlates of their firing patterns in rhesus monkey

Cited by 2 publications

References 75 publications

Ion channel profiles of extraocular motoneurons and internuclear neurons in human abducens and trochlear nuclei

Ion channel profiles of extraocular motoneurons and internuclear neurons in human abducens and trochlear nuclei

A Cognitive-Motor Dissociation of NMDAR Blockade in Behaving Non-Human Primates

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