Salbutamol pretreatment does not change eosinophil percentage and eosinophilic cationic protein concentration in hypertonic saline‐induced sputum in asthmatic subjects

Cianchetti, Silvana; Bacci, Elena; Ruocco, L; Bartoli, Ml; Carnevali, S; Dente, Fl; Franco, A Di; Giannini, D; Scuotri, L; Vagaggini, Barbara; Paggiaro, Pier Luigi

doi:10.1046/j.1365-2222.1999.00519.x

Cited by 20 publications

(22 citation statements)

References 22 publications

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“…2-4 puffs from a standard metered-dose inhaler, has generally been used for pretreatment. Pretreatment with high doses of salbutamol has not been universally effective in preventing hypertonic saline-induced bronchoconstriction [12,[15][16][17]. In addition, subsequent bronchospasm may be more severe or more difficult to reverse.…”

Section: Bronchodilator Pretreatmentmentioning

confidence: 99%

Sputum induction

Chanez¹,

Holz²,

Ind³

et al. 2002

Eur Respir J

125

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The aim of sputum induction is to collect an adequate sample of secretions from lower airways in subjects who do not produce sputum spontaneously in order to study the features of airway inflammation in asthma and other respiratory disorders. Inhalation of isotonic or hypertonic solutions administered by nebulisation has been demonstrated to induce a small amount of airway secretion that can be expectorated and analysed. The mechanisms by which this occurs are not known, but both direct and indirect mechanisms are likely to be involved. It is believed that the increased osmolarity of the airway lining fluid increases vascular permeability in the bronchial mucosa and induces production of mucus by submucosal glands. Animal studies have shown that hypertonic saline increases vascular permeability in the airways, and this effect can be modulated by capsaicin [1]. However, in vivo instillation of hypertonic solution into the airways of animals and humans induces an increase in the levels of several mediators but no rise in levels of albumin or other markers of increased vascular permeability [2,3]. Therefore, this hypothesis has not been confirmed, and only preliminary measurements of sputum osmolarity after induction have been reported with conflicting results [4]. Conversely, previous reports have demonstrated increased clearance of secretions from the airways in humans after administration of hypertonic saline aerosol [5], perhaps by facilitating the collection of small amounts of pre-existing airway secretions.Although sputum induction has been used extensively since the 1990s, few methodological studies have examined the influence of various technical factors on the feasibility and repeatability of sputum induction and collection. As with other techniques, the lack of a "gold standard" makes it hard to evaluate the influence of these technical factors on the adequacy and accuracy of sputum induction.The technique of sputum induction consists of inhaling an aerosol of saline (either normal or hypertonic) over different time-periods. Volunteers/ patients are instructed to expectorate into a container. Various issues must be considered with regard to this procedure: 1) facilities, equipment and personnel; 2) bronchodilator pretreatment; 3) pulmonary function monitoring; 4) concentration of saline solution and nebuliser output; 5) duration of inhalation; 6) expectoration technique; 7) spontaneous versus induced sputum; and 8) frequency of sputum induction. The influence of these factors on the feasibility of the technique must be evaluated in terms of the success rate, repeatability and accuracy of the results obtained.

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Section: Bronchodilator Pretreatmentmentioning

confidence: 99%

Sputum induction

Chanez¹,

Holz²,

Ind³

et al. 2002

Eur Respir J

125

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“…Therefore, a single dose of salbutamol 200 μg is recommended both before and after the measurement of FEV 1 for 10 min. Salbutamol pretreatment does not interfere the inflammatory cell percentage in induced sputum (Cianchetti et al 1999. Regarding the effects on soluble mediators in sputum supernatant, salbutamol has no effect on eosinophil cationic protein (ECP) levels, but tends to reduce histamine concentrations (Cianchetti et al 1999).…”

Section: Sputum Inductionmentioning

confidence: 99%

“…Salbutamol pretreatment does not interfere the inflammatory cell percentage in induced sputum (Cianchetti et al 1999. Regarding the effects on soluble mediators in sputum supernatant, salbutamol has no effect on eosinophil cationic protein (ECP) levels, but tends to reduce histamine concentrations (Cianchetti et al 1999). There has been no study for the effect of salbutamol on the levels of other soluble mediators (i.e., cytokines, albumin, and neutrophil elastase) or the expression of cell activation markers as detected by immunocytochemistry.…”

Section: Sputum Inductionmentioning

confidence: 99%

“…Salbutamol (200-400 μg or 2-4 puffs from a metered dose inhaler) can be used for pretreatment. Pretreatment with higher doses of salbutamol cannot provide additional advantage for the prevention of hypertonic saline-induced bronchoconstriction (Wong & Fahy 1997, Cianchetti, et al 1999, de la Fuente, et al 1998, Peleman, et al 1999), but may induce more severe subsequent bronchoconstriction. Therefore, a single dose of salbutamol 200 μg is recommended both before and after the measurement of FEV 1 for 10 min.…”

Section: Sputum Inductionmentioning

confidence: 99%

See 1 more Smart Citation

Application of Immunocytochemistry to Sputum Cells to Investigate Molecular Mechanisms of Airway Inflammation

Maneechotesuwan¹,

Wongkajornsilp²

2012

Applications of Immunocytochemistry

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“…En effet, il a été montré que l'expectoration précoce (les 5 premières minutes) contient plus de granulocytes alors que l'expectoration tardive s'enrichit en cellules mononucléées [6] . Les solutions salines hypertoniques pouvant induire un bronchospasme sévère, il est conseillé de pré-traiter le patient avec des ␤2-mimétiques à courte durée d'action avant l'induction [7,8] . De plus, lorsque le volume maximal expiré en 1 seconde (VEMS) de base est inférieur à 65 %, il est préférable d'utiliser une solution saline isotonique [9] .…”

Section: Technique D'induction Et D'analyse De L'expectoration Induiteunclassified

Intérêt de la mesure de l’inflammation en clinique dans l’asthme

Schleich¹,

Louis²

2012

EMC - Pneumologie

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Salbutamol pretreatment does not change eosinophil percentage and eosinophilic cationic protein concentration in hypertonic saline‐induced sputum in asthmatic subjects

Abstract: Salbutamol pretreatment reduces the severity of bronchoconstriction induced by HS inhalation without significantly affecting the percentages of inflammatory cells and the levels of soluble mediators in induced sputum.

Cited by 20 publications

References 22 publications

Sputum induction

Sputum induction

Application of Immunocytochemistry to Sputum Cells to Investigate Molecular Mechanisms of Airway Inflammation

Intérêt de la mesure de l’inflammation en clinique dans l’asthme

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