Safety of bioabsorbable implants in vitro

Abstract

BackgroundThe aim of the present study was to investigate the safety of bioabsorbable plates and screws in humans.MethodsFor this purpose, an implant system based on [poly(lactic-co-glycolic acids)(85:15)] was designed. The system was tested for pH, temperature, and swelling and then its surface morphology was analyzed for surface porosity using environmental electron microscopy. Then, the effects of this bioabsorbable system on the viability and profileration of osteocytes were examined on a molecular level via in vitro experiments. A [poly(lactic-co-glycolic acids)(90:10)] bioabsorbable implant, which is commercially available and used in orthopedic surgery, was used as control group. For the statistical evaluation of the data obtained in the present study, the groups were compared by Tukey HSD test following ANOVA. The significance level was set as p < 0.05.ResultsIt was observed that the osteocytes cultivated on the PLGA system designed in the present study included more live cells and allowed more proliferation compared to the control.ConclusionOne of the criteria in the selection of implants for orthopedic surgery is that a good implant should not need removal and thus a second surgery. In the present study, a bioabsorbable implant was designed considering this criterion. The present study is the first step to prove the safety of this new design by in vitro toxicity and viability experiments.

Is implant washing and wound irrigation with rifampicin effective for preventing surgical site infections in lumbar instrumentation?

“…However, as is known, the sensitivity of human and animal tissues differs greatly, and may therefore generate misleading results. Additionally, as cell lines use single-type cells and do not have complex coordination mechanisms regarding the micro environments of cells and inhibit cell interactions, such as in the extracellular matrix, in vitro test results, which are already difficult to compare with in vivo conditions, become more controversial (7,8,15).…”

Can a Biodegradable Implanted Bilayered Drug Delivery System Loaded with BMP-2/BMP-12 Take an Effective Role in the Biological Repair Process of Bone–Tendon Injuries? A Preliminary Report

“…MTT analyses were evaluated in accordance with commercial kit bulletins on the 1st, 7th, 14th, and 21st days [15–20, 24, 26]. …”

Are We Economically Efficient Enough to Increase the Potential of in Vitro Proliferation of Osteoblasts by Means of Pharmacochemical Agents?

“…The supernatant was removed, and the cells were resuspended in an assay buffer and analyzed with a flow cytometer. Results were evaluated using the BD FACSCalibur Cell Quest software (Becton Dickinson, Palo Alto, CA, USA) [ 11 , 14 , 15 ].…”

“…The glutaraldehyde solution was then removed, and cells were maintained at room temperature for 2 h, prior to three washes using cacodylate buffer. After the last wash, the cells were covered with cacodylate buffer and stored at 4°C prior to analysis [ 14 , 15 , 17 , 18 ]. Images of the extracellular matrix and characteristic cellular structures were obtained.…”