Saccharofermentans acetigenes gen. nov., sp. nov., an anaerobic bacterium isolated from sludge treating brewery wastewater

Chen, Shuangya; Niu, Lili; Zhang, Yongxiang

doi:10.1099/ijs.0.017590-0

Cited by 109 publications

(62 citation statements)

References 18 publications

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“…Various representative clones are found in our library, e.g., Clostridium sp. degrades cellulose, xylan, and polysaccharides (Chen et al 2010;Kato et al 2004;Zhilina et al 2005), Desulfosporosinus sp. utilizes aromatic compounds (Liu et al 2004), and Acetobacterium sp., Alkalibacter sp., and Desulfotomaculum sp.…”

Section: Discussionmentioning

confidence: 99%

“…(90% identity) which is a sulfate reducer and able to degrade o-xylene, m-xylene, and toluene (Morasch et al 2004) and from Pelotomaculum terephthalicicum (90% identity) which was isolated from methanogenic sludges as syntrophic phthalate isomer-degrading microbes (Qiu et al 2004). Both SXLL-B02 and SXLL-B18 were closely related to the 16S rRNA gene from the cultured organism Saccharofermentans acetigenes (91% Bernard et al (1978) identity), which was isolated from an upflow anaerobic sludge blanket reactor and could ferment polysaccharides (Chen et al 2010). They were also related to the anaerobic cellulolytic bacterium Clostridium straminisolvens (90% identity) ).…”

Section: Properties Of Water Producedmentioning

confidence: 99%

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Methylotrophic methanogenesis governs the biogenic coal bed methane formation in Eastern Ordos Basin, China

Guo

Liu

et al. 2012

Appl Microbiol Biotechnol

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To identify the methanogenic pathways present in a deep coal bed methane (CBM) reservoir associated with Eastern Ordos Basin in China, a series of geochemical and microbiological studies was performed using gas and water samples produced from the Liulin CBM reservoir. The composition and stable isotopic ratios of CBM implied a mixed biogenic and thermogenic origin of the methane. Archaeal 16S rRNA gene analysis revealed the dominance of the methylotrophic methanogen Methanolobus in the water produced. The high potential of methane production by methylotrophic methanogens was found in the enrichments using the water samples amended with methanol and incubated at 25 and 35°C. Methylotrophic methanogens were the dominant archaea in both enrichments as shown by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE). Bacterial 16S rRNA gene analysis revealed that fermentative, sulfate-reducing, and nitrate-reducing bacteria inhabiting the water produced were a factor in coal biodegradation to fuel methanogens. These results suggested that past and ongoing biodegradation of coal by methylotrophic methanogens and syntrophic bacteria, as well as thermogenic CBM production, contributed to the Liulin CBM reserves associated with the Eastern Ordos Basin.

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Section: Discussionmentioning

confidence: 99%

Section: Properties Of Water Producedmentioning

confidence: 99%

Methylotrophic methanogenesis governs the biogenic coal bed methane formation in Eastern Ordos Basin, China

Guo

Liu

et al. 2012

Appl Microbiol Biotechnol

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“…In order to produce H 2 S, SRB oxidize a number of endogenously and exogenously produced fermentative products. A number of phylotypes were classified in this study that identify with genera, such as Saccharofermentans [45], that are capable of fermenting numerous carbohydrates. Other organisms, belonging to genera such as Aminobacterium [46], are capable of degrading proteins.…”

Section: Discussionmentioning

confidence: 99%

Microbial community analysis of swine wastewater anaerobic lagoons by next-generation DNA sequencing

Ducey

Hunt

2013

Anaerobe

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a b s t r a c tAnaerobic lagoons are a standard practice for the treatment of swine wastewater. This practice relies heavily on microbiological processes to reduce concentrated organic material and nutrients. Despite this reliance on microbiological processes, research has only recently begun to identify and enumerate the myriad and complex interactions that occur in this microbial ecosystem. To further this line of study, we utilized a next-generation sequencing (NGS) technology to gain a deeper insight into the microbial communities along the water column of four anaerobic swine wastewater lagoons. Analysis of roughly one million 16S rDNA sequences revealed a predominance of operational taxonomic units (OTUs) classified as belonging to the phyla Firmicutes (54.1%) and Proteobacteria (15.8%). At the family level, 33 bacterial families were found in all 12 lagoon sites and accounted for between 30% and 50% of each lagoon's OTUs. Analysis by nonmetric multidimensional scaling (NMS) revealed that TKN, COD, ORP, TSS, and DO were the major environmental variables in affecting microbial community structure. Overall, 839 individual genera were classified, with 223 found in all four lagoons. An additional 321 genera were identified in sole lagoons. The top 25 genera accounted for approximately 20% of the OTUs identified in the study, and the low abundances of most of the genera suggests that most OTUs are present at low levels. Overall, these results demonstrate that anaerobic lagoons have distinct microbial communities which are strongly controlled by the environmental conditions present in each individual lagoon.Published by Elsevier Ltd.

