Safety and efficacy of G2-S16 dendrimer as microbicide in healthy human vaginal tissue explants

Rodriguez-Izquierdo, Ignacio; Serramía, Ma Jesús; Gómez, Rafael; Espinosa, G.; Genebat, M.; Leal, Manuel; Muñoz‐Fernández, María Ángeles

doi:10.1186/s12951-022-01350-8

Cited by 5 publications

(3 citation statements)

References 34 publications

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“…The limitations of traditional antiviral therapies against HCMV infection have led to the development of novel treatments, including genetic, cellular and immune therapies [20]. Among these alternatives, dendrimers have demonstrated efficacy against different pathogens, including HCMV [21][22][23]. Our study highlights the combined immune effect of PCDs G2-S16 and G2-S24P with the immunomodulatory capacity of Tα1, showing their ability to enhance the functionality of DCs as antigen-presenting cells (APCs) and subsequently boost the activation of T cells, including Treg, promoting an adaptive response against HCMV infection.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Immunomodulatory Effects of Thymosin-Alpha-1 in Combination with Polyanionic Carbosilane Dendrimers against HCMV Infection

Espinar-Buitrago,

Magro-López,

Vázquez-Alejo

et al. 2024

IJMS

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Resistance and toxicity associated with current treatments for human cytomegalovirus (HCMV) infection highlight the need for alternatives and immunotherapy has emerged as a promising strategy. This study examined the in vitro immunological effects of co-administration of Thymosin-alpha-1 (Tα1) and polyanionic carbosilane dendrimers (PCDs) on peripheral blood mononuclear cells (PBMCs) during HCMV infection. The biocompatibility of PCDs was assessed via MTT and LDH assays. PBMCs were pre-treated with the co-administered compounds and then exposed to HCMV for 48 h. Morphological alterations in PBMCs were observed using optical microscopy and total dendritic cells (tDCs), myeloid dendritic cells (mDCs), and plasmacytoid dendritic cells (pDCs), along with CD4+/CD8+ T cells and regulatory T cells (Treg), and were characterized using multiparametric flow cytometry. The findings revealed that Tα1 + PCDs treatments increased DC activation and maturation. Furthermore, increased co-receptor expression, intracellular IFNγ production in T cells and elevated Treg functionality and reduced senescence were evident with Tα1 + G2-S24P treatment. Conversely, reduced co-receptor expression, intracellular cytokine production in T cells, lower functionality and higher senescence in Treg were observed with Tα1 + G2S16 treatment. In summary, Tα1 + PCDs treatments demonstrate synergistic effects during early HCMV infection, suggesting their use as an alternative therapeutic for preventing virus infection.

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Section: Discussionmentioning

confidence: 99%

Enhanced Immunomodulatory Effects of Thymosin-Alpha-1 in Combination with Polyanionic Carbosilane Dendrimers against HCMV Infection

Espinar-Buitrago,

Magro-López,

Vázquez-Alejo

et al. 2024

IJMS

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“…Summary of main in vivo assays performed with polyanionic carbosilane dendrimer G2‐S16 as vaginal microbicides against HSV. Reprinted with permission from Rodríguez‐Izquierdo, Serramía, et al (2022). Copyright© 2022, Wiley…”

Section: Pharmaceutical Applications Of Carbosilane Dendritic Moleculesmentioning

confidence: 99%

Carbosilane dendritic nanostructures, highly versatile platforms for pharmaceutical applications

Mata

Gomez-Ramirez

Cano

et al. 2022

WIREs Nanomed Nanobiotechnol

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Dendrimers are multifunctional molecules with well‐defined size and structure due to the step‐by‐step synthetic procedures required in their preparation. Dendritic constructs based on carbosilane scaffolds present carbon–carbon and carbon–silicon bonds, which results in stable, lipophilic, inert, and flexible structures. These properties are highly appreciated in different areas, including the pharmaceutical field, as they can increase the interaction with cell membranes and improve the therapeutic action. This article summarizes the most recent advances in the pharmaceutical applications of carbosilane dendritic molecules, from therapeutics to diagnostics and prevention tools. Dendrimers decorated with cationic, anionic, or other moieties, including metallodendrimers; supramolecular assemblies; dendronized nanoparticles and surfaces; as well as dendritic networks like hydrogels are described. The collected examples confirm the potential of carbosilane dendrimers and dendritic materials as antiviral or antibacterial agents; in therapy against cancer, neurodegenerative disease, or oxidative stress; or many other biomedical applications. This article is categorized under: Nanotechnology Approaches to Biology > Nanoscale Systems in Biology Therapeutic Approaches and Drug Discovery > Nanomedicine for Infectious Disease Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease

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“…[ 82 ] AuNPs have the capacity to cross de blood–brain barrier in mice model, without cerebral damage, and inhibiting HSV‐1 infection. [ 83 ] Interestingly, the authors reported that AuNPs also reduced HSV‐1 induced β‐secretase activity, which is related to Alzheimer's disease. [ 84 ] Copper nanoparticles (CuNPs) at 100 µg mL −1 significantly decreased virus titers (HSV‐1) with an inhibition rate of 83.3%.…”

Section: Human Viral Diseases and Nanoparticlesmentioning

confidence: 99%

Advanced Material Against Human (Including Covid‐19) and Plant Viruses: Nanoparticles As a Feasible Strategy

et al. 2020

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The SARS‐CoV‐2 virus outbreak revealed that these nano‐pathogens have the ability to rapidly change lives. Undoubtedly, SARS‐CoV‐2 as well as other viruses can cause important global impacts, affecting public health, as well as, socioeconomic development. But viruses are not only a public health concern, they are also a problem in agriculture. The current treatments are often ineffective, are prone to develop resistance, or cause considerable adverse side effects. The use of nanotechnology has played an important role to combat viral diseases. In this review three main aspects are in focus: first, the potential use of nanoparticles as carriers for drug delivery. Second, its use for treatments of some human viral diseases, and third, its application as antivirals in plants. With these three themes, the aim is to give to readers an overview of the progress in this promising area of biotechnology during the 2017–2020 period, and to provide a glance at how tangible is the effectiveness of nanotechnology against viruses. Future prospects are also discussed. It is hoped that this review can be a contribution to general knowledge for both specialized and non‐specialized readers, allowing a better knowledge of this interesting topic.

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Safety and efficacy of G2-S16 dendrimer as microbicide in healthy human vaginal tissue explants

Cited by 5 publications

References 34 publications

Enhanced Immunomodulatory Effects of Thymosin-Alpha-1 in Combination with Polyanionic Carbosilane Dendrimers against HCMV Infection

Enhanced Immunomodulatory Effects of Thymosin-Alpha-1 in Combination with Polyanionic Carbosilane Dendrimers against HCMV Infection

Carbosilane dendritic nanostructures, highly versatile platforms for pharmaceutical applications

Advanced Material Against Human (Including Covid‐19) and Plant Viruses: Nanoparticles As a Feasible Strategy

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