Salivary Bioscience and Periodontal Medicine

Wei, Chen Xuan; Yu, Yonghao; Aleti, Gajender; Torralba, Manny; Edlund, Anna; Nelson, Karen E.; Freire, Marcelo

doi:10.1007/978-3-030-35784-9_18

Cited by 3 publications

(3 citation statements)

References 169 publications

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“…Mammalian saliva carries a precise developmental and biological program that defines the function of multiple organs. Saliva is at the interface of dynamic interactions between microbial ecosystems and oral immune cells [1]. Dysregulation of saliva physiology has an impact on oral and systemic health, which makes it a potential tool for the diagnosis of oral and systemic diseases [65].…”

Section: Challenges and Future Perspectivesmentioning

confidence: 99%

“…The oral cavity serves as a window to the human body where observations made upon analysis of oral fluids could potentially reflect the health, disease or stressful psychological status of the person being investigated. For instance, saliva carries precise developmental and biological information that defines the function of multiple organs including oral and periodontal tissues [1]. Salivary pellicle, also known as acquired enamel pellicle, is an important interface structure/proteinaceous material located between dental hard tissue surfaces and the oral environment which modulates the biological and microbiological activities on tooth surfaces [2].…”

Section: Introductionmentioning

confidence: 99%

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Analysing Complex Oral Protein Samples: Complete Workflow and Case Analysis of Salivary Pellicles

Wei

Burrow

Botelho

et al. 2021

JCM

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Studies on small quantity, highly complex protein samples, such as salivary pellicle, have been enabled by recent major technological and analytical breakthroughs. Advances in mass spectrometry-based computational proteomics such as Multidimensional Protein Identification Technology have allowed precise identification and quantification of complex protein samples on a proteome-wide scale, which has enabled the determination of corresponding genes and cellular functions at the protein level. The latter was achieved via protein-protein interaction mapping with Gene Ontology annotation. In recent years, the application of these technologies has broken various barriers in small-quantity-complex-protein research such as salivary pellicle. This review provides a concise summary of contemporary proteomic techniques contributing to (1) increased complex protein (up to hundreds) identification using minute sample sizes (µg level), (2) precise protein quantification by advanced stable isotope labelling or label-free approaches and (3) the emerging concepts and techniques regarding computational integration, such as the Gene Ontology Consortium and protein-protein interaction mapping. The latter integrates the structural, genomic, and biological context of proteins and genes to predict protein interactions and functional connections in a given biological context. The same technological breakthroughs and computational integration concepts can also be applied to other low-volume oral protein complexes such as gingival crevicular or peri-implant sulcular fluids.

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Section: Challenges and Future Perspectivesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysing Complex Oral Protein Samples: Complete Workflow and Case Analysis of Salivary Pellicles

Wei

Burrow

Botelho

et al. 2021

JCM

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show abstract

“…We characterized global immune and proteome responses after SARS-CoV-2 infection in matched plasma and saliva obtained from convalescent COVID-19 subjects (saliva, n = 42; plasma, n = 42), with samples obtained from healthy individuals pre-COVID-19 era serving as healthy controls (saliva, n = 13; plasma = 13). We focused on the analysis of saliva in addition to plasma for the following reasons: 1) saliva is a practical and optimal body fluid to monitor for host and immune-inflammatory markers 2) salivary fluid is a direct surrogate for SARS-CoV-2 antibody responses derived from bronchial-alveolar lymphoid tissues (BALT) [ 7 ], 3) oral fluid can reflect systemic reactions to infection since more than 90% of body protein components are detected from saliva [ [8] , [9] , [10] ], 4) saliva contains oral microbiome commensals which shape the host immune profile [ 11 , 12 ], and 5) oral inflammation can influence the severity of systemic inflammatory responses [ [13] , [14] , [15] ]. A human salivary proteome database was initially developed to explore saliva as a source of mapping markers in health and disease and to further advance comparisons to other body fluids [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Persistent immune and clotting dysfunction detected in saliva and blood plasma after COVID-19

Jang¹,

Choudhury²,

Yoshida³

et al. 2023

Heliyon

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Persistent Immune and Clotting Dysfunction Detected in Saliva and Blood Plasma after COVID-19

Jang

Choudhury

et al. 2022

Preprint

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A growing number of studies indicate that coronavirus disease 2019 (COVID-19) is associated with inflammatory sequelae, but molecular signatures governing the normal vs. pathologic convalescence process have not been well-delineated. We characterized global immune and proteome responses in matched plasma and saliva samples obtained from COVID-19 patients collected between 4-6 weeks after initial clinical symptoms resolved. Convalescent subjects showed robust IgA and IgG responses and positive antibody correlations between matched saliva and plasma samples. However, global shotgun proteomics revealed persistent inflammatory patterns in convalescent samples including dysfunction of salivary innate immune cells and clotting factors in plasma (e.g., fibrinogen and antithrombin), with positive correlations to acute COVID-19 disease severity. Saliva samples were characterized by higher concentrations of IgA, and proteomics showed altered pathways that correlated positively with IgA levels. Our study positions saliva as a viable fluid to monitor immunity beyond plasma to document COVID-19 immune, inflammatory, and coagulation-related sequelae.

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Salivary Bioscience and Periodontal Medicine

Cited by 3 publications

References 169 publications

Analysing Complex Oral Protein Samples: Complete Workflow and Case Analysis of Salivary Pellicles

Analysing Complex Oral Protein Samples: Complete Workflow and Case Analysis of Salivary Pellicles

Persistent immune and clotting dysfunction detected in saliva and blood plasma after COVID-19

Persistent Immune and Clotting Dysfunction Detected in Saliva and Blood Plasma after COVID-19

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