Salivary alpha-amylase as a stress biomarker in diseased dogs

Hong, Hwa Ran; Oh, Ye In; Kim, Young Jun; Seo, Kyoung Won

doi:10.4142/jvs.2019.20.e46

Cited by 12 publications

(12 citation statements)

References 11 publications

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“…Therefore, a work activity characterized by greater effort led to lower levels of α-amylase; these results were contrary to our expectation. Indeed, most recent literature data relate increased levels of α-amylase to various stress factors, such as cardiovascular risk [ 32 ] and anxiety [ 33 , 34 , 35 ]. These contrasting results could be partly explained by the time of collection; in fact, the morning collection of the salivary sample overlaps as a time span with the negative post-awakening peak.…”

Section: Discussionmentioning

confidence: 99%

Salivary Biomarkers and Work-Related Stress in Night Shift Workers

Briguglio

Teodoro

Italia

et al. 2021

IJERPH

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Work organization, such as shifts and night work, can interfere with the perception of work-related stress and therefore on the development of pathological conditions. Night shift work, particularly, can have a negative impact on workers’ wellbeing by interfering with the biological sphere. The aim of this study is to evaluate the associations between work activities, shift work effects and stress-related responses in 106 dock workers enrolled in southeast Italy. Dock workers’ tasks consist of complex activities that seemed to affect more sleep quality than work-related stress. An analysis of salivary biomarkers such as cortisol, α-amylase, melatonin and lysozyme was performed along with validated psycho-diagnostic questionnaires. Alpha-amylase showed a significant negative correlation with the effort/reward imbalance score; thus, the measurement of salivary α-amylase is proposed as a sensitive and non-invasive biomarker of work-related stress. This study may provide new insights into developing strategies for the management of night shift work. Salivary biomarkers should be further investigated in the future in order to develop simple and effective tools for the early diagnosis of work-related stress or its outcomes.

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Section: Discussionmentioning

confidence: 99%

Salivary Biomarkers and Work-Related Stress in Night Shift Workers

Briguglio

Teodoro

Italia

et al. 2021

IJERPH

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“…The sAA is another representative of SAM activation, and has been investigated in dogs. The activity of sAA increased in dogs with various diseases compared to healthy dogs [28]. Salivary immunoglobulin A (sIgA) is a proven stress biomarker in human medicine [40].…”

Section: Discussionmentioning

confidence: 99%

“…Saliva analysis has been widely used in recent stress studies because it is less invasive than blood collection and causes less stress in patients [19,27]. Similarly, various salivary biomarkers, such as cortisol and salivary alpha amylase (sAA), have been investigated in veterinary medicine [19,28]. Specifically, methods for the quantification of AVP in saliva have been validated in domestic dogs.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of salivary vasopressin as an acute stress biomarker in healthy dogs with stress due to noise and environmental challenges

Jeong

Song

et al. 2020

BMC Vet Res

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Background Stress is associated with various detrimental changes in physiological health that affect an animal’s quality of life. The hypothalamus-pituitary-adrenal (HPA) axis and the sympathetic-adreno-medullar (SAM) axis are two main physiological pathways that constitute the stress response of an organism. Arginine vasopressin (AVP) is a mediator of the HPA axis and is known to be related to social behaviours and stress. The serum concentration of AVP is higher in more aggressive dogs and humans with post-traumatic stress disorder. Salivary biomarker analysis is a non-invasive method to assess stress. The purpose of this study was to evaluate the possibility of using salivary AVP as an acute stress biomarker in dogs. Salivary AVP concentration was measured before and after exposure to all relevant environmental stimuli (i.e. car trip to the lab, physical examination by the veterinarian, and sampling procedure,) and then after 30 min of vacuum noise exposure. Behavioural assessments, physiologic parameter assessments, and serum cortisol analysis were conducted in combination. Statistical analysis was conducted separately in the total study population, the less stressed group, and the more stressed group, respectively. Results Based on stress behaviour analysis scores, 28 dogs were classified into less or more stressed groups. All four physiologic parameters (blood pressure, body temperature, heart rate, and respiratory rate) were significantly increased after noise and environmental challenges, in the more stressed group. Serum cortisol did not show any significant change. Salivary AVP significantly decreased after noise and environmental stimulation in the more stressed group but not in the less stressed group. Salivary AVP and blood pressure changes were negatively correlated in the more stressed group. Conclusion Salivary AVP may be a potential acute stress biomarker in dogs.

