Salivary Biomarkers to Assess Breast Cancer Diagnosis and Progression: Are We There Yet?

Streckfus, Charles F.

doi:10.5772/intechopen.85762

Cited by 5 publications

(3 citation statements)

References 52 publications

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“…Most studies on the analysis of saliva in breast cancer were aimed at identifying biomarkers that can differentiate patients with breast cancer from healthy controls [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ]. Previously, we identified 11 metabolites that allow us to do this with a sensitivity of up to 91% [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…The literature provides data on the study of the composition of saliva in breast cancer [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ]. Saliva biomarkers have been shown to achieve a sensitivity of 73% (72–74) and a specificity of 74% (72–76) in the diagnosis of breast cancer [ 45 ].…”

Section: Introductionmentioning

confidence: 99%

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«Salivaomics» of Different Molecular Biological Subtypes of Breast Cancer

Bel’skaya

Sarf

2022

CIMB

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The aim of the study was to determine the metabolic characteristics of saliva depending on the molecular biological subtype of breast cancer, as well as depending on the expression levels of HER2, estrogen receptors (ER), and progesterone receptors (PR). The study included 487 patients with morphologically verified breast cancer and 298 volunteers without breast pathologies. Saliva samples were obtained from all patients strictly before the start of treatment and the values of 42 biochemical indicators were determined. It has been established that the saliva of healthy volunteers and patients with various molecular biological subtypes of breast cancer differs in 12 biochemical indicators: concentrations of protein, urea, nitric oxide, malondialdehyde, total amino acid content, and activity of lactate dehydrogenase, alkaline phosphatase, gamma-glutamyltransferase, catalase, amylase, superoxide dismutase, and peroxidases. The saliva composition of patients with basal-like breast cancer differs from other subtypes in terms of the maximum number of indicators. Changes in biochemical indicators indicated an increase in the processes of lipid peroxidation and endogenous intoxication and a weakening of antioxidant protection, which correlates with the severity of the disease and the least favorable prognosis for this subtype of breast cancer. An analysis was made of the individual contribution of the expression level of HER2, estrogen, and progesterone receptors to changes in the biochemical composition of saliva. The HER2 (−)/HER2 (+) group, which should be considered as a single group, as well as ER-positive breast cancer, differ statistically significantly from the control group. For ER/PR-positive breast cancer, a more favorable ratio of saliva biochemical indicators was also noted compared to ER/PR-negative breast cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

«Salivaomics» of Different Molecular Biological Subtypes of Breast Cancer

Bel’skaya

Sarf

2022

CIMB

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“…A study carried out in 2000 showed that a salivary proteomics-based panel of biomarkers, consisting of ERBB2/HER2, cancer antigen 15-3 (CA15-3), and tumor suppressor oncogene protein 53 ( TP53 /p53), has a potential use in the initial detection and follow-up screening for the detection of BC in women based on ELISA detection [ 222 ]. Today, isotopic labeling coupled with liquid chromatography tandem mass spectrometry (IL-LC-MS/MS) is often used to characterize the salivary protein profiles in BC patients [ 223 ]. Salivary protein biomarkers are known to be useful for discriminating between healthy controls, fibroadenomas and incipient ductal carcinoma lesions [ 224 ], and lymph-node-positive and lymph-node-negative patients with ductal carcinoma [ 225 ], as well as to assess the differences between the salivary protein profiles of HER2/ neu -receptor-positive and HER2/neu-receptor-negative patients [ 226 ].…”

Section: Breast Cancer Investigation In the Multi-omics Eramentioning

confidence: 99%

Omics-Based Investigations of Breast Cancer

Neagu,

Whitham,

Bruno

et al. 2023

Molecules

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Breast cancer (BC) is characterized by an extensive genotypic and phenotypic heterogeneity. In-depth investigations into the molecular bases of BC phenotypes, carcinogenesis, progression, and metastasis are necessary for accurate diagnoses, prognoses, and therapy assessments in predictive, precision, and personalized oncology. This review discusses both classic as well as several novel omics fields that are involved or should be used in modern BC investigations, which may be integrated as a holistic term, onco-breastomics. Rapid and recent advances in molecular profiling strategies and analytical techniques based on high-throughput sequencing and mass spectrometry (MS) development have generated large-scale multi-omics datasets, mainly emerging from the three ”big omics”, based on the central dogma of molecular biology: genomics, transcriptomics, and proteomics. Metabolomics-based approaches also reflect the dynamic response of BC cells to genetic modifications. Interactomics promotes a holistic view in BC research by constructing and characterizing protein–protein interaction (PPI) networks that provide a novel hypothesis for the pathophysiological processes involved in BC progression and subtyping. The emergence of new omics- and epiomics-based multidimensional approaches provide opportunities to gain insights into BC heterogeneity and its underlying mechanisms. The three main epiomics fields (epigenomics, epitranscriptomics, and epiproteomics) are focused on the epigenetic DNA changes, RNAs modifications, and posttranslational modifications (PTMs) affecting protein functions for an in-depth understanding of cancer cell proliferation, migration, and invasion. Novel omics fields, such as epichaperomics or epimetabolomics, could investigate the modifications in the interactome induced by stressors and provide PPI changes, as well as in metabolites, as drivers of BC-causing phenotypes. Over the last years, several proteomics-derived omics, such as matrisomics, exosomics, secretomics, kinomics, phosphoproteomics, or immunomics, provided valuable data for a deep understanding of dysregulated pathways in BC cells and their tumor microenvironment (TME) or tumor immune microenvironment (TIMW). Most of these omics datasets are still assessed individually using distinct approches and do not generate the desired and expected global-integrative knowledge with applications in clinical diagnostics. However, several hyphenated omics approaches, such as proteo-genomics, proteo-transcriptomics, and phosphoproteomics-exosomics are useful for the identification of putative BC biomarkers and therapeutic targets. To develop non-invasive diagnostic tests and to discover new biomarkers for BC, classic and novel omics-based strategies allow for significant advances in blood/plasma-based omics. Salivaomics, urinomics, and milkomics appear as integrative omics that may develop a high potential for early and non-invasive diagnoses in BC. Thus, the analysis of the tumor circulome is considered a novel frontier in liquid biopsy. Omics-based investigations have applications in BC modeling, as well as accurate BC classification and subtype characterization. The future in omics-based investigations of BC may be also focused on multi-omics single-cell analyses.

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Salivary exosomes: properties, medical applications, and isolation methods

Cheshmi¹,

Cheshomi²

2020

Mol Biol Rep

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Salivary Biomarkers to Assess Breast Cancer Diagnosis and Progression: Are We There Yet?

Cited by 5 publications

References 52 publications

«Salivaomics» of Different Molecular Biological Subtypes of Breast Cancer

«Salivaomics» of Different Molecular Biological Subtypes of Breast Cancer

Omics-Based Investigations of Breast Cancer

Salivary exosomes: properties, medical applications, and isolation methods

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