Salicylic Acid Enhances Resistance to Fusarium oxysporum f. sp. phaseoli in Common Beans (Phaseolus vulgaris L.)

Xue, Ren; Wu, Jing; Wang, Lan Fen; Blair, Matthew W.; Wang, Xiao Ming; Ge, Wei; Zhu, Zheng; Wang, Shu Min

doi:10.1007/s00344-013-9376-y

Cited by 17 publications

(15 citation statements)

References 18 publications

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“…It was shown before in a number of reports that a reduced PAL gene transcript level correlates with decreased SA levels and increased plant susceptibility to pathogen infection (Huang et al, 2010). It is known that PAL and other genes of the phenylpropanoid pathway (e.g., 4CL, CHS ) are activated upon induction with both fungal and cell wall elicitors (Davis and Hahlbrock, 1987; Lawton and Lamb, 1987; Xue et al, 2014). In elicited flax up-regulation of PAL , cinnamoyl-CoA reductase ( CCR ), and cinnamyl alcohol dehydrogenase ( CAD ) was reported (Hano et al, 2006; Kostyn et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Methyl Salicylate Level Increase in Flax after Fusarium oxysporum Infection Is Associated with Phenylpropanoid Pathway Activation

et al. 2017

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Flax (Linum usitatissimum) is a crop plant valued for its oil and fiber. Unfortunately, large losses in cultivation of this plant are caused by fungal infections, with Fusarium oxysporum being one of its most dangerous pathogens. Among the plant's defense strategies, changes in the expression of genes of the shikimate/phenylpropanoid/benzoate pathway and thus in phenolic contents occur. Among the benzoates, salicylic acid, and its methylated form methyl salicylate play an important role in regulating plants' response to stress conditions. Upon treatment of flax plants with the fungus we found that methyl salicylate content increased (4.8-fold of the control) and the expression profiles of the analyzed genes suggest that it is produced most likely from cinnamic acid, through the β-oxidative route. At the same time activation of some genes involved in lignin and flavonoid biosynthesis was observed. We suggest that increased methyl salicylate biosynthesis during flax response to F. oxysporum infection may be associated with phenylpropanoid pathway activation.

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Section: Discussionmentioning

confidence: 99%

Methyl Salicylate Level Increase in Flax after Fusarium oxysporum Infection Is Associated with Phenylpropanoid Pathway Activation

et al. 2017

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“…The activity of two well-known defense enzymes, PAL and POX, were assayed following the method of Xue et al[ 21 ] with some modifications. Enzymatic activity of PAL was determined by the production of cinnamate, measured by the absorbance change at 290 nm with a UV-160 spectrophotometer (Puxi Corp., Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

“…In addition, two factors related to defense response including total hydrogen peroxide (H 2 O 2 ) content and superoxide anion (O 2 - ) production were also measured according to Xue et al[ 21 ] with the following modifications. For the H 2 O 2 assay, the reaction mixture was centrifuged and the absorbance of the supernatant was measured at 480 nm.…”

Section: Methodsmentioning

confidence: 99%

Differentially Expressed Genes in Resistant and Susceptible Common Bean (Phaseolus vulgaris L.) Genotypes in Response to Fusarium oxysporum f. sp. phaseoli

Xue

Zhu

et al. 2015

PLoS ONE

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Fusarium wilt of common bean (Phaseolus vulgaris L.), caused by Fusarium oxysporum Schlechtend.:Fr. f.sp. phaseoli (Fop), is one of the most important diseases of common beans worldwide. Few natural sources of resistance to Fop exist and provide only moderate or partial levels of protection. Despite the economic importance of the disease across multiple crops, only a few of Fop induced genes have been analyzed in legumes. Therefore, our goal was to identify transcriptionally regulated genes during an incompatible interaction between common bean and the Fop pathogen using the cDNA amplified fragment length polymorphism (cDNA-AFLP) technique. We generated a total of 8,730 transcript-derived fragments (TDFs) with 768 primer pairs based on the comparison of a moderately resistant and a susceptible genotype. In total, 423 TDFs (4.9%) displayed altered expression patterns after inoculation with Fop inoculum. We obtained full amplicon sequences for 122 selected TDFs, of which 98 were identified as annotated known genes in different functional categories based on their putative functions, 10 were predicted but non-annotated genes and 14 were not homologous to any known genes. The 98 TDFs encoding genes of known putative function were classified as related to metabolism (22), signal transduction (21), protein synthesis and processing (20), development and cytoskeletal organization (12), transport of proteins (7), gene expression and RNA metabolism (4), redox reactions (4), defense and stress responses (3), energy metabolism (3), and hormone responses (2). Based on the analyses of homology, 19 TDFs from different functional categories were chosen for expression analysis using quantitative RT-PCR. The genes found to be important here were implicated at various steps of pathogen infection and will allow a better understanding of the mechanisms of defense and resistance to Fop and similar pathogens. The differential response genes discovered here could also be used as molecular markers in association mapping or QTL analysis.

