Salinity-induced oxidative stress-mediated change in fatty acids composition of cyanobacterium Synechococcus sp. PCC7942

Abstract

The present study was undertaken to examine the salinity stress-induced physiological and biochemical alterations in the cyanobacterium Synechococcus sp. PCC 7942. Cyanobacterial cultures supplemented with different concentrations of NaCl were evaluated for growth, carbohydrate, total lipid, ROS generation, and stress biomarkers to evaluate the ROS-mediated lipid production in Synechococcus 7942. Salt concentration of 500 mM induced a five-and threefold increase in the production of carbohydrates and lipids, respectively. The fatty acids composition in terms of total quantity and oleic acid content of the investigated species was also improved as the salinity level increased from 0 to 500 mM NaCl. The data showed maximum MUFA production at 10 mM NaCl with dominance of palmitoleic acid (88.3%) and oleic acid (0.31%), whereas PUFA was found to be maximally produced at 250 mM NaCl with dominance of linoleic acid. Salt stress enhanced the accumulation of carbohydrate and total lipids and antioxidative enzymes, and modulates the fatty acids and hydrocarbon composition of cyanobacterium. Production of fatty acid and hydrocarbon under saline conditions indicates that salinity can be used as a factor to modulate the biochemical pathways of cyanobacteria toward efficient biofuel production.

The endogenous L-cysteine desulfhydrase and hydrogen sulfide participate in supplemented phosphorus-induced tolerance to salinity stress in maize (Zeamays) plants

“…sodium (Na + ) and chloride (Cl − ), and deficiency of mineral elements (Isayenkov and Maathuis, 2019). Additionally, salinity leads to oxidative stress in plants because of overaccumulation of reactive oxygen species (Verma et al, 2019).…”

Improved Salt Tolerance and Metabolomics Analysis of Synechococcus elongatus UTEX 2973 by Overexpressing Mrp Antiporters

“…Furthermore, the percentage of C16:1 in strain JY13 with salt stress was 28.5% lower than that in strain JY00 with salt stress; the percentage of C18:1 in strain JY13 with salt stress was 1.53-fold higher than that in strain JY00 with salt stress. A previous study also showed that the content of C16:1 had significantly decreased by 29.1%, meanwhile, C18:1 increased ∼2-fold under 0.5 M NaCl conditions in S. elongatus PCC 7942 (Verma et al, 2018). However, the mechanism of conversion between C18:1 and C16:1 under salt stress is still unclear and further research should be performed in the near future.…”

“…In addition, the carbohydrate content of strain JY13 under salt stress was 10.2% higher than that of strain JY00 under salt stress, consistent with the changes of sucrose and glycogen detected by metabolomics analysis. Previous study revealed that the contents of carbohydrates increased ∼5-fold in S. elongates PCC 7942 under 0.5 M NaCl conditions compared to control (Verma et al, 2018). Thus, it's reasonable to suppose that the increased carbohydrates contents might be used as an adaptive strategy of cyanobacteria under saline conditions.…”

An appraisal of the variable response of microalgal lipids to culture salinity

“…The cyanobacterium Synechococcus sp. 95 and some strains of Chlorella, [96][97][98] Chlorococcum 97 and Chlamydomonas. 105 It is important to note that in many studies with freshwater species, growth was severely restricted by the increased salinity, a fact that indirectly influenced lipid accumulation.…”