Salivary digestive enzymes of the wheat bug, Eurygaster integriceps (Insecta: Hemiptera: Scutelleridae)

Mehrabadi, Mohammad; Bandani, Ali Reza; Dastranj, Mehdi

doi:10.1016/j.crvi.2014.04.003

Cited by 16 publications

(17 citation statements)

References 38 publications

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“…Daily intake was significantly lower at the 5 th instar than the 4 th , and then increased slightly at adulthood. Mehrabadi et al found that activities of salivary enzymes were increased in response to feeding in comparison to starved insects, and held their production was induced by the feeding pulse [39]. Our study showed that pectinase, amylase, cellulase and protease activities in saliva were highest in 3 rd instar nymphs, and cellulase and proteinase had a certain relationship with daily food consumption.…”

Section: Discussionsupporting

confidence: 55%

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Levels of Salivary Enzymes of Apolygus Lucorum (Hemiptera: Miridae), From 1st Instar Nymph to Adult, and Their Potential Relation to Bug Feeding

Tan¹,

Xu²,

Gao³

et al. 2016

PLoS ONE

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In recent years, Apolygus lucorum has caused increasing damage to cotton and fruit trees in China. The salivary enzymes secreted by A. lucorum when sucking on host plants induce a series of biochemical reactions in plants, and the pre-oral digestion benefits the bug feeding. In this study, the food intake of A. lucorum from 1st instar nymphs to adults was measured, and the corresponding salivary activity of pectinase, amylase, cellulase, protease, polyphenol oxidase and peroxidase was determined. Daily food intake varied with developmental stage, peaking in 3rd and 4th instar nymphs. Pectinase, amylase, cellulase and protease were detected in both nymphal and adult saliva of A. lucorum, while neither polyphenol oxidase nor peroxidase was detected. Protease activity varied with food intake peaking at the 3rd-4th instar, and then slightly decreasing at the 5th instar. Levels of pectinase, amylase and cellulase increased significantly with the daily feeding level until the 3rd instar, corresponding with increasing damage to host plants. The activity of both cellulase and protease had a significant linear relationship with the average daily food intake. The increasing activity of enzymes in saliva explain stage-specific impacts of A. lucorum on the host plants, and suggest that optimal management of A. lucorum would be confined to its control threshold prior to the peak of daily feeding in the 3rd instar.

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Section: Discussionsupporting

confidence: 55%

“…Physiological structure and characterization of salivary gland complexes (SGC) of Eurygaster integriceps with its piercing–sucking mode of feeding showed advantages of this feeding strategy in pre-oral digestion, and the activities of salivary enzymes were increased in response to feeding [39]. Boyd etc.…”

Section: Discussionmentioning

confidence: 99%

Levels of Salivary Enzymes of Apolygus Lucorum (Hemiptera: Miridae), From 1st Instar Nymph to Adult, and Their Potential Relation to Bug Feeding

Tan¹,

Xu²,

Gao³

et al. 2016

PLoS ONE

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“…The digestion-related common DEGs encoded five lipases, three phospholipases, two carbohydrases, one maltase, one galactosidase, one mannosidase, and three serine proteases. It Tmakes sense since many of them (e.g., maltase, galactosidase, mannosidase, lipase and phospholipase) are essential digestive enzymes for glycolysis or esterlysis, and critical to the development and reproduction of insects [44][45][46][47][48][49]. Differential expression of a significant number of digestion-related genes was also identified among different biotypes in the whitefly Bemisia tabaci and fruit fly Drosophila patchea [50,51].…”

Section: Divergence Of Aphid Biotypesmentioning

confidence: 99%

Transcriptome Profiling Revealed Potentially Critical Roles for Digestion and Defense-Related Genes in Insects’ Use of Resistant Host Plants: A Case Study with Sitobion Avenae

Wang

Shi

Liu

et al. 2020

Insects

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Using host plant resistance (HPR) in management of insect pests is often environmentally friendly and suitable for sustainable development of agricultural industries. However, this strategy can be limited by rapid evolution of insect populations that overcome HPR, for which the underlying molecular factors and mechanisms are not well understood. To address this issue, we analyzed transcriptomes of two distinct biotypes of the grain aphid, Sitobion avenae (Fabricius), on wheat and barley. This analysis revealed a large number of differentially expressed genes (DEGs) between biotypes 1 and 3 on wheat and barley. The majority of them were common DEGs occurring on both wheat and barley. GO and KEGG enrichment analyses for these common DEGs demonstrated significant expression divergence between both biotypes in genes associated with digestion and defense. Top defense-related common DEGs with the most significant expression changes included three peroxidases, two UGTs (UDP-glycosyltransferase), two cuticle proteins, one glutathione S-transferases (GST), one superoxide dismutase, and one esterase, suggesting their potentially critical roles in the divergence of S. avenae biotypes. A relatively high number of specific DEGs on wheat were identified for peroxidases (9) and P450s (8), indicating that phenolic compounds and hydroxamic acids may play key roles in resistance of wheat against S. avenae. Enrichment of specific DEGs on barley for P450s and ABC transporters suggested their key roles in this aphid’s detoxification against secondary metabolites (e.g., alkaloids) in barley. Our results can provide insights into the molecular factors and functions that explain biotype adaptation in insects and their use of resistant plants. This study also has significant implications for developing new resistant cultivars, developing strategies that limit rapid development of insect biotypes, and extending resistant crop cultivars’ durability and sustainability in integrated management programs.

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“…У клопов амилазы синтезируются в двух независимых отделах пищеварительного тракта -слюнных железах и кишечнике (Вилкова, 1980;Ravan et al, 2009;Ramzi et al, 2016;Li et al, 2017). α-Амилазы, как и другие гидролазы хлебных клопов, изучаются довольно давно и многими авторами (Вилкова 1980;Конарев, 1981;Konarev, 1996;Mehrabadi et al, 2014), однако данные по ним более скудны и разрознены по сравнению со такими ферментами у кровососущих, хищных и других растительноядных клопов. Еще не отсеквенированы геномы представителей родов Eurygaster и Aelia Fabricius, что существенно затрудняет анализ их ферментативных систем.…”

Section: α-амилазыunclassified

“…Помимо протеаз слюнные железы вредной черепашки вырабатывают α-амилазы и липазы (Вилкова, 1968, 1979Павлюшин и др. 2015;Конарев и Фомичева, 1991;Mehrabadi et al, 2014). К сожалению, протеом и транскриптом вредной черепашки еще недостаточно изучены, однако известно, что в слюнных железах ряда видов растительноядных Hemiptera синтезируются и другие пищеварительные гидролазы, в т.ч.…”

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Digestive Hydrolases of Wheat Bugs: Properties, Significance and Possible Ways to Limit Their Activity

Konarev¹

2020

PPN

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Salivary digestive enzymes of the wheat bug, Eurygaster integriceps (Insecta: Hemiptera: Scutelleridae)

Cited by 16 publications

References 38 publications

Levels of Salivary Enzymes of Apolygus Lucorum (Hemiptera: Miridae), From 1st Instar Nymph to Adult, and Their Potential Relation to Bug Feeding

Levels of Salivary Enzymes of Apolygus Lucorum (Hemiptera: Miridae), From 1st Instar Nymph to Adult, and Their Potential Relation to Bug Feeding

Transcriptome Profiling Revealed Potentially Critical Roles for Digestion and Defense-Related Genes in Insects’ Use of Resistant Host Plants: A Case Study with Sitobion Avenae

Digestive Hydrolases of Wheat Bugs: Properties, Significance and Possible Ways to Limit Their Activity

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