Saliva: a convenient source of DNA for analysis of bi-allelic polymorphisms of Fcγ receptor IIA (CD32) and Fcγ receptor IIIB (CD16)

Schie, R. C. A. A. Van; Wilson, Mark

doi:10.1016/s0022-1759(97)00132-4

Cited by 34 publications

(28 citation statements)

References 33 publications

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“…As the first research of salivary DNA in Chinese twin children, we found no statistical significance of salivary DNA in both genders and age groups, which consists with those reports in the adults or between adults and infants (Freeman et al, 1997;Le Marchand et al, 2001;van Schie & Wilson, 1997). The decrease of salivary DNA yield with age groups may be affected by some reasons such as exfoliated velocity of oral epithelium and need to be explored in the future.…”

Section: Figuresupporting

confidence: 49%

“…Each child was asked to rinse his or her mouth with tap water and rest for about 5 minutes, then provide three milliliters of whole saliva in a 15-ml collecting tube noted with their name and collection time. Since the saliva samples would be used for hormone tests, we added nothing during transport and storage as previous description (Etter et al, 2005;Ng et al, 2004;Terasaki et al, 1998;van Schie & Wilson, 1997). Saliva samples collected at schools were transferred as soon as possible, and kept cold with ice by vacuum flask during transport, then frozen below -20 ºC and stored at -80 ºC until DNA extraction.…”

Section: Saliva Collection and Dna Extractionmentioning

confidence: 99%

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An Application of Salivary DNA in Twin Research of Chinese Children

Zhang

2008

Twin Res Hum Genet

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S ince saliva collection is noninvasive, painless and inexpensive, it may become an alternative to obtain genomic DNA, which is critical to evaluate zygosity and the role of genetic factors in twin research. This study provided a rough description of salivary DNA in Chinese twin children, and presented the DNA yield and quality extracted from saliva in a large-scale children sample, which supplied an example for saliva sample using in genetic epidemiology. Three milliliters of saliva was collected from 356 twin children aged 6 to 15, and DNA was extracted by a com mercial DNA isolation kit. The DNA yield and purity was determined by spectrophotometry at 260nm and 280nm. The zygosity determination of the same-sex twins and the assay of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism indicated the quality of salivary DNA. The amount of extracted DNA from three milliliters of saliva was about 34.91µg (2.20 ~ 122.04µg), average OD 260/280 values was 1.84. Saliva DNA is a reliable sample for the determination of twins' zygosity. We conclude that saliva may be a feasible and reliable source of DNA for genetic epidemiology studies, especially for twin research.

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Section: Figuresupporting

confidence: 49%

Section: Saliva Collection and Dna Extractionmentioning

confidence: 99%

An Application of Salivary DNA in Twin Research of Chinese Children

Zhang

2008

Twin Res Hum Genet

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“…On the other hand, there was no correlation between the PL in the blood and that in the oral fluid cells. In fact, the amount of cellular DNA that could be extracted from a standard volume of oral fluid varies widely between individuals and in the same subject over time (28). Accordingly, its use for PL follow-up might not be appropriate.…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of Mediterranean Visceral Leishmaniasis by Detection of Leishmania Antibodies and Leishmania DNA in Oral Fluid Samples Collected Using an Oracol Device

et al. 2011

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Current methods for diagnosis of visceral leishmaniasis (VL) require invasive sampling procedures such as visceral aspiration and/or blood drawing. The use of diagnostic tests using oral fluid, which is easier to collect, would be more simple and practical for VL diagnosis, especially under field conditions. Oral fluids from 37 VL patients and 40 healthy controls were collected using Oracol devices. Blood samples and oral fluid specimens from both groups were analyzed by recombinant protein K39 (rK39) enzyme-linked immunosorbent assay and quantitative real-time PCR. Detection of antibodies in the oral fluid had a sensitivity of 100% and a specificity of 97.5%. Antibody levels measured in serum and oral fluid showed a significant positive correlation ( ‫؍‬ 0.655 and P ‫؍‬ 0.01). Detection of Leishmania DNA in oral fluid had a sensitivity of 94.6% and a specificity of 90%. The median parasite load estimated in blood was 133 parasites/ml (interquartile range [IR], 10 to 1,048), whereas that in oral fluid specimens was 3 parasites/ml (IR, 0.41 to 92). However, there was no significant linear relationship between parasite loads assessed in the two biological samples ( ‫؍‬ 0.31 and P ‫؍‬ 0.06). VL diagnosis based on specific antibody detection and Leishmania DNA identification using oral fluid samples was equivalent in accuracy to that using blood and therefore is promising for clinical use.

