Salidroside protects against premature senescence induced by ultraviolet B irradiation in human dermal fibroblasts

Mao, Genxiang; Xing, Wen Min; Wen, Xiaohua; Jia, Binghao; Yang, Zhouxin; Wang, Y.-Z.; Jin, Xing-Ji; Wang, G.-F.; Yan, Jing

doi:10.1111/ics.12202

Cited by 30 publications

(28 citation statements)

References 46 publications

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“…Fibroblasts. Our previous studies indicated that the optimal dose range of SAL for its protection against stress-induced premature senescence (SIPS) in human diploid 2BS fibroblasts is 5-20 μM [21,22]. In this study, we first observed the effects of SAL on replicative lifespan and biomarkers related to cellular senescence in human 2BS fibroblasts.…”

Section: Sal Delays Replicative Senescence Of Human 2bsmentioning

confidence: 91%

“…Our previous studies have indicated that SAL showed a potent antiaging effect in an accelerated mouse aging model induced by Dgalactose [20]. It also protects human fibroblast 2BS cells from premature senescence induced by H 2 O 2 or UVB exposure in human diploid fibroblasts [21,22], identified as stress-induced premature senescence (SIPS), which is mainly caused by oxidative stress and subsequent DNA damage. Besides, we found that SAL delays the replicative senescence of human diploid fibroblasts 2BS cells, which may be related to its stimulatory role on mitochondrial biogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Salidroside Delays Cellular Senescence by Stimulating Mitochondrial Biogenesis Partly through a miR-22/SIRT-1 Pathway

Mao

Wang

et al. 2019

Oxidative Medicine and Cellular Longevity

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Calorie restriction (CR) is a nongenetic intervention with a robust effect on delaying aging in mammals and other organisms. A mild stimulation on mitochondrial biogenesis induced by CR seems to be an important action mode for its benefits. Here, we reported that a component isolated from Rhodiola rosea L., salidroside, delays replicative senescence in human fibroblasts, which is related to its stimulation on mitochondrial biogenesis by activating SIRT1 partly resulted from inhibition on miR-22. Salidroside increased the mitochondrial mass that accompanied an increment of the key regulators of mitochondrial biogenesis including PGC-1α, NRF-1, and TFAM and reversed the mitochondrial dysfunction in presenescent 50PD cells, showing a comparable effect to that of resveratrol. SIRT1 is involved in the inducement of mitochondrial biogenesis by salidroside. The declined expression of SIRT1 in 50PD cells compared with the young 30PD cells was prevented upon salidroside treatment. In addition, pretreatment of EX-527, a selective SIRT1 inhibitor, could block the increased mitochondrial mass and decreased ROS production induced by salidroside in 50PD cells, resulting in an accelerated cellular senescence. We further found that salidroside reversed the elevated miR-22 expression in presenescent cells according to a miRNA array analysis and a subsequent qPCR validation. Enforced miR-22 expression by using a Pre-miR-22 lentiviral construct induced the young fibroblasts (30PD) into a senescence state, accompanied with increased senescence-related molecules including p53, p21, p16, and decreased SIRT1 expression, a known target of miR-22. However, salidroside could partly impede the senescence progression induced by lenti-Pre-miR-22. Taken together, our data suggest that salidroside delays replicative senescence by stimulating mitochondrial biogenesis partly through a miR22/SIRT1 pathway, which enriches our current knowledge of a salidroside-mediated postpone senility effect and provides a new perspective on the antidecrepitude function of this naturally occurring compound in animals and humans.

