Safflor yellow B suppresses pheochromocytoma cell (PC12) injury induced by oxidative stress via antioxidant systemand Bcl-2 /Bax pathway

Abstract

Our previous study demonstrates that SYB produces a neuroprotective effect in vivo. In the present study, we investigated the protective effect of safflor yellow B (SYB) on the acute oxidative injury induced by H(2)O(2) and mechanisms in PC12 cells. H(2)O(2) was used to mimic in vitro model of the oxidative injury and to induce apoptosis in PC12 cells. The cells were pretreated with the different concentrations of SYB. The cell viability, lactate dehydrogenase (LDH) release, malondialdehyde (MDA), and superoxide anion levels, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were measured. Caspase 3 activity, Bcl-2 and Bax expressions were also observed. The results showed that exposure of the cells to H(2)O(2) significantly decreased the cell viability, SOD and GSH-Px activities and Bcl-2 expression, and increased LDH release, superoxide anion and MDA generations, caspase 3 activity and Bax expressions. Pretreatment of the cells with SYB was able to remarkably antagonize the H(2)O(2)-induced changes in dose-dependent way. These suggest that SYB is able to protect PC12 cells from H(2)O(2)-induced injury and apoptosis via antioxidant and anti-apoptotic mechanisms.

l-3-n-butylphthalide alleviates hydrogen peroxide-induced apoptosis by PKC pathway in human neuroblastoma SK-N-SH cells

“…Our current study demonstrated that H 2 O 2 , as a prominent cause of apoptosis (Bissonnette et al 2004;Wang et al 2009), induced the typical apoptotic-like morphological changes in the human neuroblastoma SK-N-SH cells, such as the condensation of nuclear chromatin. When cells were pretreated with L-NBP, H 2 O 2 -induced apoptosis was significantly attenuated.…”

Safflower Yellow regulates microglial polarization and inhibits inflammatory response in LPS-stimulated Bv2 cells

“…29 In a series of experimental studies, SY enhanced Bcl-2 expression and inhibited Bax and caspase-3 activation, thus attenuating neuronal apoptosis and spinal cord ischemia reperfusion injury 16 and protected PC12 cells from H 2 O 2 -induced injury and apoptosis. 30 SY also inhibited neuroinflammation which was mediated by Aβ 14 and oxygen-glucose deprivation in BV-2 cells. 18 The mechanisms of SY action include the phosphorylation of JAK2/STAT3 pathway, 14 the upregulation of Bcl-2/p22 (phox) expression 15 as well as the inhibition of oxidative stress/apoptosis and NF-κB signaling pathway.…”

Effects of extracts and isolated compounds from safflower on some index of promoting blood circulation and regulating menstruation

“…However, the present study was focused on investigating the pharmacological actions of safflower extraction and yellow pigment of safflower, monomer as hydroxysafflor yellow A and anhydrosafflor yellow B (Chen et al, 2000;Zang et al, 2002;Zhao et al, 2009b;Wang et al, 2009;Lin et al, 2011;Pan et al, 2012;Nie et al, 2012;. More studies ought to be explored on the pharmacological effects and possible mechanisms of other constituents isolated from safflower.…”