Salivary biomarkers of existing periodontal disease

Miller, Craig S.; King, Charles P.; Langub, M. Chris; Kryscio, Richard J.; Thomas, Mark V.

doi:10.14219/jada.archive.2006.0181

Cited by 333 publications

(391 citation statements)

References 60 publications

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“…Saliva is composed mainly of water, electrolytes, glycoprotein, and antimicrobial enzymes 4,5 . Several studies have been shown that there is a relationship between quantitative changes of salivary components and some oral disease 6,7 . Saliva can be used as a noninvasive diagnostic fluid to measure chemical biomarkers released during periodontal disease 6,8 .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of salivary α-L-Fucose and its related parameters in periodontitis

Wsoo

Ahmed

2013

ZJMS

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Section: Introductionmentioning

confidence: 99%

Evaluation of salivary α-L-Fucose and its related parameters in periodontitis

Wsoo

Ahmed

2013

ZJMS

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“…A coleta da STE é mais rápida e fácil, porém os estímulos utilizados podem alterar o pH ou exacerbar a secreção de água, diluindo os análitos de interesse (Miller, King et al 2006). A relação entre as concentrações de alguns marcadores inflamatórios encontradas na saliva total não estimulada e na STE, assim como a relação entre a saliva total não estimulada e o soro, pode ser visualizada na figura 2.…”

Section: Saliva E Biomarcadoresunclassified

“…Resultados obtidos de amostras de fluidos de 45 adultos saudáveis [reproduzido de (Miller, King et al 2006)]. …”

Section: Saliva E Biomarcadoresunclassified

“…Artigos de revisão já trazem idéias ou aparelhos capazes de fazer medições rápidas e seguras (Miller, King et al 2006;Segal and Wong 2008). Por exemplo, existe um equipamento descrito por Herr et al (Herr, Hatch et al 2007) que integra um pré-tratamento de amostras de saliva (filtração, homogenização e enriquecimento) com um ensaio de imuno-eletroforese.…”

Section: Saliva E Biomarcadoresunclassified

“…Alguns trabalhos relatam que os níveis de MMP-8 na saliva estariam altamente correlacionados com os parâmetros clínicos encontrados na DP, sendo que elevados níveis de MMP-8 aumentariam o risco de DP (Miller, King et al 2006).…”

Section: Steunclassified

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Concentrações de MMP-8, MMP-9, MPO, TIMP-1 e TIMP-2 na saliva e correlações com plasma

Meschiari¹

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Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases known to cleave components of the connective tissue in physiological and pathological processes. The regulation of MMP activities is done by the tissue inhibitors of metalloproteinases (TIMPs).Periodontal disease (PD) is a chronic inflammation with excessive activity of MMPs, which degrade the tooth supporting tissues.The saliva components are derived from the local blood supply, and this fluid may therefore reflect the plasma. So, the objectives of this study were: 1) to compare the levels of MMPs, TIMPs and MPO in stimulated whole saliva (SWS) and plasma of PD patients before (PB) and 3 months after (PT) the non-surgical periodontal treatment, and in healthy volunteers at baseline (CB) and 3 months after baseline (CT), and 2) to evaluate the correlations between the results found.Measurements of MMP-8, MMP-9, TIMP-1 and TIMP-2 were performed by ELISA. Gelatinolitic activity of MMP-9 forms were determined by zymography, and the MPO activity was determined by colorimetric assay.There was lower gelatinolitic activity, and lower concentrations of MMP-8 and TIMP-2 in STE on PT group compared with PB group (p <0.05). MPO activity was higher in PB compared to CB (p <0.05).Statistically significant moderate correlations were observed in all associations between MMP-9 performed by ELISA in plasma and gelatinolitic activity bands of STE: MMP-9 molecular form of 92 kDa (r = 0,37, p = 0,0017), MMP-9 molecular form of 130 kDa (r = 0,35, p = 0,003), the sum of its gelatinase activity (130 +92 kDa) (r = 0,43, p = 0,0002), gelatinase of 180 kDa (r = 0,35, p = 0,003), and the sum of total gelatinase activity (180+130+92 kDa) (r = 0,37, p = 0,002). Circulating MMP-8 levels correlate with salivary gelatinase activity bands of 92 kDa (r = 0,30, p = 0,01) and the sum of gelatinase activity (130 +92 kDa) (r = 0,24, p = 0,04). In addition, a weak correlation between gelatinase activity of plasmatic 92 kDa MMP-9 and gelatinase of 180 kDa in SWS (r= 0,26 p = 0,03) was found, and a moderate correlation between plasmatic TIMP-2 and TIMP-2 of SWS (r = 0,32, p = 0,004).The results show that the gelatinase activity of SWS may reflect the concentrations of systemic inflammatory markers such as MMP-8 and MMP-9, also the concentration of TIMP-2 in the SWS may reflect the circulating concentration of TIMP-2. The evaluation of these results suggests that there is an attenuation of some inflammatory markers analyzed in the SWS and in plasma after treatment of PD. Furthermore, there are correlations between salivary and plasma levels in some of these markers. Because saliva sampling is less invasive, more studies about the correlations between these markers in these two fluids are necessary. Key words: saliva, plasma, matrix metalloproteinases, periodontal disease. LISTA DE ABREVIATURAS, SIGLAS E SÍMBOLOS BSA -

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Diagnosis of Periodontal Diseases

Lai¹

2012

Comprehensive Preventive Dentistry

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Salivary biomarkers of existing periodontal disease

Cited by 333 publications

References 60 publications

Evaluation of salivary α-L-Fucose and its related parameters in periodontitis

Evaluation of salivary α-L-Fucose and its related parameters in periodontitis

Concentrações de MMP-8, MMP-9, MPO, TIMP-1 e TIMP-2 na saliva e correlações com plasma

Diagnosis of Periodontal Diseases

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