Salinity and growth effects on dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) cell quotas of Skeletonema costatum, Phaeocystis globosa and Heterocapsa triquetra

Speeckaert, Gaëlle; Borges, Alberto; Gypens, Nathalie

doi:10.1016/j.ecss.2019.106275

Cited by 15 publications

(16 citation statements)

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“…In reaction to the stress generated by the salinity, P.antarctica could have increased its DSC cell contents. These results are similar to those observed for P.globosa by Speeckaert et al (2019) and could suggest oxidative stress in the cell. Indeed, as suggested by Liu et al (2012) in the halophile green algae Dunaliella salina, hypo-osmotic stress at S20 could induce the accumulation in the chloroplast of reactive oxygen species (ROS) such as H2O2 or the hydroxyl radical • OH.…”

Section: Discussionsupporting

confidence: 90%

Dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) cell quotas variations arising from sea ice shifts of salinity and temperature in the Prymnesiophyceae Phaeocystis antarctica

et al. 2020

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Environmental contextDimethylsulfoniopropionate and dimethylsulfoxide could have a climatic influence especially in the polar areas. We investigate the effect of sea ice salinity and temperature on the production of these two sulfur metabolites by a polar microalga, and suggest their potential roles of osmoregulator and cryoprotectant. These results bring new information about the sulfur cycle in sea ice that is useful for climate models. AbstractThe Southern Ocean, which includes the seasonal ice zone (SIZ), is a source of large sea-air fluxes of dimethylsulfide (DMS), a climate active gas involved in Earth cooling processes. In this area, the prymnesiophyte Phaeocystis antarctica (P. antarctica) is one of the main producers of dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO), two metabolites that are precursors of DMS. These algae are also present in sea ice and contribute substantially to the high DMSP and DMSO concentrations observed in this habitat. DMSP and DMSO production in sea ice by P. antarctica is proposed to be promoted by its ability to live in extreme environmental conditions. We designed cell culture experiments to test that hypothesis, focusing on the impact of shifts of temperature and salinity on the DMSP and DMSO cell quotas. Our experiments show an increase in DMSP and DMSO cell quotas following shifts in salinity (34 to 75, at 4°C), which suggests a potential osmoregulator function for both DMSP and DMSO. Stronger salinity shifts (up to 100) directly impact cell growth and induce a crash of the cultures. Combining the salinity (34 to 75) and temperature (4°C to –2.3°C) shifts induces higher increases of DMSP and DMSO cell quotas that also suggests an implication of both metabolites in a cryoprotectant system. Experimental cell quotas (including diatom Fragilariopsis cylindrus quotas from a previous study) are then used to reconstruct DMSP and DMSO profiles in sea ice based on the biomass and taxonomy. Finally, the complexity of the transposition of rates obtained in the experimental domain to the real world is discussed.

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Section: Discussionsupporting

confidence: 90%

Dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) cell quotas variations arising from sea ice shifts of salinity and temperature in the Prymnesiophyceae Phaeocystis antarctica

et al. 2020

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“…I0; 100 μmol photons m −2 s −1 and 15 • C) were found to differ between species investigated, with the diatom possessing much less DMSP:Chl-a than the dinoflagellate and the Prymnesiophyceae. DMSP:Chl-a ratios measured in our cultures also agreed with previous studies conducted on H. triquetra (122.7 ± 27.7 mmolS:g Chl-a in average) and P. globosa (82.3 ± 11.5 mmolS:g Chl-a), maintained in similar environmental conditions (Keller et al, 1989;Niki et al, 2000;Speeckaert et al, 2018Speeckaert et al, , 2019Stefels et al 2007). For S. costatum, values were similar to those of Speeckaert et al (2018), who applied the same methodology on the same strain, but differed from other studies reporting lower DMSP:Chla ratio (4.5 to 11.8 mmolS:g Chl-a, in average; Keller and Korjeff-Bellows 1996;Sunda et al, 2007;Stefels et al, 2007;Spielmeyer et al, 2011;Speeckaert et al, 2019).…”

