Salivary and serum cystatin SA levels in patients with type 2 diabetes mellitus or diabetic nephropathy

Techatanawat, Suteera; Surarit, Rudee; Chairatvit, Kongthawat; Roytrakul, Sittiruk; Khovidhunkit, Weerapan; Thanakun, Supanee; Izumi, Yuichi; Khovidhunkit, Siribang-on Piboonniyom

doi:10.1016/j.archoralbio.2019.05.020

Cited by 8 publications

(6 citation statements)

References 39 publications

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“…This presents an additional challenge when identifying the exact position of the MA groups in the sequence. For example, the LC-MS/MS analysis identified the peptide GAAGLPGPKGERGDAGPK with a modified lysine (after trypsin digestion) but this peptide was identified at different positions in the RCPhC1 sequence, namely positions [ [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] , [39] ]; [ [58] , [59] , [60] , [61] , [62] , [63] , [64] , [65] , [66] , [67] , [68] , [69] , [70] , [71] , [72] , [73] , [74] , [75] ]; [100−117]; [157−174]; [211−228]; [247−264]; [289−306]; [346−363]; [400−417]; [436−453]; [478−495]; [535−552].…”

Section: Resultsmentioning

confidence: 99%

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Proteomics as a tool to gain next level insights into photo-crosslinkable biopolymer modifications

Pien

Bray

Gheysens

et al. 2022

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Section: Resultsmentioning

confidence: 99%

“…Polyacrylamide SDS-PAGE gel analysis revealed the typical pattern for type I collagen, with bands corresponding to the MM for COL BS pointing to β (215 kDa), α1 (130 kDa), and α2 (115 kDa) chains [ 68 , 69 ]. ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Proteomics as a tool to gain next level insights into photo-crosslinkable biopolymer modifications

Pien

Bray

Gheysens

et al. 2022

Bioactive Materials

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“…Cystatin-S (CST4) is identified as a potential biomarker in Sjögren's syndrome (autoimmune disease affecting the lacrimal and salivary producing glands) (Martini et al, 2017). Salivary CST2 has been associated with the severity of periodontal disease and its serum levels may reflect renal function (Techatanawat et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

Análise de proteínas salivares de corredores de rua após evento esportivo real

Amaral

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Martins

et al. 2021

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The aim was to characterize acute exercise-induced changes in salivary proteins of street runners. Saliva samples from 12 adult male athletes were collected before and immediately after a street race. Two groups were formed based on the distance covered, 5 km (n=4) and 10 km (n=8). Samples were subjected to depletion of amylase, albumin and immunoglobulin G. Then they were concentrated, digested and analyzed by nano-UPLC-tandem nano-ESI-MSE. A total of 69 proteins were identified. Significant changes were observed in the expression of 15 proteins in the 5 km group and 13 proteins in the 10 km group. Among the proteins with altered expression, only 7 had already been described in the literature in similar models (Alpha-Amylase 1, Lactoperoxidase, Alpha Skeletal Muscle Actin, Cystatin-B, Cystatin- SN, Cystatin- SA and Androgen-regulated Protein 3B in the submaxillary gland). This study has shown that street running induces acute changes in the salivary proteome. The results obtained add to the limited data available in the literature in the search for a better understanding of the acute effects of exercise.

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“…Cystatins and histatins act as an important first-line of defense against microorganisms in the oral cavity 46 , 47 . Lower levels of cystatin-S in saliva have been reported in children with caries as well as in patients with Sjögren’s syndrome versus healthy controls, whereas salivary cystatin-SA levels associate with periodontal status 48 , 49 . In addition, salivary levels of cystatin-D affects the subjective perception of oral dryness, and different parotid levels of cystatins and histatins has previously been reported in two irradiated patients versus two healthy controls 50 , 51 .…”

Section: Discussionmentioning

confidence: 99%

Intraglandular mesenchymal stem cell treatment induces changes in the salivary proteome of irradiated patients

et al. 2022

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Background Hyposalivation and xerostomia (dry mouth), are the leading site-effects to treatment of head and neck cancer. Currently, there are no effective therapies to alleviate radiation-induced hyposalivation. Adipose tissue-derived mesenchymal stem/stromal cells (AT-MSCs) have shown potential for restoring salivary gland function. However, the mode of action is unknown. The purpose of the present study was therefore to characterize the effect of AT-MSC therapy on the salivary proteome in previously irradiated head and neck cancer patients. Methods Whole saliva was collected from patients with radiation-induced salivary gland hypofunction (n = 8) at baseline, and 120 days after AT-MSC treatment, and from healthy controls (n = 10). The salivary proteome was characterized with mass spectrometry based proteomics, and data was compared within the AT-MSC group (baseline versus day 120) and between AT-MSC group and healthy controls. Significance levels between groups were determined by using double-sided t-test, and visualized by means of principal component analysis, volcano plots and cluster analysis. Results Here we show that 140 human proteins are significantly differentially expressed in saliva from patients with radiation-induced hypofunction versus healthy controls. AT-MSC treatment induce a significant impact on the salivary proteome, as 99 proteins are differentially expressed at baseline vs. 120 days after treatment. However, AT-MSC treatment does not restore healthy conditions, as 212 proteins are significantly differentially expressed in saliva 120 days after AT-MSCs treatment, as compared to healthy controls. Conclusion The results indicate an increase in proteins related to tissue regeneration in AT-MSCs treated patients. Our study demonstrates the impact of AT-MSCs on the salivary proteome, thereby providing insight into the potential mode of action of this novel treatment approach.

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Salivary and serum cystatin SA levels in patients with type 2 diabetes mellitus or diabetic nephropathy

Cited by 8 publications

References 39 publications

Proteomics as a tool to gain next level insights into photo-crosslinkable biopolymer modifications

Proteomics as a tool to gain next level insights into photo-crosslinkable biopolymer modifications

Análise de proteínas salivares de corredores de rua após evento esportivo real

Intraglandular mesenchymal stem cell treatment induces changes in the salivary proteome of irradiated patients

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