Safety Evaluation of a Novel Potentially Probiotic Limosilactobacillus fermentum in Rats

Neto, José Patrocínio Ribeiro Cruz; Oliveira, Alison Macário de; Oliveira, Kataryne Árabe Rimá de; Sampaio, Karoliny Brito; Dutra, Maria Letícia da Veiga; Freire, Micaelle Oliveira de Luna; Souza, Evandro Leite de; Alves, José Luiz

doi:10.1007/s12602-023-10077-3

Cited by 5 publications

(1 citation statement)

References 48 publications

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“…A mixed-fruit-derived Limosilactobacillus fermentum developed by our research group has been reported as safe in a series of in vitro and in vivo experiments [20] and as having broad probiotic properties, such as the normalization of disturbed gut microbiota and antioxidant and anti-inflammatory properties when administered at 1 × 10 9 CFU/day [21][22][23][24]. However, it is unclear whether mixed L. fermentum can modulate the gut microbiota in a dose-and sexspecific manner.…”

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confidence: 99%

A Mix of Potentially Probiotic Limosilactobacillus fermentum Strains Alters the Gut Microbiota in a Dose- and Sex-Dependent Manner in Wistar Rats

Carneiro dos Santos,

Carvalho,

Cruz Neto

et al. 2024

Microorganisms

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Multi-strain Limosilactobacillus (L.) fermentum is a potential probiotic with reported immunomodulatory properties. This study aimed to evaluate the composition, richness, and diversity of the gut microbiota in male and female rats after treatment with a multi-strain of L. fermentum at different doses. Thirty rats (fifteen male and fifteen female) were allocated into a control group (CTL), a group receiving L. fermentum at a dose of 108 CFU (Lf-108), and a group receiving L. fermentum at a dose of 1010 CFU (Lf-1010) for 13 weeks. Gut microbiota and serum cytokine levels were evaluated after L. fermentum treatment. Male CTL rats had a lower relative abundance of Bifidobacteriaceae and Prevotella and a lower alpha diversity than their female CTL counterparts (p < 0.05). In addition, male CTL rats had a higher Firmicutes/Bacteroidetes (F/B) ratio than female CTL rats (p < 0.05). In female rats, the administration of L. fermentum at 108 CFU decreased the relative abundance of Bifidobacteriaceae and Anaerobiospirillum and increased Lactobacillus (p < 0.05). In male rats, the administration of L. fermentum at 1010 CFU decreased the F/B ratio and increased Lachnospiraceae and the diversity of the gut microbiota (p < 0.05). The relative abundance of Lachnospiraceae and the alpha-diversity of gut microbiota were negatively correlated with serum levels of IL1β (r = −0.44) and TNFα (r = −0.39), respectively. This study identified important changes in gut microbiota between male and female rats and showed that a lower dose of L. fermentum may have more beneficial effects on gut microbiota in females, while a higher dose may result in more beneficial effects on gut microbiota in male rats.

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