Saikosaponin D inhibits proliferation of human osteosarcoma cells via the p53 signaling pathway

Abstract

Saikosaponin D (SSd), the major monomeric terpenoid extracted from Radix bupleuri, a traditional Chinese medicinal herb, exerts various pharmacological properties, including antitumor, anti-inflammatory and antiviral. The present study aimed to investigate the role of SSd in human osteosarcoma (OS) cell growth. In the investigation MTS and EdU assays were applied and flow cytometric analyses of cell cycle and apoptosis were performed. Western blotting and reverse transcription-quantitative polymerase chain reaction analyses were used to explore the underlying mechanisms of SSd on cell cycle transition and p53 signaling. Here, it was demonstrated that SSd administration at 80 µmol/l significantly inhibited 143B and MG-63 proliferation. Furthermore, SSd significantly increased the percentage of 143B and MG-63 cells in G 0-G 1 phase and the number of apoptosis cells compared with the control group. Data further demonstrated that SSd treatment upregulated mRNA and protein levels of tumor protein 53 (p53) and its downstream targets, including p21, p27, B-cell lymphoma-2-like protein 4 and cleaved caspase-3, and downregulated mRNA and protein levels of cyclinD1. The results suggested that SSd was a functional tumor suppressor and inhibited OS proliferation via activation of the p53 signaling pathway and may be used in the treatment of osteosarcoma in future.

Saikosaponin D improves chemosensitivity of glioblastoma by reducing the its stemness maintenance

“…Anti-tumor effect is the most important pharmacological activity of SSD [ 34 ]. Up to now, a large number of literatures have reported the inhibitory effects of SSD on a variety of tumors, including pancreatic cancer cells [ 10 ], hepatocellular carcinoma [ 35 ], renal cell carcinoma [ 36 ], human osteosarcoma [ 37 ], undifferentiated thyroid cancer cells [ 38 ], prostate cancer cells [ 39 ], lung cancer cells [ 40 ], breast cancer cells [ 41 ]and so on. This shows that it has broad prospects in cancer treatment.…”

Long non-coding RNA LINC01207 promotes cell proliferation and migration but suppresses apoptosis and autophagy in oral squamous cell carcinoma by the microRNA-1301-3p/lactate dehydrogenase isoform A axis

“…CCK-8 Kit (Beyotime, Shanghai, China) was used to assess cell viability as previously described [ 32 ]. After being transfected for 48 h, HSC-3 and HSC-4 cells (5000 cells per well) were seeded in 96-well plates.…”

“…CCK-8 Kit (Beyotime, Shanghai, China) was used to assess cell viability as previously described [32].…”

Saikosaponin D: review on the antitumour effects, toxicity and pharmacokinetics

“…However, human osteosarcoma 143B cells and MG-63 cells were not as sensitive to the cytotoxicity of SSD as U2 cells, only when the concentration is as high as 80 µM in vitro did SSD significantly induced proliferative inhibition, G0/G1-phase arrest and apoptosis via activating p53 and then regulating its downstream targets (Zhao et al. 2019 ).…”