Salicylic acid and cysteine contribute to arbutin-induced alleviation of angular leaf spot disease development in cucumber

Kużniak, Elźbieta; Wielanek, Marzena; Chwatko, Grażyna; Głowacki, Rafał; Libik-Konieczny, Marta; Piątek, Milena; Gajewska, Ewa; Skłodowska, Maria

doi:10.1016/j.jplph.2015.03.017

Cited by 12 publications

(8 citation statements)

References 34 publications

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“…Also treatments of Arabidopsis with SA and MeJA induced the expression of another gene family member UGT73C5 involved in mycotoxin detoxification (Poppenberger et al, 2003). Increased UDP transcript in FOL inoculated tomato can be attributed to the role of arbutin in detoxification of fungal toxin or its antimicrobial effect (Kuzniak et al, 2015). The increased expression of this gene in treatment of MeJA and not SA represents its role in ISR.…”

Section: Discussionmentioning

confidence: 99%

Streptomyces Strains Induce Resistance to Fusarium oxysporum f. sp. lycopersici Race 3 in Tomato Through Different Molecular Mechanisms

et al. 2019

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Plant growth promoting rhizobacteria (PGPR) are potential natural alternatives to chemical fungicides in greenhouse production via inducing plant immune system against biotic stresses. In this research, 126 Streptomyces isolates were recovered from rhizosphere soils of 13 different commercial vegetable greenhouses in Iran. Streptomyces isolates were screened for in vitro Plant growth promoting (PGP) traits and ability to antagonize Fusarium oxysporum f. sp. lycopersici race 3 ( FOL ), the causal agent of Fusarium wilt of tomato (FWT). Six isolates with the highest antagonistic activity and at least three PGP traits were selected and compared with chemical fungicide Carbendazim ® in a greenhouse experiment. All bacterial treatments mitigated FWT disease symptoms like chlorosis, stunting and wilting at the same level or better than Carbendazim ® . Strains IC10 and Y28 increased shoot length and shoot fresh and dry weight compared to not inoculated control plants. Phenotypic characterization and 16S rRNA gene sequencing showed, strains IC10 and Y28 were closely related to S. enissocaesilis and S. rochei , respectively. The ability of the superior biocontrol strains to induce antioxidant enzymes activity and systemic resistance (ISR) was investigated. Increased activity of catalase (CAT) in plant treated with both strains as well as an increase in peroxidase (POX) activity in plants treated with Y28 pointed to a strain specific-induced systemic resistance (ss-ISR) in tomato against FOL . The differential induced expression of WRKY70 and ERF1 (two transcription factors involved in plant defense) and LOX and TPX by the analyzed Streptomyces strains, especially after inoculation with FOL , suggests that ss-ISR is triggered at the molecular level.

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Section: Discussionmentioning

confidence: 99%

Streptomyces Strains Induce Resistance to Fusarium oxysporum f. sp. lycopersici Race 3 in Tomato Through Different Molecular Mechanisms

et al. 2019

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“…The increase of ROS with SA treatment is related to the SA signalling because SA‐dependent responses rely on redox signals. It has been shown that SA cooperates with ROS and redox sensitive cellular components to induce hypersensitive response (HR) and SAR during the plant–pathogen/parasite interaction (Kuźniak et al , ; Kuźniak et al , ). These results suggest a SA‐mediated induction of plant defence by regulating ROS level in sunflower to O .…”

Section: Discussionmentioning

confidence: 99%

Seed treatment with salicylic acid invokes defence mechanism ofHelianthus annuusagainstOrobanche cumana