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“…Therefore, biomarkers play an important role and serve as a proxy when assessing emotion and positive well-being in animals (e.g., von Borell et al, 2007 ; Schmied et al, 2008 , 2010 ; Reefmann et al, 2009a , b ; Coulon et al, 2013 , 2015 ; Briefer et al, 2015 ; Kowalik et al, 2017 ). Heart rate (HR) ( Csoltova et al, 2017 ), heart rate variability (HRV) ( Bergamasco et al, 2010 ; Katayama et al, 2016 ; Travain et al, 2016 ), surface temperature ( Travain et al, 2015 , 2016 ; Csoltova et al, 2017 ), oxytocin ( Odendaal and Meintjes, 2003 ; Mitsui et al, 2011 ; Rehn et al, 2014 ; Nagasawa et al, 2015 ), vasopressin ( Hydbring-Sandberg et al, 2004 ; MacLean et al, 2017a , b , 2018 ; Pirrone et al, 2019 ), cortisol ( Hennessy et al, 1998 , 2006 ; Coppola et al, 2006 ; Bergamasco et al, 2010 ; Shiverdecker et al, 2013 ), and alpha-amylase ( Contreras-Aguilar et al, 2017 ; Hong et al, 2019 ) have been implemented and showed potential usefulness in indirect and noninvasive assessment of positive-emotion in dogs.…”

Section: Positive-emotion Assessment In Dogsmentioning

confidence: 99%

“…As with cortisol, measuring decreased levels of alpha-amylase, a crucial salivary enzyme ( Nater and Rohleder, 2009 ), is another noninvasive approach to test sympathetic nervous system deactivation. Salivary alpha-amylase increase in response to both acute and chronic stressors has been documented in different animal species ( Fuentes et al, 2011 ; Behringer et al, 2012 ; Fuentes-Rubio et al, 2016 ; Contreras-Aguilar et al, 2018 ), including dogs ( Contreras-Aguilar et al, 2017 ; Hong et al, 2019 ). However, other studies emphasize the involvement of the parasympathetic nervous system branch in alpha-amylase secretion ( Asking and Proctor, 1989 ; Nater and Rohleder, 2009 ; Bosch et al, 2011 ).…”

Section: Positive-emotion Assessment In Dogsmentioning

confidence: 99%

Where Do We Stand in the Domestic Dog (Canis familiaris) Positive-Emotion Assessment: A State-of-the-Art Review and Future Directions

Csoltova¹,

Mehinagic²

2020

Front. Psychol.

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Although there have been a growing number of studies focusing on dog welfare, the research field concerning dog positive-emotion assessment remains mostly unexplored. This paper aims to provide a state-of-the-art review and summary of the scattered and disperse research on dog positive-emotion assessment. The review notably details the current advancement in dog positive-emotion research, what approaches, measures, methods, and techniques have been implemented so far in emotion perception, processing, and response assessment. Moreover, we propose possible future research directions for short-term emotion as well as longer-term emotional states assessment in dogs. The review ends by identifying and addressing some methodological limitations and by pointing out further methodological research needs.

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Salivary alpha-amylase as a stress biomarker in diseased dogs

Cited by 12 publications

References 11 publications

Salivary Biomarkers and Work-Related Stress in Night Shift Workers

Salivary Biomarkers and Work-Related Stress in Night Shift Workers

Evaluation of salivary vasopressin as an acute stress biomarker in healthy dogs with stress due to noise and environmental challenges

Where Do We Stand in the Domestic Dog (Canis familiaris) Positive-Emotion Assessment: A State-of-the-Art Review and Future Directions

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