Saccharomyces cerevisiae Fpr1 functions as a chaperone to inhibit protein aggregation

Das, Eshita; Prasad, Shivcharan; Roy, Ipsita

doi:10.1016/j.ijbiomac.2021.09.046

Cited by 7 publications

(4 citation statements)

References 76 publications

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“…In the latter case, the presence of EGFP was used to visualise protein expression by a fluorescence microscope (Nikon Eclipse, E 600). Induced cells were lysed [111], and the presence of soluble protein was confirmed by native PAGE and western blotting. Band intensities were normalised by equalising protein amounts.…”

Section: Methodsmentioning

confidence: 99%

“…The formation of oligomers by 103Q‐htt was followed by the immobilisation of cell lysates on a nitrocellulose membrane as described earlier [111]. The oligomer‐specific antibody A11 (AHB0052; Invitrogen Biosciences India Pvt.…”

Section: Methodsmentioning

confidence: 99%

“…Aggregation of 103Q‐htt was monitored by filter retardation assay on a cellulose acetate membrane as described earlier [111]. The membrane was probed with polyglutamine antibody (cat.…”

Section: Methodsmentioning

confidence: 99%

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The functional role of Ire1 in regulating autophagy and proteasomal degradation under prolonged proteotoxic stress

2023

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Inhibition of endoribonuclease/kinase Ire1 has shown beneficial effects in many proteotoxicity‐induced pathology models. The mechanism by which this occurs has not been elucidated completely. Using a proteotoxic yeast model of Huntington's disease, we show that the deletion of Ire1 led to lower protein aggregation at longer time points. The rate of protein degradation was higher in ΔIre1 cells. We monitored the two major protein degradation mechanisms in the cell. The increase in expression of Rpn4, coding for the transcription factor controlling proteasome biogenesis, was higher in ΔIre1 cells. The chymotrypsin‐like proteasomal activity was also significantly enhanced in these cells at later time points of aggregation. The gene and protein expression levels of the autophagy gene Atg8 were higher in ΔIre1 than in wild‐type cells. Significant increase in autophagy flux was also seen in ΔIre1 cells at later time points of aggregation. The results suggest that the deletion of Ire1 activates UPR‐independent arms of the proteostasis network, especially under conditions of aggravated stress. Thus, the inhibition of Ire1 may regulate UPR‐independent cellular stress‐response pathways under prolonged stress.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The functional role of Ire1 in regulating autophagy and proteasomal degradation under prolonged proteotoxic stress

2023

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“… 19 Recently, budding yeast FKBP12 was shown to suppress protein aggregation in vitro , indicating a chaperon function. 20 In the fission yeast Schizosaccharomyces pombe , physiological roles of FKBP12, encoded by the fkh1 gene, have not been reported, although the effects of rapamycin occur through FKBP12 21 and FK506 phenocopies mutants lacking the calcineurin catalytic subunit, Ppb1. 22 , 23 …”

Section: Introductionmentioning

confidence: 99%

FK506-binding protein, FKBP12, promotes serine utilization and negatively regulates threonine deaminase in fission yeast

Sasaki¹,

Nishimura²,

Yashiroda³

et al. 2022

iScience

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Climate‐Resilient Crops for CO₂Rich‐Warmer Environment

Kundu

Das

Singh

et al. 2023

Global Climate Change and Plant Stress Management

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Saccharomyces cerevisiae Fpr1 functions as a chaperone to inhibit protein aggregation

Cited by 7 publications

References 76 publications

The functional role of Ire1 in regulating autophagy and proteasomal degradation under prolonged proteotoxic stress

The functional role of Ire1 in regulating autophagy and proteasomal degradation under prolonged proteotoxic stress

FK506-binding protein, FKBP12, promotes serine utilization and negatively regulates threonine deaminase in fission yeast

Climate‐Resilient Crops for CO₂Rich‐Warmer Environment

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Saccharomyces cerevisiae Fpr1 functions as a chaperone to inhibit protein aggregation

Cited by 7 publications

References 76 publications

The functional role of Ire1 in regulating autophagy and proteasomal degradation under prolonged proteotoxic stress

The functional role of Ire1 in regulating autophagy and proteasomal degradation under prolonged proteotoxic stress

FK506-binding protein, FKBP12, promotes serine utilization and negatively regulates threonine deaminase in fission yeast

Climate‐Resilient Crops for CO2Rich‐Warmer Environment

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Climate‐Resilient Crops for CO₂Rich‐Warmer Environment