Yang

Ali

et al. 2016

Annals of Applied Biology

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The root holoparasitic angiosperm sunflower broomrape (Orobanche cumana) specifically affects sunflower (Helianthus annuus) growth and causes severe damage all over the world. This investigation was designed to examine the protective effects of salicylic acid (SA) treatment to the seeds of an O. cumana-susceptible cultivar of sunflower (TK0409). Sunflower seeds were pretreated with different concentrations (0, 0.5 and 1 mM) of SA and inoculated with O. cumana for 4 weeks. O. cumana infection resulted in reduction in plant biomass, endogenous SA level, and the expression of SA-related genes including pal, chs and NPR1. By contrast, O. cumana infection enhanced the production of reactive oxygen species, activities of antioxidant enzymes as well as contents of phenolics and lignin. Seed treatment with 1 mM SA increased sunflower biomass in terms of plant height, fresh weight and dry weight by 10%, 13% and 26%, respectively, via reducing the number and biomass of established O. cumana. The increase of hydrogen peroxide contents by 14% in the 1 mM SA treated sunflower plants appeared to be because of the inhibition of ascorbate peroxidase and catalase by exogenous SA. The enhanced expression of pathogenesis-related genes (PR3 and PR12, encoding chitinase and defensin, respectively) after 4 weeks of inoculation indicated that systemic acquired resistance was induced in the SA treated sunflower in which the level of endogenous SA was also elevated in a dose-dependent manner. The increased expression of a hypersensitive-responsive (HR) gene hsr indicated that the resistance of sunflowers might be associated with a hypersensitive reaction which was activated by exogenous SA treatment.(H 2 O 2 ) in O. cumana-infected sunflowers promote an oxidative burst during the interaction of sunflower and O. cumana . Rapid production of ROS is seen as an indicator of successful recognition of infection by plants, leading to the activation of the plants' defence mechanism (Torres et al., 2006;Torres, 2010). Accumulation of H 2 O 2 in peas infected by Orobanche crenata was observed by using both biochemical and cytochemical techniques (Pérez-de-Luque et al., 2006;Mabrouk et al., 2007). However, Mor et al. (2008) found that ROS detected at the interaction site on Arabidopsis thaliana infected by Orobanche aegyptiaca probably originated from the parasite, which was important for 408 Ann Appl Biol 169 (2016) 408-422

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“…However, induced resistance in cucumber leaves to powdery mildew has been found to be dependent on elevated activity of flavonoid pathway enzymes [ 32 ], suggesting potential for induced response as well. Increased expression of PAL, a key upstream enzyme for flavonoids, as well as salicylic acid and lignin biosynthesis, has been observed in a wide range of systems, including cucumber and melon, in response to treatment with pathogens or chemical inducers (e.g., [ 57 , 58 ]).…”

Section: Discussionmentioning

confidence: 99%

Exocarp Properties and Transcriptomic Analysis of Cucumber (Cucumis sativus) Fruit Expressing Age-Related Resistance to Phytophthora capsici

et al. 2015

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Very young cucumber (Cucumis sativus) fruit are highly susceptible to infection by the oomycete pathogen, Phytophthora capsici. As the fruit complete exponential growth, at approximately 10–12 days post pollination (dpp), they transition to resistance. The development of age-related resistance (ARR) is increasingly recognized as an important defense against pathogens, however, underlying mechanisms are largely unknown. Peel sections from cucumber fruit harvested at 8 dpp (susceptible) and 16 dpp (resistant) showed equivalent responses to inoculation as did whole fruit, indicating that the fruit surface plays an important role in defense against P. capsici. Exocarp from 16 dpp fruit had thicker cuticles, and methanolic extracts of peel tissue inhibited growth of P. capsici in vitro, suggesting physical or chemical components to the ARR. Transcripts specifically expressed in the peel vs. pericarp showed functional differentiation. Transcripts predominantly expressed in the peel were consistent with fruit surface associated functions including photosynthesis, cuticle production, response to the environment, and defense. Peel-specific transcripts that exhibited increased expression in 16 dpp fruit relative to 8 dpp fruit, were highly enriched (P<0.0001) for response to stress, signal transduction, and extracellular and transport functions. Specific transcripts included genes associated with potential physical barriers (i.e., cuticle), chemical defenses (flavonoid biosynthesis), oxidative stress, penetration defense, and molecular pattern (MAMP)-triggered or effector-triggered (R-gene mediated) pathways. The developmentally regulated changes in gene expression between peels from susceptible- and resistant- age fruits suggest programming for increased defense as the organ reaches full size.

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Salicylic acid and cysteine contribute to arbutin-induced alleviation of angular leaf spot disease development in cucumber

Cited by 12 publications

References 34 publications

Streptomyces Strains Induce Resistance to Fusarium oxysporum f. sp. lycopersici Race 3 in Tomato Through Different Molecular Mechanisms

Streptomyces Strains Induce Resistance to Fusarium oxysporum f. sp. lycopersici Race 3 in Tomato Through Different Molecular Mechanisms

Seed treatment with salicylic acid invokes defence mechanism ofHelianthus annuusagainstOrobanche cumana

Exocarp Properties and Transcriptomic Analysis of Cucumber (Cucumis sativus) Fruit Expressing Age-Related Resistance to Phytophthora capsici

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