Safety of a live attenuated Erysipelothrix rhusiopathiae vaccine for swine

Neumann, E.; Grinberg, Alex; Bonistalli, Kathryn N.; Mack, Hamish J; Lehrbach, P. R.; Gibson, Nicole

doi:10.1016/j.vetmic.2008.09.059

Cited by 9 publications

(3 citation statements)

References 10 publications

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“…Attenuated E. rhusiopathiae vaccines have long been used for oral or nasal immunization of turkeys (20) and pigs (21,22) and for subcutaneous immunization of pigs in a Pigs were inoculated orally with 10 10 CFU of the strains in milk replacer for 3 consecutive days and challenged with 10 8 CFU of the Fujisawa strain on day 16 after the final vaccination. b The agglutination titer was determined as described in the footnote of Table 2.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of Virulence Genes in Erysipelothrix rhusiopathiae : Use of a Mutant Deficient in a tagF Homolog as a Safe Oral Vaccine against Swine Erysipelas

et al. 2019

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Swine erysipelas is caused by the Gram-positive pathogen Erysipelothrix rhusiopathiae. The swine erysipelas live vaccine in Japan, the E. rhusiopathiae Koganei 65-0.15 strain (Koganei), has been reported to cause arthritis and endocarditis. To develop a vaccine with increased safety, we used a virulent Fujisawa strain to construct transposon mutants for a total of 651 genes, which covered 38% of the coding sequence of the genome. We screened the mutants for attenuation by inoculating mice with 108 CFU of each mutant and subsequently assessed protective capability by challenging the surviving mice with 103 CFU (102 times the 50% lethal dose) of the Fujisawa strain. Of the 23 attenuated mutants obtained, 6 mutants were selected and evaluated for protective capability in pigs by comparison to that of the Koganei strain. A mutant in the ERH_0432 (tagF) gene encoding a putative CDP-glycerol glycerophosphotransferase was found to be highly attenuated and to induce humoral and cell-mediated immune responses in conventional pigs. An in-frame deletion mutant of the gene, the Δ432 mutant, was constructed, and attenuation was further confirmed in germfree piglets; three of four piglets subcutaneously inoculated with 109 CFU of the Δ432 mutant showed no apparent clinical symptoms, whereas all four of the Koganei-inoculated piglets died 3 days after inoculation. It was confirmed that conventional pigs inoculated orally or subcutaneously with the Δ432 strain were almost completely protected against lethal challenge infection. Thus, the tagF homolog mutant of E. rhusiopathiae represents a safe vaccine candidate that can be administered via the oral and subcutaneous routes.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of Virulence Genes in Erysipelothrix rhusiopathiae : Use of a Mutant Deficient in a tagF Homolog as a Safe Oral Vaccine against Swine Erysipelas

et al. 2019

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“…Specifically, oral vaccines were found to be safe in pigs without generating carriers or shedders (76) and vaccines were found to be stable both in the dried and liquid stage (72). Subsequently, swine oral vaccines were also examined for use in turkeys and were considered potentially useful (77).…”

Section: Attenuated Vaccinesmentioning

confidence: 99%

Erysipelothrix Spp.: Past, Present, and Future Directions in Vaccine Research

2020

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“…Due to the high frequency of occurrence of these outbreaks, there is a suspicion that the vaccine composed of the attenuated bacterium may have its virulence reversed. However, it is difficult to prove that the strain present in the vaccine was the same responsible for the outbreak of disease, therefore vaccines composed by antigenic parts of the pathogenic agent could be a promising alternative to provide higher protection and safety [11,19,20].…”

Section: Introductionmentioning

confidence: 99%

Immunogenicity and Protection by DnaK and SpaA Recombinant Proteins Against Erysipelothrix Rhusiopathiae in a Murine Model

2023

Cell Physiol Biochem

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Background/Aims: Swine erysipelas is a disease caused by Erysipelothrix rhusiopathiae , a Gram-positive bacillus, which has great economic importance because it leads to the loss of the swine herd. To control this disease, animals are immunized with a cellular vaccine of killed or attenuated E. rhusiopathiae , but even with herd vaccination, cases of swine erysipelas outbreaks have been reported in the United States, China and Japan, leading to the search for other antigenic components of the bacteria that may promote greater protection against E. rhusiopathiae . The surface protein SpaA from E. rhusiopathiae has been shown to be a candidate to constitute a subunit vaccine, since it has already been reported to induce a host immune response against the bacterium. DnaK, a hsp70 molecular chaperone, also seems to be a good candidate in the composition of a vaccine, as it has been demonstrated to be an antigenic protein of the bacteria. Methods: This work evaluated the immunogenicity and protection induced by the E. rhusiopathiae SpaA and DnaK recombinant proteins in a murine model, by intramuscular administration to mice with two doses of 100 µg at 21-day interval between them. The candidate proteins were tested either separately and together, compared with the commercial vaccine and the non-vaccination condition, and mice were challenged with a virulent strain of E. rhusiopathiae. Serum was collected to assess the produced antibodies and peripheral blood cells, whereas spleen and kidney tissues were assayed for E. rhusiopathiae presence by colony counting. Results: A survival curve of the animals was performed, which confirmed the protection induced by the proteins. IgG antibodies increased in the animal serum inoculated with the proteins when compared to the control, and a significant delay in disease symptoms was observed. Conclusion: These results suggest that E. rhusiopathiae DnaK and SpaA are immunogenic in mice and interfere with the disease development.

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Safety of a live attenuated Erysipelothrix rhusiopathiae vaccine for swine

Cited by 9 publications

References 10 publications

Genome-Wide Identification of Virulence Genes in Erysipelothrix rhusiopathiae : Use of a Mutant Deficient in a tagF Homolog as a Safe Oral Vaccine against Swine Erysipelas

Genome-Wide Identification of Virulence Genes in Erysipelothrix rhusiopathiae : Use of a Mutant Deficient in a tagF Homolog as a Safe Oral Vaccine against Swine Erysipelas

Erysipelothrix Spp.: Past, Present, and Future Directions in Vaccine Research

Immunogenicity and Protection by DnaK and SpaA Recombinant Proteins Against Erysipelothrix Rhusiopathiae in a Murine Model

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