Safest level of tricaine methanesulfonate (ms-222) to induce anesthesia in juveniles of Matrinxã, Brycon cephalus

Roubach, Rodrigo; Gomes, Levy de Carvalho; Val, Adalberto Luís

doi:10.1590/1809-43922001311163

Cited by 34 publications

(23 citation statements)

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“…similar resting levels have also been reported in other studies in which matrinxã has been exposed to different stressors (Avilez et al, 2004;roubach et al, 2001). Band intensity of Gill HSP70 in juvenile matrinxã subjected to a 1h-cold shock (from 28°C to 18°C).…”

Section: Discussionsupporting

confidence: 85%

Physiological stress responses in the warm-water fish matrinxã (Brycon amazonicus ) subjected to a sudden cold shock

et al. 2008

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The present work evaluated several aspects of the generalized stress response [endocrine (cortisol), metabolic (glucose), hematologic (hematocrit and hemoglobin) and cellular (HsP70)] in the Amazonian warm-water fish matrinxã (Brycon amazonicus) subjected to an acute cold shock. This species farming has been done in south America, and growth and feed conversion rates have been interesting. However, in subtropical areas of Brazil, where the water temperature can rapidly change, high rates of matrinxã mortality have been associated with abrupt decrease in the water temperature. Thus, we subjected matrinxã to a sudden cold shock by transferring the fish directly to tanks in which the water temperature was 10 o C below the initial conditions (cold shock from 28°C to 18 o C). After 1h the fish were returned to the original tanks (28ºC). The handling associated with tank transfer was also imposed on control groups (not exposed to cold shock). while exposure to cold shock did not alter the measured physiological conditions within 1h, fish returned to the ambient condition (water at 28º C) significantly increased plasma cortisol and glucose levels. exposure to cold shock and return to the warm water did not affect HsP70 levels. The increased plasma cortisol and glucose levels after returning the fish to warm water suggest that matrinxã requires cortisol and glucose for adaptation to increased temperature.Key words: matrinxã, cold shock, stress, HsP70.Respostas fisiológicas ao estresse do peixe de águas tépidas matrinxã (Brycon amazonicus) submetido à queda brusca de temperatura resumo o presente trabalho avaliou as principais respostas fisiológicas e celulares [endócrino (cortisol), metabólico (glicose), hematológico (hematócrito and hemoglobina) e celular (HsP70)] ao estresse de um peixe de águas tépidas, o matrinxã (Brycon amazonicus), quando submetido a um choque térmico frio abrupto. essa espécie vem sendo amplamente cultivada na América do sul por apresentar excelentes índices zootécnicos de crescimento e conversão alimentar. entretanto, os produtores rurais encontram limitações no manejo do matrinxã, quando criado em regiões mais frias que sua região de origem, a Bacia Amazônica. Assim, o matrinxã foi submetido a um choque frio através da transferência direta dos peixes para tanques com água fria a 18°C. Após 1h, esses peixes retornaram a suas caixas de origem a 28°C. o manuseio de peixes necessário para conduzir o choque térmico experimental foi também imposto aos grupos controle, sendo, entretanto, evitada a água fria. o matrinxã demonstrou claros sinais de estresse fisiológico durante os procedimentos experimentais. Porém, essas respostas não foram associadas ao choque frio, mas sim ao choque quente por ocasião da volta dos peixes para as caixas de origem. As respostas primárias e secundárias de estresse foram evidentes através das análises plasmáticas de cortisol e glicose. Já o hematócrito, a hemoglobina e as expressões da proteína de estresse, HsP70, não foram afetadas. nossos resultados sustentam qu...

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Section: Discussionsupporting

confidence: 85%

Physiological stress responses in the warm-water fish matrinxã (Brycon amazonicus ) subjected to a sudden cold shock

et al. 2008

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“…; Ghanawi, Samer & Imad ) . However, some fish species required high concentration (140–150 mg L −1 ) of MS‐222 to achieve desirable induction (≤3 min) and recovery (≤5 min) compare with this species (Roubach, De Carvalho & Val ; Mercy, Malika & Sajan ; Mitjana, Bonastre, Insua, Falceto, Esteban, Josa & Espinosa ). Similarly, MS‐222 efficacy for induction (≤ 3 min) and recovery (≤ 5 min) was found in low concentration (100 mg L −1 ) in another fish species than Labeo rohita (Hseu, Yeh, Chu & Ting ; Bystriansky, LeBlanc & Ballantyne ; Ibarra‐Zatarain, Ibarra‐Castro, Alvarez‐Lajonchère, Aguilar & Sánchez‐Téllez ).…”

Section: Discussionmentioning

confidence: 99%

Anaesthetic efficacy of MS-222 and AQUI-S^®in advanced size fry of rohu,Labeo rohita,(Hamilton-Buchanan)