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“…S. acetigenes produced acetate, succinate, lactate and trace amounts of H 2 from this sugar. Fumarate has been previously reported as a final product of glucose fermentation (Chen et al, 2010), but in our tests with S. acetigenes, T and neighbouring isolated strains in the Living Tree Project tree (Yarza et al, 2008). A consensus phylogenetic tree was generated using parsimony, neighbor-joining and maximum-likelihood analyses with different sets of filters, which showed stable branching.…”

Section: Differences Between Strain Zwbmentioning

confidence: 99%

Ercella succinigenes gen. nov., sp. nov., an anaerobic succinate-producing bacterium

Gelder

Sousa

Rijpstra

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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, was isolated from sludge collected from a biogas desulfurization bioreactor (Eerbeek, the Netherlands). Cells were nonspore-forming, motile, slightly curved rods (0.4-0.5 mm in diameter and 2-3 mm in length), and stained Gram-negative. The temperature range for growth was 25-40 6C, with an optimum at 37 6C. The pH range for growth was 7.0-9.0, with an optimum at pH 7.5. Strain ZWB T was able to ferment glycerol and several carbohydrates mainly to H 2 , succinate and acetate. Sulfur and fumarate could be used as electron acceptors by strain ZWB T . The G+C content of the genomic DNA was 37.6 mol%. The most abundant fatty acids were iso-C 14 : 0 and iso-C 16 : 0 DMA. On the basis of 16S rRNA gene sequence similarity, strain ZWB T belongs to the family Succinate is a valuable energy-rich compound used for organic synthesis of 1,4-butanediol, tetrahydrofuran, butyrolactone and adipic acid (McKinlay et al., 2007). Bacteria that ferment succinic acid have application possibilities, especially anaerobes that produce more than 1 mol succinate per mole of glucose. Described anaerobic succinate producers include Fibrobacter succinogenes, Anaerobiospirillum succiniciproducens, Actinobacillus succinogenes, 'Mannheimia succiniciproducens' and Basfia succiniciproducens. These micro-organisms ferment sugars, but some ferment glycerol as well. The use of glycerol as carbon source by Anaerobiospirillum succiniciproducens resulted in higher succinate production and a much higher ratio of succinate to acetate than obtained with glucose (Lee et al., 2001). Crude glycerol is an important side product of biodiesel production and therefore an interesting substrate for succinate production (Choi, 2008). Due to the massive production of biodiesel (Röttig et al., 2010;Licht, 2012), the price of bulk glycerol has dropped considerably in the past decade (Yazdani & Gonzalez, 2007;Choi, 2008). In this work, we describe a novel anaerobic succinateproducing bacterium, strain ZWB T , that can utilize glycerol and several carbohydrates as main energy sources, forming H 2 , succinate and acetate. Based on its distinctive phenotypic, genotypic and phylogenetic characteristics, strain ZWB T is proposed to represent a novel species of a new genus. Strain ZWBT was isolated from sludge collected from a biogas desulfurization bioreactor (Eerbeek, the Netherlands). Samples and dilutions thereof were inoculated in 120 ml serum vials with 50 ml of anoxic bicarbonate-buffered medium prepared as previously described (Stams et al., 1993). The basal medium was supplemented with 0.1 g yeast extract l 21, 20 mM glycerol as carbon and energy source, and 20 mM sulfate as potential electron acceptor. Routinely, cultures were incubated statically at 30 u C in the dark. When growth was detected, serial dilution series were made. The purity of the isolate was confirmed by microscopic observation. Chemical analysis of culture media pointed to fermentative growth of the isolate, and therefore sulfate was eliminated from media. In the first enrichments, 0.1 g yeast ex...

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Saccharofermentans acetigenes gen. nov., sp. nov., an anaerobic bacterium isolated from sludge treating brewery wastewater

Cited by 109 publications

References 18 publications

Methylotrophic methanogenesis governs the biogenic coal bed methane formation in Eastern Ordos Basin, China

Methylotrophic methanogenesis governs the biogenic coal bed methane formation in Eastern Ordos Basin, China

Microbial community analysis of swine wastewater anaerobic lagoons by next-generation DNA sequencing

Ercella succinigenes gen. nov., sp. nov., an anaerobic succinate-producing bacterium

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