Salivary Antioxidative Index in Newborns at Risk of Sepsis as Novel Parameter for Early-Onset Neonatal Sepsis

Yunanto, Ari; Firdaus, Rizky Taufan; Triawanti, Triawanti; Suhartono, Eko

doi:10.12720/jomb.3.1.63-66

Cited by 3 publications

(4 citation statements)

References 14 publications

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“…Salivary antioxidative index is ratio of salivary enzymatic antioxidant activity to salivary hydrogen peroxide level, shows that any decrease of SAOI will contribute presence of sepsis about 3,7 fold. This study suggests a marker role of SAOI to detect oxidative stress on early-onset of neonatal sepsis [21].…”

Section: Discussionmentioning

confidence: 67%

Advance Oxidation Protein Products (AOPPs) of Newborn at Risk of Sepsis as Novel Parameter for Early-Onset Neonatal Sepsis

Yunanto¹

2014

IJBBB

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Abstract-Neonatal sepsis is a clinical syndrome of bacteremia and divided into two different types, early-onset neonatal sepsis and late-onset neonatal sepsis. Sepsis promotes the unbalanced production of oxidant and anti-oxidant substances, causing an excess of free oxygen compounds. Early markers of neonatal sepsis have been studied in recent years, and this study proposed another parameter to detect early-onset neonatal sepsis with the use of advance oxidation protein products (AOPPs). This study was conducted in April -June 2012, saliva and serum specimens were taken from 81 newborns, in which 39 infants were at risk of sepsis and 42 infants were healthy and served as a control group. Data was analyzed by using Mann-Whitney test and Spearman correlation test. This study suggested that there is significant difference between AOPPs level on the case group compare to the control group. This study also found that there is significant difference between salivary AOPPs level compare to serum AOPPs level. As the conclusion, this study proposed that the AOPPs parameter may be used as another marker to detect early-onset neonatal sepsis.Index Terms-Early-onset neonatal sepsis, free oxygen compounds, AOPPs.

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Section: Discussionmentioning

confidence: 67%

Advance Oxidation Protein Products (AOPPs) of Newborn at Risk of Sepsis as Novel Parameter for Early-Onset Neonatal Sepsis

Yunanto¹

2014

IJBBB

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“…9 The production of ROS in neonatal sepsis has been documented in various studies, including our previous studies that have shown an increase in ROS such as peroxide in neonatal sepsis. 10 The increased ROS level is one of the predisposing factors for disease severity. 11 An excessive increase in ROS will activate endogenous enzymatic antioxidants such as Catalase (CAT) to prevent the accumulation of ROS in the body.…”

Section: Introductionmentioning

confidence: 99%

The effect of antibiotic therapy on Salivary Catalase kinetic parameters in neonatal at risk of Sepsis

Yunanto¹,

Halim²,

Iskandar³

et al. 2020

InaJPerinatol

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Introduction: This study describes the effect of antibiotic therapy on salivary catalase kinetic parameters (CAT) at neonatal at risk of sepsis. Methods: Study is conducted from February – June 2015. Salivary samples are obtained from 20 neonates (5 normal-healthy neonates and 15 neonates at risk of sepsis) at Ulin General Hospital, Banjarmasin, Indonesia. The samples were placed in four different groups: P0 as control group (saliva + hydrogen peroxide/H2O2), P1 (saliva + 2 mg Ampicillin + H2O2), P2 (saliva + 0.2 mg Gentamicin + H2O2), P3 (saliva + 2 mg Ampicillin + 0.2 mg Gentamicin + H2O2). The solution is incubated at 37oC and 40oC before catalase (CAT) activity is measured. Catalase activity is measured by using a spectrophotometer at 240 nm. Kinetic parameters are measured at different concentrations of H2O2 and temperature. Kinetic parameters are represented by the Michaelis-Menten constant (Km) and the maximum reaction speed (Vmax) obtained through the Lineweaver-Burk curve plot. Results: The Km of Catalase on the saliva of neonates at risk of sepsis treated with antibiotics (4.37, 1.84, 0.12, and 0.23, 3.74, 1.5, for P1, P2, P3 respectively) was lower than the control group (17.61 and 12.54), both at 37ºC and 40ºC. Similarly, Vmax of the neonates at risk of sepsis treated with antibiotics (0.46, 0.34, 0.04 and 0.07, 0.20, 0.24) was lower than the control group (1.47 and 0.53) at 37ºC and 40ºC. Conclusion: The study shows that the Catalase activity at the saliva of newborns at risk of sepsis treated with antibiotic was lower than the control group.

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“…8,9 NADPH oxidase is involved in the formation of several ROS such as hydrogen peroxide (H2O2), while MPO was involved in the formation of another ROS such as HOCl. 10 HOCl leads to chlorinative stress. Both oxidative and chlorinative stress leads to lipid peroxidation.…”

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confidence: 99%

“…Both oxidative and chlorinative stress leads to lipid peroxidation. 10 This process was characterised by the formation of some compounds such as malondialdehyde (MDA). 11 MDA is an indicator for ROS-induced lipid peroxidation.…”

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Significance of Plasma Antioxidants in Pathogenesis of Dengue Haemorrhagic Fever

Singh¹,

Singh²

2018

jemds

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BACKGROUND Dengue is a major cause of morbidity in tropical and subtropical regions. It has been shown that there is a relationship between immune and oxidative mechanisms leading to altered immune responses. The role of free radicals in the pathogenesis of dengue fever in children is lacking. Aim-This study was done with the objective of determining various oxidants and antioxidants and evaluating their role in pathogenesis of severe form of Dengue Fever, Dengue Haemorrhagic Fever. MATERIALS AND METHODS 30 patients with Dengue Haemorrhagic Fever (DHF) in the age group of 0-16 years, having clinical features of DHF and positive findings in blood test were included in this study. Another 30 age and gender-matched children were included in this study to serve as controls. Levels of plasma MDA, protein carbonyl, nitrite, H2O2 and MPO activity were estimated by different standard methods. Statistical analysis was done with the help of t-test. Settings-This study was done in Mayo Institute of Medical Sciences, Lucknow. Design-A prospective, institution-based study. RESULTS The mean age of presentation of children with DHF was comparable to control values. The mean plasma levels of MDA, protein carbonyl, nitrite, Cu, H2O2 and MPO activity were significantly higher in patients with DHF as compared to control values (p < 0.05 each). CONCLUSION It can be concluded that imbalances between prooxidants and antioxidants play a significant role in pathogenesis of children with DHF.

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Salivary Antioxidative Index in Newborns at Risk of Sepsis as Novel Parameter for Early-Onset Neonatal Sepsis

Cited by 3 publications

References 14 publications

Advance Oxidation Protein Products (AOPPs) of Newborn at Risk of Sepsis as Novel Parameter for Early-Onset Neonatal Sepsis

Advance Oxidation Protein Products (AOPPs) of Newborn at Risk of Sepsis as Novel Parameter for Early-Onset Neonatal Sepsis

The effect of antibiotic therapy on Salivary Catalase kinetic parameters in neonatal at risk of Sepsis

Significance of Plasma Antioxidants in Pathogenesis of Dengue Haemorrhagic Fever

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