Salicylic acid-induced cytosolic acidification increases the accumulation of phenolic acids in Salvia miltiorrhiza cells

Li, Xiuhong; Guo, Hongbo; Qi, Yuexin; Liu, Hailong; Zhang, Xiaoru; Ma, Pengda; Liang, Zongsuo; Dong, Juane

doi:10.1007/s11240-016-1001-x

Cited by 31 publications

(16 citation statements)

References 33 publications

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“…Elicitors such as yeast elicitors, metal ions (Ag + , Cu 2+ , Zn 2+ , Co 2+ ), plant growth regulators (SA, ABA, ETH, MeJA, IAA, NAA, GA, 6-BA, TDZ), and other treatments (polyamines, ultraviolet-B radiation, H 2 O 2 ), induce the biosynthesis and accumulation of secondary metabolites (tanshinone and phenolic acid biosynthesis) in S. miltiorrhiza [ 11 , 13 ], especially plant hormones (MeJA, SA, ETH, ABA, GA) [ 62 – 64 ]. MAPK cascades have also been implicated in ABA, ETH, JA and SA signalling [ 17 , 25 , 29 , 33 , 36 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide characterization and expression profiling of MAPK cascade genes in Salvia miltiorrhiza reveals the function of SmMAPK3 and SmMAPK1 in secondary metabolism

et al. 2020

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Background The contribution of mitogen-activated protein kinase (MAPK) cascades to plant growth and development has been widely studied, but this knowledge has not yet been extended to the medicinal plant Salvia miltiorrhiza, which produces a number of pharmacologically active secondary metabolites. Results In this study, we performed a genome-wide survey and identified six MAPKKK kinases (MAPKKKKs), 83 MAPKK kinases (MAPKKKs), nine MAPK kinases (MAPKKs) and 18 MAPKs in the S. miltiorrhiza genome. Within each class of genes, a small number of subfamilies were recognized. A transcriptional analysis revealed differences in the genes’ behaviour with respect to both their site of transcription and their inducibility by elicitors and phytohormones. Two genes were identified as strong candidates for playing roles in phytohormone signalling. A gene-to-metabolite network was constructed based on correlation analysis, highlighting the likely involvement of two of the cascades in the synthesis of two key groups of pharmacologically active secondary metabolites: phenolic acids and tanshinones. Conclusion The data provide insight into the functional diversification and conservation of MAPK cascades in S. miltiorrhiza.

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Section: Discussionmentioning

confidence: 99%

Genome-wide characterization and expression profiling of MAPK cascade genes in Salvia miltiorrhiza reveals the function of SmMAPK3 and SmMAPK1 in secondary metabolism

et al. 2020

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“…Phenolic acids are a class of bioactive compounds in S. miltiorrhiza . Previous studies have shown that salicylic acid affects the expression of genes involved in phenolic acid biosynthesis and leads to the accumulation of these compounds in S. miltiorrhiza [ 7 , 9 ]. In this study, we found that more than 62% of the identified 562 SmPPRs responded to salicylic acid treatment.…”

Section: Resultsmentioning

confidence: 99%

“…However, the function of many PPRs is still unknown and there is a lack of knowledge about SmPPRs . Previous studies suggested that salicylic acid could inhibit the activity of plasma membrane H + -ATPase, could make cell generate oxidative stress, could promote the synthesis of phenolic acids, and up-regulate the expression of PAL , TAT , and RAS in S. miltiorrhiza cell culture [ 7 , 9 ]. Ag + could trigger a burst of reactive oxygen species (ROS) and yeast extract could improve the antioxidant defense of S. miltiorrhiza hairy roots [ 6 , 11 ].…”

Section: Discussionmentioning

confidence: 99%

“…Lipophilic diterpenoids such as tanshinone I, tanshinone IIA, tanshinone IIB, dihydrotanshnone I, and cryptotanshinone are diterpene quinines derived from the 2-C-methyl- d -erythritol 4-phosphate (MEP) pathway and/or the mevalonate (MVA) [ 4 , 5 ]. Due to great economic and medicinal value, the biosynthesis of bioactive components and its regulation mechanism in S. miltiorrhiza have attracted widespread interest [ 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ]. Many genes related to the biosynthetic pathways of tanshinones and phenolic acids have been identified [ 4 , 5 , 13 ] (see Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

