Safety characterization of Yersinia enterocolitica strains isolated from raw milk in Western Algeria

Hadef, Kh. Z.; Medjahdi, Kh.; Beldjilali, H.; Benamar, Ibrahim

doi:10.5897/ajmr2015.7839

Cited by 3 publications

(1 citation statement)

References 32 publications

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“…According to our findings, every isolated strain was resistant to AMC, then cefazolin (92.3%) and ampicillin (65.4%), that result agrees with many authors [41,42]. One the other side, the isolated strains were sensitive to gentamicin and ciprofloxacin (96.2%) then, trimethoprim/ sulfamethoxazole and chloramphenicol (92.3%) finally, kanamycin and cephalothin (84.6%), that result agrees with many researchers [42,43]. There must be a change in how antibiotics are used globally.…”

Section: Discussionsupporting

confidence: 90%

Virulence Genotyping Profiles of Cefazolin Resistance Yersinia enterocolitica Isolated From Milk and Milk Products in Egypt

Dowidar

Khalifa

2023

Egyptian Journal of Veterinary Sciences

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T HE aim of this study was to investigate the incidence rate, characteristics, genes related with virulence, and determination of antibiotic resistance of pathogenic Yersinia (Y.) enterocolitica isolated from raw milk, Karish cheese, and ice cream. From July 2021 to May 2022, samples were obtained from popular markets, and street vendors in various places in Mansoura city, Dakahlia, Egypt. Milk, Karish cheese, and ice cream (50 samples each) were positive in 26 of 150 samples (17.3%) for Yersinia enterocolitica, with a higher prevalence in ice cream (26%). Among the 26 Y. enterocolitica isolates, three virulence genes (ail, inv, and yadA) had different frequency profiles. The inv gene was found in a significant majority of the strains tested (73.1%), followed by the ail gene (50%) and the yadA gene (26.9%). Gentamicin and ciprofloxacin were estimated to have a high sensitivity rate ((96.2% each), followed by sulphamethoxazol-trimethoprim and chloramphenicol (92.3%), then cephalotin and kanamycin (84.6%), finally fosfomycin (73.1%). Interestingly, 53.8% of strains were identified as the multidrug resistance, with 11 different resistance patterns. Nineteen strains of Y. enterocolitica showed biofilm-forming ability, of which seven strains had the high biofilm-forming capacity and eight other strains of Y. enterocolitica had moderate biofilmforming capacity. The discovery of presumably pathogenic Y. enterocolitica isolates from milk and milk products implies a hazard to consumer safety especially due to the occurrence of pathogenicity potential and antibiotic resistance which necessity the implementation of control measures.

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Section: Discussionsupporting

confidence: 90%

Virulence Genotyping Profiles of Cefazolin Resistance Yersinia enterocolitica Isolated From Milk and Milk Products in Egypt

Dowidar

Khalifa

2023

Egyptian Journal of Veterinary Sciences

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Microbial quality and safety of milk and milk products in the 21st century

Fusco

Chieffi

Fanelli

et al. 2020

Comp Rev Food Sci Food Safe

152

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Milk and milk products have been utilized by humans for many thousands of years. With the advent of metagenomic studies, our knowledge on the microbiota of milk and milk products, especially as affected by the environment, production, and storage parameters, has increased. Milk quality depends on chemical parameters (fat and protein content and absence of inhibitory substances), as well as microbial and somatic cells counts, and affects the price of milk. The effects of hygiene and effective cooling on the spoilage microbiota have shown that proteolytic and lipolytic bacteria such as Pseudomonas or Acinetobacter spp. predominate the spoilage bacterial populations. These bacteria can produce heat‐stable proteases and lipases, which remain active after pasteurization and thus can spoil the milk during prolonged storage. Additionally, milk can become contaminated after pasteurization and therefore there is still a high demand on developing better cleaning and sanitation regimes and equipment, as well as test systems to (quantitatively) detect relevant pathogenic or spoilage microorganisms. Raw milk and raw milk cheese consumption is also increasing worldwide with the growing demand of minimally processed, sustainable, healthy, and local foods. In this context, emerging and re‐emerging pathogens once again represent a major food safety challenge. As a result of global warming, it is conceivable that not only microbiological risks but also chemical risks relating to presence of mycotoxins or plant toxins in milk will increase. Herein, we provide an overview of the major microbial hazards occurring in the 21st century.

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Isolation and characterization of Yersinia enterocolitica from foods in Apulia and Basilicata regions (Italy) by conventional and modern methods

et al. 2022

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Yersiniosis is the third most reported food-borne zoonosis in Europe. The aim of the present study was to perform the search for Yersinia enterocolitica in food samples collected from Apulia and Basilicata regions (Southern Italy) and to characterize any isolates by classical and modern analytical methods. A total of 130 samples were analyzed between July 2018 and July 2019: most of them were raw milk and dairy products made from it. Furthermore, 8 out of 130 samples were individual milk samples collected from bovines reared in a Brucella-free farm which showed false positive serological reaction for brucellosis due to the presence of pathogenic Y. enterocolitica O:9 biotype 2 in faeces. The Real Time PCR targeting the ail gene and the culture method were performed to detect pathogenic Y. enterocolitica. Isolates were subjected to API 20E (Biomerieux) and MALDI-TOF MS (Matrix Assisted Laser Desorption Ionization Time-of-Flight) for species identification. All samples were negative for the ail gene. The culture method allowed to isolate suspicious colonies from 28 samples. The API 20E system and the MALDI-TOF MS technique identified 20 Y. enterocolitica and 1 Y. intermedia in a concordant way. The remaining 7 strains were all identified as Y. enterocolitica by the API 20E system, while the MALDI-TOF MS recognized 4 Y. intermedia, 1 Y. bercovieri and 2 Y. massiliensis. Genotypic characterization of the discordant strains was performed by rMLST and it confirmed the MALDI-TOF MS’ results. Only non-pathogenic Y. enterocolitica biotype 1A strains were found, although with a non-negligible prevalence (P = 0.15 with CI 95% = ± 0.06). This study indicates a poor circulation of pathogenic Y. enterocolitica in food products made and marketed in the investigated areas. However, the small number of samples, insufficient for some food categories such as meat and vegetable, does not allow to exclude the presence of pathogenic strains at all.

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Safety characterization of Yersinia enterocolitica strains isolated from raw milk in Western Algeria

Cited by 3 publications

References 32 publications

Virulence Genotyping Profiles of Cefazolin Resistance Yersinia enterocolitica Isolated From Milk and Milk Products in Egypt

Virulence Genotyping Profiles of Cefazolin Resistance Yersinia enterocolitica Isolated From Milk and Milk Products in Egypt

Microbial quality and safety of milk and milk products in the 21st century

Isolation and characterization of Yersinia enterocolitica from foods in Apulia and Basilicata regions (Italy) by conventional and modern methods

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