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“…; Xue et al . ). In addition, some in vitro tests showed that SA can act directly as an antimicrobial compound by inhibiting conidial germination and mycelial growth of fungi.…”

Section: Introductionmentioning

confidence: 97%

“…Many researchers have reported that exogenous application of SA at nontoxic concentration could enhance disease resistance and decrease spoilage in various fruits such as mangoes (Zeng et al 2006), sweet cherries , Chinese winter jujube (Cao et al 2013), peach Panahirad et al 2012) and citrus fruits (Zhou et al 2014). The mechanisms of disease resistance by SA in postharvest fruit included oxidative burst, production of antimicrobial phytoalexins and synthesis of pathogenesis-related (PR) proteins Asghari and Aghdam 2010;Cao et al 2013;Xue et al 2014). In addition, some in vitro tests showed that SA can act directly as an antimicrobial compound by inhibiting conidial germination and mycelial growth of fungi.…”

Section: Introductionmentioning

confidence: 99%

Defense Responses of Salicylic Acid in Mango Fruit Against Postharvest Anthracnose, Caused by Colletotrichum gloeosporioides and its Possible Mechanism

Ren

Chen

et al. 2016

Journal of Food Safety

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The effects and mechanisms of salicylic acid (SA) on defense response to Colletotrichum gloeosporioides from mango fruits were investigated by in vitro and in vivo test. In vitro experiment results showed that SA significantly reduced mycelial growth of C. gloeosporioides in a concentration‐dependent manner. SA effectively controlled anthracnose decay on inoculated mango fruit, as well as natural infection. Disease incidence and lesion diameter in SA‐treated fruit were significantly lower than those of the control fruit. SA treatment increased the activities of chitinase, β‐1,3‐glucanase, phenylalanine ammonia‐lyase and polyphenoloxidase, and the content of total phenolic compounds and lignin in mango fruit. Moreover, SA treatment effectively maintained fruit firmness by suppressing conversion of insoluble protopectin into water soluble pectin. And correlation analysis showed there is a higher negative correlation between fruit firmness and disease incidence. These findings suggest that the effect of SA on postharvest diseases was attributed to its direct antimicrobial activity and the elicitation of resistant responses, as well maintaining the firmness in mango fruit. Therefore, SA treatment is a promising measure for controlling postharvest anthracnose rot in mango. Practical Applications Induction of fruit resistance against pathogenic infection with biological or chemical elicitors has been thought to be a promising approach for controlling of postharvest diseases and reducing the use of synthetic fungicides. Anthracnose, caused by Colletotrichum gloeosporioides is the predominant postharvest disease in mango production that causes severe postharvest losses and fruit quality deterioration. SA is a natural plant substance involved in plant defense responses to biotic stresses. The present results from in vitro and in vivo experiment suggested that SA treatment could effectively inhibit mycelial growth of C. gloeosporioides, enhance resistance of mango fruit against the pathogen and reduce anthracnose rot, as well maintain fruit firmness. Hence, SA can be applied in mango storage and preservation.

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Salicylic Acid Enhances Resistance to Fusarium oxysporum f. sp. phaseoli in Common Beans (Phaseolus vulgaris L.)

Cited by 17 publications

References 18 publications

Methyl Salicylate Level Increase in Flax after Fusarium oxysporum Infection Is Associated with Phenylpropanoid Pathway Activation

Methyl Salicylate Level Increase in Flax after Fusarium oxysporum Infection Is Associated with Phenylpropanoid Pathway Activation

Differentially Expressed Genes in Resistant and Susceptible Common Bean (Phaseolus vulgaris L.) Genotypes in Response to Fusarium oxysporum f. sp. phaseoli

Defense Responses of Salicylic Acid in Mango Fruit Against Postharvest Anthracnose, Caused by Colletotrichum gloeosporioides and its Possible Mechanism

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