Safety assessment of different unloaded polymeric nanocapsules in Caenorhabditis elegans

Agarrayua, Danielle Araújo; Funguetto-Ribeiro, Ana Cláudia; Trevisan, Paula; Haas, Sandra Elisa; Ávila, Daiana Silva

doi:10.1016/j.cbpc.2022.109477

Cited by 5 publications

(2 citation statements)

References 40 publications

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“…Furthermore, SEM analysis revealed uniformly dispersed spheroidal structures by the hydrogel. Similarly, Agarrayua et al 37 developed nanocapsules with a similar structure, obtaining values of satisfactory size (185 nm) and PDI (0.11). In another study, laser diffraction analysis showed lipid‐core nanocapsules with unimodal distribution (span below 2) and particles in the nanometer range (d4.3 = 184 nm), results similar to our findings 38 …”

Section: Resultsmentioning

confidence: 95%

Successful preparation of a biopolymeric nanocarrier enables controlled release of antinociceptive monoterpene for local pain management after transdermal barrier permeation

de Araujo Andrade,

de Jesus,

de Sousa Pereira

et al. 2024

J of Applied Polymer Sci

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Developing effective and targeted drug delivery systems is crucial in searching for improved pain management strategies. Here, it is shown the preparation of a nanocarrier using chitosan hydrogel, and polymeric nanocapsules loaded with linalool for enhancing transdermal permeation and targeted delivery of this antinociceptive monoterpene for the pain treatment. Extensive characterization was conducted to evaluate the properties of the nanocarrier, and several in vitro studies were performed to investigate the release kinetics of linalool from the nanocarrier and its permeation through the layers of the skin. As results, the prepared polymeric nanocarrier exhibited an average size of 160 ± 9 nm, with a polydispersion index of 0.12 ± 0.01. The kinetic study revealed that the nanocarrier effectively controlled and prolonged the release of linalool, following a pseudo‐second order model (R2 = 0.98). To evaluate the permeation of the nanocarrier through the transdermal barrier, swine ear skin was employed. The nanocarrier efficiently penetrated the transdermal barrier and successfully delivered linalool to the skin layers. Additionally, an in vivo toxicity study indicated no toxicity for the nanocarrier at the tested concentrations (<0.950 μg mL−1). The release kinetics showed a controlled and sustained release of the linalool, suggesting its potential for prolonged therapeutic effects.

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Section: Resultsmentioning

confidence: 95%

Successful preparation of a biopolymeric nanocarrier enables controlled release of antinociceptive monoterpene for local pain management after transdermal barrier permeation

de Araujo Andrade,

de Jesus,

de Sousa Pereira

et al. 2024

J of Applied Polymer Sci

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“…Particularly in nanotoxicology, some features are important for its successful application in this field [ 41 ]. It is a small and non-parasitic worm, therefore it has a low cost maintenance, making possible to grow billions of worms in a small space and the amount of nanoparticles needed to be tested are much lower in comparison to other in vivo models; the life and reproductive cycles are short, which allows for the observation of lifespan alterations, reproductive damage and transgenerational effects in less than one month [ 41 , 42 , 43 ]; their genome is fully sequenced and it is known that C. elegans conserves a genetic identity with human genes of 41.7% (20–71% in a range) and several cellular processes are highly similar between the species, therefore making possible to extrapolate the findings from an invertebrate to mammals [ 44 ]; the easy genetic manipulation that allows the construction of deletion mutants or fluorescent tagged proteins to investigate the toxicity mechanism of the nanoparticles [ 45 ]; they are transparent, which facilitates the visualization of the organs, nanoparticles internalization and cellular markers [ 46 ]. Notably, this nematode is extremely sensitive to the adverse effects of the environment where it is found, changing its morphology, behavior, and cellular redox state [ 47 ].…”

Section: Caenorhabditis Elegansmentioning

confidence: 99%

Caenorhabditis elegans as a Prediction Platform for Nanotechnology-Based Strategies: Insights on Analytical Challenges

Gubert

Oliveira

et al. 2023

Toxics

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Nanotechnology-based strategies have played a pivotal role in innovative products in different technological fields, including medicine, agriculture, and engineering. The redesign of the nanometric scale has improved drug targeting and delivery, diagnosis, water treatment, and analytical methods. Although efficiency brings benefits, toxicity in organisms and the environment is a concern, particularly in light of global climate change and plastic disposal in the environment. Therefore, to measure such effects, alternative models enable the assessment of impacts on both functional properties and toxicity. Caenorhabditis elegans is a nematode model that poses valuable advantages such as transparency, sensibility in responding to exogenous compounds, fast response to perturbations besides the possibility to replicate human disease through transgenics. Herein, we discuss the applications of C. elegans to nanomaterial safety and efficacy evaluations from one health perspective. We also highlight the directions for developing appropriate techniques to safely adopt magnetic and organic nanoparticles, and carbon nanosystems. A description was given of the specifics of targeting and treatment, especially for health purposes. Finally, we discuss C. elegans potential for studying the impacts caused by nanopesticides and nanoplastics as emerging contaminants, pointing out gaps in environmental studies related to toxicity, analytical methods, and future directions.

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Alternatives of Animal Models for Biomedical Research: a Comprehensive Review of Modern Approaches

Vashishat,

Patel,

Das Gupta

et al. 2024

Stem Cell Rev and Rep

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Safety assessment of different unloaded polymeric nanocapsules in Caenorhabditis elegans

Cited by 5 publications

References 40 publications

Successful preparation of a biopolymeric nanocarrier enables controlled release of antinociceptive monoterpene for local pain management after transdermal barrier permeation

Successful preparation of a biopolymeric nanocarrier enables controlled release of antinociceptive monoterpene for local pain management after transdermal barrier permeation

Caenorhabditis elegans as a Prediction Platform for Nanotechnology-Based Strategies: Insights on Analytical Challenges

Alternatives of Animal Models for Biomedical Research: a Comprehensive Review of Modern Approaches

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