Saccharification Yield through Enzymatic Hydrolysis of the Steam-Exploded Pinewood

Abstract

Pressure, temperature, and retention time are the most studied parameters in steam explosion pretreatment. However, this work aimed to fix these parameters and to evaluate the influences of several less investigated steam explosion parameters on the saccharification yield in hydrolysis. In this study, firstly, pinewood samples smaller than 200 µm were treated with steam explosion at 190 °C for 10 min. The variable parameters were biomass loading, N2 pressure, and release time. Steam-exploded samples were hydrolyzed with the Trichoderma reesei enzyme for saccharification for 72 h. The sugar content of the resultant products was analyzed to estimate the yield of sugars (such as glucose, xylose, galactose, mannose, and arabinose). The best glucose yield in the pulp was achieved with 4 g of sample, N2 pressure of 0.44 MPa, and short release time (22 s). These conditions gave a glucose yield of 97.72% in the pulp, and the xylose, mannose, galactose, and arabinose yields in the liquid fraction were found to be 85.59%, 87.76%, 86.43%, and 90.3%, respectively.

Development of an ammonia pretreatment that creates synergies between biorefineries and advanced biomass logistics models

“…StEx has been very well studied for SCB in previous reports and has been selected to benchmark acidic pretreatments. 40,46,47 Fig. 3 shows that the latest generation of ammonia pretreatment technologies, such as COBRA, COBRA-LE and EA release significantly higher fermentable sugar levels relative to AFEX and StEx for most operational conditions studied herein.…”

Structural Modification of Empty and Partially Filled Paddy Grain Through Hydrothermal Pre‐treatment as a Novel Substrate for Fermentable Glucose Production

“…It can be postulated that the size reduction of EPFG (screen size of less than 1 mm obtained in this study) leads to an increase of surface area; providing more accessibility of biomass for degradation in a pre‐treatment process. [ 16 ] In addition, compared to the untreated G‐EPFG (Table 1), the act of grinding EPFG seems to only show favorable effects on sugar concentration when combining with mild hydrothermal pretreatment.…”

Production and detoxification of inhibitors during the destruction of lignocellulose spatial structure

“…It can damage the structure of the arrangement and degrade the hemicellulose component of lignocellulose, thus effectively improving the efficiency of enzymatic hydrolysis and enhancing the conversion efficiency (Martín-Sampedro et al 2012;Yu et al 2020;Cubas-Cano et al 2020). For example, Borand et al (2020) used pine to conduct steam explosion treatment at 190 ℃ for 10 min, and the final glucose yield was 97.7%, whereas xylose, mannose, galactose, and arabinose were 85.6%, 87.8%, 86.4%, and 90.3%, respectively. He et al (2019) used steam explosion pretreatment at a pressure of 1.5 MPa to pre-treat seabuckthorn; it was found that a reaction of 20 minutes could remove 77.2% of hemicellulose, and the sugar yield after enzymatic hydrolysis was 4.54 times that of the untreated control.…”