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“…The majority of techniques reported to date have employed genomic DNA from peripheral blood in the performance of such analyses. We recently reported that DNA isolated from saliva is a satisfactory alternative to DNA from blood in PCR-based analyses of Fc␥RIIA and Fc␥RIIIB genotype (43). To date, however, neither we nor other groups have employed salivary DNA to define CD32 and/or CD16 genotype in various ethnic groups.…”

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confidence: 99%

Evaluation of Human FcγRIIA (CD32) and FcγRIIIB (CD16) Polymorphisms in Caucasians and African-Americans Using Salivary DNA

Schie

Wilson²

2000

Clin Diagn Lab Immunol

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Two classes of low-affinity receptors for the Fc region of immunoglobulin G (IgG) (Fc␥R) are constitutively expressed on resting human neutrophils. These receptors, termed Fc␥RIIa (CD32) and Fc␥RIIIb (CD16), display biallelic polymorphisms which have functional consequences with respect to binding and/or ingestion of targets opsonized by human IgG subclass antibodies. The H131-R131 polymorphism of CD32 influences binding of human IgG2 and, to a lesser extent, human IgG3 to neutrophils. The neutrophil antigen (NA1-NA2) polymorphism of CD16 influences the efficiency of phagocytosis of bacteria opsonized by human IgG1 and IgG3. These polymorphisms may influence host susceptibility to certain infectious and/or autoimmune diseases, prompting interest in the development of facile methods for determination of CD32 and CD16 genotype in various clinical settings. We previously reported that genomic DNA from saliva is a suitable alternative to DNA from blood in PCR-based analyses of CD32 and CD16 polymorphisms. In the present study, we utilized for the first time this salivary DNA-based methodology to define CD32 and CD16 genotypes in 271 Caucasian and 118 African-American subjects and to investigate possible linkage disequilibrium between certain CD32 and CD16 genotypes in these two ethnic groups. H131 and R131 gene frequencies were 0.45 and 0.55, respectively, among Caucasians and 0.59 among African-Americans. NA1 and NA2 gene frequencies were 0.38 and 0.62 among Caucasians and 0.39 and 0.61 among African-Americans. Since Fc␥RIIa and Fc␥RIIIb synergize in triggering neutrophils, we also assessed the frequency of different CD32 and CD16 genotype combinations in these two groups. In both groups, the R/R131-NA2/NA2 genotype combination was more common than the H/H131-NA1/NA1 combination (threefold for Caucasians versus sevenfold for AfricanAmericans). Whether individuals with the combined R/R131-NA2/NA2 genotype are at greater risk for development of infectious and/or autoimmune diseases requires further investigation, which can be conveniently performed using DNA from saliva rather than blood.

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Saliva: a convenient source of DNA for analysis of bi-allelic polymorphisms of Fcγ receptor IIA (CD32) and Fcγ receptor IIIB (CD16)

Cited by 34 publications

References 33 publications

An Application of Salivary DNA in Twin Research of Chinese Children

An Application of Salivary DNA in Twin Research of Chinese Children

Diagnosis of Mediterranean Visceral Leishmaniasis by Detection of Leishmania Antibodies and Leishmania DNA in Oral Fluid Samples Collected Using an Oracol Device

Evaluation of Human FcγRIIA (CD32) and FcγRIIIB (CD16) Polymorphisms in Caucasians and African-Americans Using Salivary DNA

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