Sacrificial BSA to block non‐specific adsorption on organosilane adlayers in ultra‐high frequency acoustic wave sensing

Sheikh, Sonia; Blaszykowski, Christophe; Thompson, Michael

doi:10.1002/sia.5322

Cited by 16 publications

(17 citation statements)

References 25 publications

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“…The coil and piezoelectric substrate are located in a sample cell, wherein a running buffer is flowed, hence the EMPAS operates in an online and real-time detection format. In practice, the sensor has been successfully applied for the detection of proteins (Sheikh et al, 2011(Sheikh et al, , 2010, and lipopolysaccharide pathogens (Thompson et al, 2015), as well as for the evaluation of surface fouling (Sheikh et al, 2013. Functionalization of the EMPAS sensor platforms is generally achieved through the use of conditioning organic adlayers.…”

Section: Introductionmentioning

confidence: 99%

Ultra-high frequency piezoelectric aptasensor for the label-free detection of cocaine

Neves

Blaszykowski²,

Bokhari

et al. 2015

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

Ultra-high frequency piezoelectric aptasensor for the label-free detection of cocaine

Neves

Blaszykowski²,

Bokhari

et al. 2015

Biosensors and Bioelectronics

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“…Unless otherwise specified, all materials were purchased from Sigma Aldrich. MEG-Cl and PFP-TTTA were synthesized according to previously published methods [46,47]. Goat serum was used for testing and was purchased from Sigma Aldrich.…”

Section: Methodsmentioning

confidence: 99%

“…In order to develop a test for LPA using these proteins, the molecules must first be bound to a surface. In this work, we employ a trichlorosilane surface linker such as 3-(3-(trichlorosilyl)propoxy)propanoyl chloride (MEG-Cl) [46], or perfluorophenyl 12-(trichlorosilyl)dodecanoate (PFP-TTTA) [47] (Figure 2). These linkers can be bound down to a surface via their trichlorosilane functionality, and extended with N α ,N α -bis(carboxymethyl)-l-lysine, which can then be employed for binding the polyhistidine-tagged protein form of gelsolin bound to dye modified actin [48].…”

Section: Introductionmentioning

confidence: 99%

Detection of the Ovarian Cancer Biomarker Lysophosphatidic Acid in Serum

Franier

Thompson

2020

Biosensors

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Lysophosphatidic acid (LPA) is present during the medical condition of ovarian cancer at all stages of the disease, and, therefore possesses considerable potential as a biomarker for screening its presence in female patients. Unfortunately, there is currently no clinically employable assay for this biomarker. In the present work, we introduce a test based on the duel protein system of actin and gelsolin that could allow the quantitative measurement of LPA in serum samples in a biosensing format. In order to evaluate this possibility, actin protein was dye-modified and complexed with gelsolin protein, followed by surface deposition onto silica nanoparticles. This solid-phase system was exposed to serum samples containing various concentrations of LPA and analyzed by fluorescence microscopy. Measurements conducted for the LPA-containing serum samples were higher after exposure to the developed test than samples without LPA. Early results suggest a limit of detection of 5 µM LPA in serum. The eventual goal is to employ the chemistry described here in a biosensor configuration for the large population-scale, rapid screening of women for the potential occurrence of ovarian cancer. these elevated levels indicate that LPA is a very promising marker for the early-stage detection of ovarian cancer.Commonly, LPA is detected and quantified in samples using standard analytical methods. This includes the use of capillary electrophoresis and ultraviolet detection [17,18], gas chromatography paired with flame atomic absorption spectroscopy or mass spectrometry [13,[19][20][21], thin-layer chromatography paired with mass spectrometry [15,[22][23][24][25], liquid chromatography paired with mass spectrometry [14,[26][27][28][29][30] or absorbance spectroscopy [31] and matrix-assisted laser desorption/ionization mass spectrometry [32][33][34]. Although these techniques are very sensitive towards LPA, they almost always require lipid extraction or other work-up of the serum samples before use. They also are highly labor intensive, time consuming, and use highly sophisticated instrumentation to quantify the marker. As such, these technologies are inappropriate for practical clinical analysis and, therefore, new methods that use simpler protocols and that are rapid and easy to perform in serum are required for an LPA assay to be included in a large-scale screening protocol for ovarian cancer.The protein gelsolin [35], which binds to LPA through a small chain of amino acids known as the PIP 2 -binding domain, is eminently capable of acting as a selective probe for the marker [36]. Gelsolin binds LPA with a high affinity measured by its K d of 6 nM [35,37]. The PIP 2 -binding domain, however, only binds LPA with a K d of 920 nM, suggesting that the interactions of LPA and gelsolin are heavily dependent on other components of the protein. The molecule itself is a large six-domain protein, with a molecular mass over 80 kDa [35]. Of the six domains, the protein essentially exists as two identical components comprised of domains 1-3 and dom...

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“…The EMPAS working frequency is typically 0.94 GHz, representing a 47-harmonic frequency. It is possible to even reach a working frequency of 1.06 GHz, corresponding to a 53-harmonic frequency [ 53 ]. The electromagnetic excitation mechanism produces an oscillation without gold electrodes at the crystal surface.…”

Section: Surface Acoustic Methodsmentioning

confidence: 99%

Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods

Dizon

Tatarko

Hianik

2020

Sensors

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This review is focused on the application of surface and volume-sensitive acoustic methods for the detection of milk proteases such as trypsin and plasmin. While trypsin is an important protein of human milk, plasmin is a protease that plays an important role in the quality of bovine, sheep and goat milks. The increased activity of plasmin can cause an extensive cleavage of β-casein and, thus, affect the milk gelation and taste. The basic principles of surface-sensitive acoustic methods, as well as high-resolution ultrasonic spectroscopy (HR-US), are presented. The current state-of-the-art examples of the application of acoustic sensors for protease detection in real time are discussed. The application of the HR-US method for studying the kinetics of the enzyme reaction is demonstrated. The sensitivity of the acoustics biosensors and HR-US methods for protease detection are compared.

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Sacrificial BSA to block non‐specific adsorption on organosilane adlayers in ultra‐high frequency acoustic wave sensing

Cited by 16 publications

References 25 publications

Ultra-high frequency piezoelectric aptasensor for the label-free detection of cocaine

Ultra-high frequency piezoelectric aptasensor for the label-free detection of cocaine

Detection of the Ovarian Cancer Biomarker Lysophosphatidic Acid in Serum

Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods

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