Safety and efficacy of an inactivated Carbopol-adjuvanted goose haemorrhagic polyomavirus vaccine for domestic geese

Abstract

Haemorrhagic nephritis enteritis of the goose (HNEG) is an epizootic viral disease in domestic geese. The causal agent is a polyomavirus, namely goose haemorrhagic polyomavirus. To help control the disease, an inactivated vaccine was developed, based on viral particles produced in goose kidney cells. Viral material was quantified using real-time quantitative polymerase chain reaction, inactivated with b-propiolactone and adjuvanted with Carbopol, an acrylic acid polymer. Carbopol proved to be more immunogenic than aluminium hydroxide and was totally safe when administered to young goslings and breeders alike. Carbopol-adjuvanted vaccine induced a high serological response. Moreover, goslings hatched from vaccinated breeders were protected against viral challenge, indicating that maternally-derived neutralizing antibodies (MDA) were efficiently transferred. MDA were still detectable 15 days post-hatch. Clinical trials will be necessary to accurately evaluate a vaccine-based HNEG control strategy under field conditions.

Propagation of viruses infecting waterfowl on continuous cell lines of Muscovy duck (Cairina moschata) origin

“…GHPV also produced relatively low virus titres with variable copy numbers (10 4 to 10 7 copies/ml) ( Table 4). This production lags behind primary goose kidney cells of 1-day-old goslings, which were reported to produce 10 11 GHPV genome/ml under special circumstances (Gelfi et al, 2010).…”

Visual detection of goose haemorrhagic polyomavirus in geese and ducks by loop-mediated isothermal amplification

“…Oedema of subcutaneous tissues and gelatinous ascites are also observed, which are related to the selective tropism of GHPV to the endothelial cells (Schettler, 1980, Lacroux et al, 2004. Currently, there is no commercially accessible vaccine against HNEG but previous studies conducted by Mato et al (2004) as well as Gelfi et al (2010) suggested that double vaccination of geese with subunit vaccine elicited 100% protection (Mato et al, 2009). Taking into account the lack of HNEG prophylaxis early GHPV detection seems to be important for biosecurity of waterfowl.…”

Pathological and epidemiological significance of Goose haemorrhagic polyomavirus infection in ducks

“…Transmission of maternal GHPV antibodies was previously assessed in domestic geese (Gelfi et al, 2010). Thus, it was suspected that GHPV may circulate among breeding duck farms.…”

“…A fourth inoculum was purified from a cell culture as previously described (Guerin et al, 2000). GHPV titres were determined by real-time quantitative PCR analysis as previously described (Gelfi et al, 2010) and expressed as genomeequivalent viruses (gev).…”

“…Quantitative ELISA titres were expressed as the inverse of the dilution giving an OD value three times as great as the OD value of the standard negative control, according to standard procedures (Gelfi et al, 2010). A titre of less than 20 was considered negative.…”