Salinirubellus salinus gen. nov., sp. nov., isolated from a marine solar saltern

Abstract

A halophilic archaeal strain, designated ZS-35-S2, was isolated from Zhoushan marine solar saltern in Zhejiang Province, China. Cells were pleomorphic, Gram-stain-negative and formed red-pigmented colonies on agar plates. The cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). Strain ZS-35-S2 was able to grow at 25-50 °C (optimum, 37 °C), with 1.4-4.8 M NaCl (optimum, 2.1 M), with 0-1.0 M MgCl2 (optimum, 0.1 M) and at pH 5.0-9.5 (optimum, pH 7.5). Phylogenetic tree reconstructions based on 16S rRNA genes and rpoB' genes revealed that strain ZS-35-S2 was distinct from the related genera Halomarina, Natronomonas, Halorientalis, Salinirubrum and Halobaculum of the order Halobacteriales. The major polar lipids of the strain were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and three unidentified glycolipids. The two major glycolipids were chromatographically identical to S-DGD-1 and DGD-1, respectively. The DNA G+C content of strain ZS-35-S2 is 67.0 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain ZS-35-S2 (=CGMCC 1.12551=JCM 30036) represents a novel species of a new genus within the order Halobacteriales, for which the name Salinirubellus salinus gen. nov., sp. nov. is proposed.

Cultivation of halophilic archaea (class Halobacteria) from thalassohaline and athalassohaline environments

“…Inspired by the cultivation and isolation of Haloquadratum walsbyi, a defined medium (NOM) with pyruvate as carbon source was used to recover diverse halophilic archaea from marine solar salterns and from salted Laminaria (a brown alga) (Cui et al 2010). Since then, cultivation studies using this medium, as well as those used by other laboratories, have resulted in the isolation and description of 14 new genera: Halobellus, Halogranum, Halohasta, Halolamina, Halopelagius, Halorientalis, Halorubellus, Halorussus, Salinarchaeum, Salinibaculum, Salinigranum, Salinirubellus, Salinirubrum, and Salinirussus were described (Amoozegar et al 2017;Cui et al 2017;Han and Cui 2020;Hou et al 2018). Like Halorubrum lacusprofundi, Halohasta litchfieldiae (strain tADL) was isolated from the Antarctic hypersaline waters of Deep Lake, but it was not readily cultivable on standard media, and isolation used the liquid end-point dilution method with DBCM2 medium (Mou et al 2012).…”

Salinigranum halophilum sp. nov., isolated from marine solar salterns

“…The cultivable halophilic archaea from these hypersaline environments are often related to Haloarcula , Halobacterium , Halobellus , Haloferax , Halogeometricum , Halolamina , Halomicrobium , Halonotius , Haloplanus , Halorientalis , Halorubrum , Haloterrigena , Halovivax , Natronomonas and Natronoarchaeum [2]. Halogranum , Halopelagius , Halorubellus , Halorussus [3–6], Salinigranum [7], Salinirubellus [8], Salinirubrum [9] and Salinirussus [10] have also been cultivated from brine or sediment samples of marine solar salterns. However, due to the lack of interspecific diversity, these taxa are currently classified based on only a single isolate [11].…”

Kinetics of Halophilic Enzymes

“…Root nodules of Sesbania cannabina grown in a high-salt and alkaline environment [120] Salinicola tamaricis F01 T Leaves of Tamarix chinensis [121] Salinirubellus salinus ZS-35-S2 T Marine solar saltern [122] Borderline extreme halophile 2.5-4.0 Aliifodinibius halophilus 2W32 T Marine solar saltern [123] Desulfosalsimonas propionicica PropA T…”