Section: Dmsp P and Dmso P Contents Vary Among Phytoplankton Species Investigatedsupporting

confidence: 92%

“…DMSP:Chl-a ratios measured in our cultures also agreed with previous studies conducted on H. triquetra (122.7 ± 27.7 mmolS:g Chl-a in average) and P. globosa (82.3 ± 11.5 mmolS:g Chl-a), maintained in similar environmental conditions (Keller et al, 1989;Niki et al, 2000;Speeckaert et al, 2018Speeckaert et al, , 2019Stefels et al 2007). For S. costatum, values were similar to those of Speeckaert et al (2018), who applied the same methodology on the same strain, but differed from other studies reporting lower DMSP:Chla ratio (4.5 to 11.8 mmolS:g Chl-a, in average; Keller and Korjeff-Bellows 1996;Sunda et al, 2007;Stefels et al, 2007;Spielmeyer et al, 2011;Speeckaert et al, 2019). The DMSO p -to-chlorophyll-a (DMSO p :Chl-a) ratios measured in this study were a bit higher than values reported in the literature, with 0.2 ± 0.2 for the diatoms, 1.5 ± 0.4 for the Prymnesiophyceae and 3.9 ± 4.3 mmolS:g Chl-a for the dinoflagellates (Simó et al, 1998;Hatton and Wilson, 2007;Bucciarelli et al, 2013;Speeckaert et al, 2019).…”

Section: Dmsp P and Dmso P Contents Vary Among Phytoplankton Species Investigatedsupporting

confidence: 92%

Response of dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) cell quotas to oxidative stress in three phytoplankton species

Royer

Gypens

Cardol

et al. 2021

Journal of Plankton Research

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Several phytoplankton species produce the metabolites dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) but their intracellular roles need to be better understood. To improve the understanding of the DMSP antioxidant function suggested by Sunda et al. (2002), we exposed the diatom Skeletonema costatum, the Prymnesiophyceae Phaeocystis globosa and the dinoflagellate Heterocapsa triquetra to experimental treatments known to cause potential oxidative stress (high light intensities (HL); HL with 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU); menadione sodium bisulfite (MSB)). DMSP and DMSO concentrations decreased after 6 h in all treatments indicating an interaction with Reactive Oxygen Species (ROS) produced. DMSP and DMSO-to-cell ratios in control conditions were higher for H. triquetra, while being unable to grow under HL. DMSP and DMSO-to-cell carbon were the highest for P. globosa, while the other species had similar values. During long-term treatment, these ratios were not increased in high-light grown cells of P. globosa and S. costatum. Overall, this illustrates that (1) the DMSP- and DMSO-to-cell or carbon seems to be not indicative of the capability of the species to tolerate an oxidative stress, (2) these molecules could react with ROS and lower their cellular concentration, but no clues demonstrated that these molecules are part of the antioxidant response of the cell.

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“…Moreover, its low molecular weight allows rapid entry through the cell membrane, in addition to modulating the stability of the phospholipid bilayer. Electrostatic interactions of DMSO with phospholipids have been suggested, appearing to be critical for membrane cryoprotection [ 81 , 82 ].…”

Section: Plant Vitrification Solutionmentioning

confidence: 99%

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Roque‐Borda

Kulus

Souza

et al. 2021

IJMS

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Numerous environmental and endogenous factors affect the level of genetic diversity in natural populations. Genetic variability is the cornerstone of evolution and adaptation of species. However, currently, more and more plant species and local varieties (landraces) are on the brink of extinction due to anthropopression and climate change. Their preservation is imperative for the sake of future breeding programs. Gene banks have been created worldwide to conserve different plant species of cultural and economic importance. Many of them apply cryopreservation, a conservation method in which ultra-low temperatures (−135 °C to −196 °C) are used for long-term storage of tissue samples, with little risk of variation occurrence. Cells can be successfully cryopreserved in liquid nitrogen (LN) when the adverse effect of ice crystal formation and growth is mitigated by the removal of water and the formation of the so-called biological glass (vitrification). This state can be achieved in several ways. The involvement of key cold-regulated genes and proteins in the acquisition of cold tolerance in plant tissues may additionally improve the survival of LN-stored explants. The present review explains the importance of cryostorage in agronomy and presents an overview of the recent works accomplished with this strategy. The most widely used cryopreservation techniques, classic and modern cryoprotective agents, and some protocols applied in crops are considered to understand which parameters provide the establishment of high quality and broadly applicable cryopreservation. Attention is also focused on the issues of genetic integrity and functional genomics in plant cryobiology.

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Salinity and growth effects on dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) cell quotas of Skeletonema costatum, Phaeocystis globosa and Heterocapsa triquetra

Abstract: Salinity and growth effects on dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) cell quotas of Skeletonema costatum, Phaeocystis globosa and Heterocapsa triquetra

Cited by 15 publications

References 80 publications

Dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) cell quotas variations arising from sea ice shifts of salinity and temperature in the Prymnesiophyceae Phaeocystis antarctica

Dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) cell quotas variations arising from sea ice shifts of salinity and temperature in the Prymnesiophyceae Phaeocystis antarctica

Response of dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) cell quotas to oxidative stress in three phytoplankton species

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

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