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Section: Sal Delays Replicative Senescence Of Human 2bsmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Salidroside Delays Cellular Senescence by Stimulating Mitochondrial Biogenesis Partly through a miR-22/SIRT-1 Pathway

Mao

Wang

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Salidroside is a phenylethanoid found in Rhodiola rosea L. , a plant that grows in high-altitude regions and has long been used as traditional medicine in China and Eastern Europe due to its beneficial effects against multiple pathological processes such as cerebral and endothelial dysfunction, hepatic injury and osteoporosis (Mao et al 2015). The anti-photoaging properties of this compound were investigated by Mao and colleagues (2015) using UVB-irradiated NHDF.…”

Section: Natural Compounds Used Against Photoagingmentioning

confidence: 99%

“…Salidroside treatment prevented UVB-induced cell cycle arrest and decreased the expression of the senescence-associated factors p21, p53 and p16. In addition, salidroside was able to protect the cells from UVB-induced synthesis of MMP-1 and of the pro-inflammatory cytokines IL-6 and TNF-α (Mao et al 2015). More recently, Xiao-Ying and colleagues (2016) showed that salidroside treatment is also able to protect HaCaT keratinocytes against UVB-mediated decrease in cell viability in a dose-dependent manner.…”

Section: Natural Compounds Used Against Photoagingmentioning

confidence: 99%

Plant extracts and natural compounds used against UVB-induced photoaging

et al. 2017

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Skin is continuously exposed to a variety of environmental stresses, including ultraviolet (UV) radiation. UVB is an inherent component of sunlight that crosses the epidermis and reaches the upper dermis, leading to increased oxidative stress, activation of inflammatory response and accumulation of DNA damage among other effects. The increase in UVB radiation on earth due to the destruction of stratospheric ozone poses a major environmental threat to the skin, increasing the risk of damage with long-term consequences, such as photoaging and photocarcinogenesis. Extracts from plants and natural compounds have been historically used in traditional medicine in the form of teas and ointments but the effect of most of these compounds has yet to be verified. Regarding the increasing concern of the population with issues related to quality of life and appearance, the cosmetic market for anti-aging and photoprotective products based on natural compounds is continuously growing, and there is increasing requirement of expansion on research in this field. In this review we summarized the most current and relevant information concerning plant extracts and natural compounds that are able to protect or mitigate the deleterious effects caused by photoaging in different experimental models.

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“…Salidroside has primarily been used as a brain tonic, a roborant or headache relief agent (8,12). Recently, salidroside has been studied in experimental animals for its protective effects against hypoxia, cold, radiation and heavy physical exercise (11).…”

Section: Introductionmentioning

confidence: 99%

Salidroside induces apoptosis in human ovarian cancer SKOV3 and A2780 cells through the p53 signaling pathway

Zhao

et al. 2018

Oncol Lett

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Abstract. Salidroside is one of the most potent compounds extracted from the plant Rhodiola rosea, and its cardiovascular protective effects have been studied extensively. However, the role of salidroside in human ovarian carcinoma remains unknown. The aim of the current study was to investigate the effects of salidroside on the proliferation and apoptosis of SKOV3 and A2780 cells using MTT assay and acridine orange/ethidium bromide staining. Salidroside activated caspase-3 and upregulated the levels of apoptosis-inducing factor, Bcl-2-associated X and Bcl-2-associated death promoter (Bad) proteins. Furthermore, salidroside downregulated the levels of Bcl-2, p-Bad and X-linked inhibitor of apoptosis proteins. Salidroside activated the caspase-dependent pathway in SKOV3 and A2780 cells, upregulating p53, p21Cip1/Waf1 and p16 INK4a . These results suggest that the p53/p21 Cip1/Waf1 /p16INK4a pathway may serve a key function in salidroside-mediated effects on SKOV3 and A2780 cells. The current findings indicate that salidroside may be a promising novel drug candidate for ovarian cancer therapy.

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Salidroside protects against premature senescence induced by ultraviolet B irradiation in human dermal fibroblasts

Cited by 30 publications

References 46 publications

Salidroside Delays Cellular Senescence by Stimulating Mitochondrial Biogenesis Partly through a miR-22/SIRT-1 Pathway

Salidroside Delays Cellular Senescence by Stimulating Mitochondrial Biogenesis Partly through a miR-22/SIRT-1 Pathway

Plant extracts and natural compounds used against UVB-induced photoaging

Salidroside induces apoptosis in human ovarian cancer SKOV3 and A2780 cells through the p53 signaling pathway

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