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The Pentatricopeptide Repeat Gene Family in Salvia miltiorrhiza: Genome-Wide Characterization and Expression Analysis

Deng

et al. 2018

Molecules

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The pentatricopeptide repeat (PPR) gene family is one of the largest gene families in plants and plays important roles in posttranscriptional regulation. In this study, we combined whole genome sequencing and transcriptomes to systematically investigate PPRs in Salvia miltiorrhiza, which is a well-known material of traditional Chinese medicine and an emerging model system for medicinal plant studies. Among 562 identified SmPPRs, 299 belong to the P subfamily while the others belong to the PLS subfamily. The majority of SmPPRs have only one exon and are localized in the mitochondrion or chloroplast. As many as 546 SmPPRs were expressed in at least one tissue and exhibited differential expression patterns, which indicates they likely play a variety of functions in S. miltiorrhiza. Up to 349 SmPPRs were salicylic acid-responsive and 183 SmPPRs were yeast extract and Ag+-responsive, which indicates these genes might be involved in S. miltiorrhiza defense stresses and secondary metabolism. Furthermore, 23 salicylic acid-responsive SmPPRs were co-expressed with phenolic acid biosynthetic enzyme genes only while 16 yeast extract and Ag+-responsive SmPPRs were co-expressed with tanshinone biosynthetic enzyme genes only. Two SmPPRs were co-expressed with both phenolic acid and tanshinone biosynthetic enzyme genes. The results provide a useful platform for further investigating the roles of PPRs in S. miltiorrhiza.

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“…Salicylic acid is widely used as an elicitor to stimulate the synthesis of secondary metabolites [60]. The accumulation of nicotine and other related alkaloids in tobacco can also be affected by environmental factors and levels of plant hormones, including that of JA [61][62][63].…”

Section: Discussionmentioning

confidence: 99%

Salicylic Acid-Producing Endophytic Bacteria Increase Nicotine Accumulation and Resistance against Wildfire Disease in Tobacco Plants

Islam¹,

Ali

Choi

et al. 2019

Microorganisms

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Endophytic bacteria (EB) are both a novel source of bioactive compounds that confer phytopathogen resistance and inducers of secondary metabolites in host plants. Twenty-seven EB isolated from various parts of Metasequoia glyptostroboides, Ginkgo biloba, Taxus brevifolia, Pinus densiflora, Salix babylonica, and S. chaenomeloides could produce salicylic acid (SA). The highest producers were isolates EB-44 and EB-47, identified as Pseudomonas tremae and Curtobacterium herbarum, respectively. Nicotiana benthamiana grown from EB-44-soaked seeds exhibited a 2.3-fold higher endogenous SA concentration and increased resistance against P. syringae pv. tabaci, the causative agent of tobacco wildfire disease, than plants grown from water-soaked seeds. N benthamiana and N. tabacum grown from EB-44-treated seeds developed 33% and 54% disease lesions, respectively, when infected with P. syringae pv. tabaci, and showed increased height and weight, in addition to 4.6 and 1.4-fold increases in nicotine accumulation, respectively. The results suggest that SA-producing EB-44 can successfully colonize Nicotiana spp., leading to increased endogenous SA production and resistance to tobacco wildfire disease. The newly isolated EB can offer an efficient and eco-friendly solution for controlling wildfire disease and nicotine accumulation in Nicotiana, with additional application for other important crops to increase both productivity and the generation of bioactive compounds.

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Salicylic acid-induced cytosolic acidification increases the accumulation of phenolic acids in Salvia miltiorrhiza cells

Cited by 31 publications

References 33 publications

Genome-wide characterization and expression profiling of MAPK cascade genes in Salvia miltiorrhiza reveals the function of SmMAPK3 and SmMAPK1 in secondary metabolism

Genome-wide characterization and expression profiling of MAPK cascade genes in Salvia miltiorrhiza reveals the function of SmMAPK3 and SmMAPK1 in secondary metabolism

The Pentatricopeptide Repeat Gene Family in Salvia miltiorrhiza: Genome-Wide Characterization and Expression Analysis

Salicylic Acid-Producing Endophytic Bacteria Increase Nicotine Accumulation and Resistance against Wildfire Disease in Tobacco Plants

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