Salivary Auto-Antibodies as Noninvasive Diagnostic Markers of Oral Cavity Squamous Cell Carcinoma

Abstract

Background: Oral cavity squamous cell carcinoma (OSCC) is one of the most common cancers worldwide, and its incidence is still increasing. Approximately 50% of patients with OSCC die within 5 years after diagnosis, mostly ascribed to the fact that the majority of patients present advanced stages of OSCC at the time of diagnosis.Methods: To discover salivary biomarkers for ameliorating the detection of OSCC, herein, we developed a multiplexed bead-based platform to simultaneously detect auto-antibodies (auto-Abs) in salivary samples.Results: Compared with healthy individuals, the salivary levels of anti-p53, anti-survivin, anti-Hsp60, and anti-RPLP0 were significantly elevated in patients with OSCC. Noteworthily, the elevated levels of anti-p53, anti-survivin, and anti-Hsp60 were already observed in individuals with oral potentially malignant disorder. Moreover, the salivary levels of anti-p53, anti-survivin, anti-Hsp60, anti-RPLP0, and anti-CK8 were significantly elevated in patients with early-stage OSCC compared with those in healthy individuals. Most importantly, the use of a combined panel of salivary anti-p53, anti-survivin, anti-Hsp60, and anti-RPLP0 largely improves the detection of OSCC.Conclusion: Collectively, our results reveal that the salivary auto-Abs are effective OSCC biomarkers and the four-auto-Ab panel provides a novel and practicable approach for OSCC screening.Impact: This study provides the first evidence for the potential clinical application of salivary auto-Abs in OSCC diagnosis. Cancer Epidemiol Biomarkers Prev; 23(8); 1569-78. Ó2014 AACR.

Proteomic and N‐glycoproteomic quantification reveal aberrant changes in the human saliva of oral ulcer patients

“…It has been reported that oral ulcers always occur in association with many diseases and could be caused by different mechanisms including local trauma and aphthous stomatitis. The alteration of the global proteome in human saliva in different diseases were demonstrated by previous studies . In this work, we explored the proteome and N‐glycoproteome of human saliva and tried to reveal the molecular effect of oral ulcer in protein and glycoprotein level.…”

Survivin, a molecular target for therapeutic interventions in squamous cell carcinoma

“…For example, sensitive diagnostic tests have been developed that are based on the presence of survivin in urine of bladder cancer patients [116, 117]. Similarly, presence of survivin auto-antibodies in the saliva of OSCC patients provides a novel and practicable approach for OSCC screening [118, 119]. In addition, anti-survivin antibodies were also detected in the circulating blood of cancer patients [120], which can be exploited as a cancer diagnostic tool.…”

Saliva proteome profiling reveals potential salivary biomarkers for detection of oral cavity squamous cell carcinoma

“…Although accessing the biomarkers in body fluids could be a practical approach for cancer detection , no candidate marker of OSCC has yet been approved for clinical use. In spite of many efforts made for discovery of OSCC biomarkers, unfortunately, most of the to‐date‐identified biomarkers are not effective enough to detect OSCC at early stage . In this regard, identification of useful biomarkers for early detection of OSCC is still in urgent need.…”