Husen

Sharma

2015

Aquac Res

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Anaesthetic agents are very useful for reducing the stress caused by handling, sorting, transportation, artificial reproduction, tagging, administration of vaccines and surgical procedures in fish. The efficacy of two anaesthetics: MS-222 and AQUI-S â were tested on rohu, Labeo rohita advanced size fry. The lowest effective doses that produced induction in 3 min or less and recovery times 5 min or less and meet the most criteria of good anaesthetic characteristics were 125 mg L À1 of MS-222, and 30 mg L À1 of AQUI-S â in rohu, Labeo rohita advanced size fry. Induction times were significantly decreased with increased in the concentrations of any of the two tested anaesthetic agents. The lowest doses suitable for transportation of rohu advanced size fry observed were: 10-15 mg L À1 of MS-222 and 2.5 mg L À1 AQUI-S â . Both anaesthetics showed promising to be used as anaesthetics for handling and transportation in rohu (Labeo rohita) advanced fry.

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“…Entretanto, a análise do cortisol plasmático em peixes nas condições brasileiras é muitas vezes economicamente pouco viável. Roubach et al (2001) detectaram aumento adicional de glicemia em matrinxã, quando submetido a banho anestésico com MS 222 em concentrações acima de 150 mg L -1 por 10 min. Em outro estudo, a benzocaína proporcionou redução da hiperglicemia plasmática em matrinxã durante banhos anestésicos em concentrações de até 60 mg L -1 por 10 min (Inoue et al 2004).…”

Section: Avaliação De Respostas Metabólicas Do Tambaqui Exposto Ao Euunclassified

Avaliação de respostas metabólicas do tambaqui exposto ao eugenol em banhos anestésicos

et al. 2011

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ResumoO tambaqui é a principal espécie de peixe cultivado na Amazônia Ocidental. Porém durante o processo produtivo, práticas de manejo são necessárias para o monitoramento do crescimento e estado geral da sanidade dos animais. Para isso os animais devem ser anestesiados para maior segurança no trabalho. O eugenol, componente majoritário do óleo de cravo, tem sido bastante utilizado como anestésico alternativo para peixes por ser um produto natural e de baixo custo. Entretanto, estudos tratando de respostas metabólicas em peixes tropicais expostos a diferentes anestésicos são ainda necessários. Dentro desse intuito, o presente trabalho avaliou respostas metabólicas, detectadas por meio de alterações de parâmetros sanguíneos e plasmáticos do tambaqui, exposto ao eugenol em banhos anestésicos simulados. Respostas típicas ao estresse foram detectadas devido ao manuseio imposto aos peixes durante a realização dos banhos anestésicos. O eugenol não reduziu totalmente essas reações ao estresse. Por outro lado, esse anestésico não provocou estresse adicional em exposições curtas de 15 min em concentrações próximas a 20 mg L -1 . PalavRas-Chave: Colossoma macropomum, respostas fisiológicas, hematologia. Evaluation of tambaqui metabolic responses to eugenol in anesthetics bathsabstRaCt Tambaqui is the main farmed fish in the Western Amazon. However, in handling this fish has to be anesthetized for safety purposes, usually when evaluating growth and health conditions. Eugenol, the main component of clove oil, has been reported as an alternative fish anesthetic, because it is an inexpensive natural product. However, continued studies are necessary about the metabolic responses of tropical fish to anesthetics. The present work evaluated metabolic responses of tambaqui to eugenol in simulated anesthetic baths, measuring blood and plasma parameters. Typical metabolic stress responses to handling were detected, but they were not totally reduced by eugenol. On the other hand, the anesthetic dissolved in water did not provoke any extra charge of stress during short-term exposures in concentrations of about 20mg L -1 for 15 min.

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Safest level of tricaine methanesulfonate (ms-222) to induce anesthesia in juveniles of Matrinxã, Brycon cephalus

Cited by 34 publications

References 9 publications

Physiological stress responses in the warm-water fish matrinxã (Brycon amazonicus ) subjected to a sudden cold shock

Physiological stress responses in the warm-water fish matrinxã (Brycon amazonicus ) subjected to a sudden cold shock

Anaesthetic efficacy of MS-222 and AQUI-S^®in advanced size fry of rohu,Labeo rohita,(Hamilton-Buchanan)

Avaliação de respostas metabólicas do tambaqui exposto ao eugenol em banhos anestésicos

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Safest level of tricaine methanesulfonate (ms-222) to induce anesthesia in juveniles of Matrinxã, Brycon cephalus

Cited by 34 publications

References 9 publications

Physiological stress responses in the warm-water fish matrinxã (Brycon amazonicus ) subjected to a sudden cold shock

Physiological stress responses in the warm-water fish matrinxã (Brycon amazonicus ) subjected to a sudden cold shock

Anaesthetic efficacy of MS-222 and AQUI-S®in advanced size fry of rohu,Labeo rohita,(Hamilton-Buchanan)

Avaliação de respostas metabólicas do tambaqui exposto ao eugenol em banhos anestésicos

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Anaesthetic efficacy of MS-222 and AQUI-S^®in advanced size fry of rohu,Labeo rohita,(Hamilton-Buchanan)