Saccharibacillus deserti sp. nov., isolated from desert soil

Abstract

A Gram-stain-positive, facultatively anaerobic bacterial strain, designated WLJ055 T , with polar and subpolar flagella was isolated from the top layer of desert soil from Erdos, Inner Mongolia, northern China. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain WLJ055T was a member of the genus Saccharibacillus, and shared 97. During an analysis of the bacterial diversity in the Kubuqi desert, a bacterial strain, designated WLJ055 T , was isolated from the top layer of soil (0-15 cm) collected from the Kubuqi desert (408 189 N 1098 289 E), Ordors, Inner Mongolia, northern China, by using the 10-fold dilution plating technique on Luria-Bertani (LB) agar (g l 21 : tryptone 10.0, yeast extract 5.0, NaCl 10.0, and agar 20.0; pH 7.0). The isolate was cultivated at 30 8C under aerobic conditions and purified by repeatedly streaking on LB agar. Biomass for molecular systematic and chemotaxonomic analyses was prepared by culturing strain WLJ055 T in liquid LB medium at 30 8C for two days and harvesting the cells by centrifugation.The genomic DNA was extracted by using a DNA extraction kit (BioTech) according to the manufacturer's instructions. The 16S rRNA gene was amplified with the universal primer set, 27F (59-AGA GTT TGA TCM TGG CTC AG-39) and 1492R (59-TAC GG(C/T) TAC CTT GTT ACG ACT T-39) by using the protocol described earlier (Wang et al., 2007). The PCR amplicon was purified with a PCR purification kit (Biotech) and sequenced after the resulting DNA fragments were ligated to a pMD19-T vector (TaKaRa) according to the manufacturer's instructions. The nearly complete (1380 bp) sequence was compared with the available 16S rRNA genes of strains of validly published species using the BLAST program (http:// www.ncbi.nlm.nih.gov/blast) to determine its phylogenetic affiliation. Results of BLAST searches indicated that the sequence shared a relatively high level of similarity (approximate 97.2 %) with sequences of species of the genus Saccharibacillus. In order to elucidate the phylogenetic position of strain WLJ055 T , the 16S rRNA gene sequences for type strains of species of the genus Saccharibacillus as well as of the related genus, Paenibacillus were downloaded. Sequences were aligned using CLUSTAL X software (Thompson et al., 1997). Phylogenetic trees were 3These authors contributed equally to this work.

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“…This species was isolated from ginseng soils (Choi et al 2016). Additional genera were detected in S1, such as Saccharibacillus, with the species Saccharibacillus deserti, which was isolated from desert soil in China (Sun et al 2016).…”

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“…The genus Saccharibacillus belongs to the family Paenibacillaceae and is mainly isolated as plant endophytes and from the phyllosphere from plants such as cotton, wheat, vegetables and fruits, sugar cane, and barley seed. Furthermore, this genus can also be found in desert soil and mine tailings (Rivas et al 2008 ; Yang et al 2009 ; Han et al 2016 ; Kampfer et al 2016 ; Sun et al 2016 ; Besaury and Remond 2020 ; Jiang et al 2020 ; Darji et al 2021 ). Plant-associated bacteria, especially plant endophytes, play an important role in PGP and productivity (Santoyo et al 2016 ; Hardoim et al 2008 ; Singh et al 2021 ; Chen et al 2019 ).…”

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