The diamondback moth (Plutella xylostella) and small white cabbage butterfly (Pieris rapae) are the two main serious pests of cruciferous crops (Brassicaceae) that have developed resistance to chemical control methods. In order to avoid such resistance and also the adverse effects of chemical pesticides on the environment, alternative methods have usually been suggested, including the use of plant enzyme inhibitors. Here, the inhibitory effects of proteinaceous inhibitors extracted from wheat, canola, sesame, bean and triticale were evaluated against the digestive α-amylases, larval growth, development and nutritional indecs of the diamondback moth and small white cabbage butterfly. Our results indicated that triticale and wheat extracts inhibited α-amylolytic activity in an alkaline pH, which is in accordance with the moth and butterfly gut α-amylase optimum pH. Dose-dependent inhibition of two crucifer pests by triticale and wheat was observed using spectrophotometry and gel electrophoresis. Implementation of specificity studies showed that wheat and triticale-proteinaceous extract were inactive against Chinese and purple cabbage amylase. Triticale and wheat were resistant against insects' gut proteases. Results of the feeding bioassay indicated that triticale-proteinaceous extract could cause a significant reduction in survival and larval body mass. The results of the nutritional indecs also showed larvae of both species that fed on a Triticale proteinaceous inhibitor-treated diet had the lowest values for the efficiency of conversion of ingested food and relative growth rate. Our observations suggested that triticale shows promise for use in the management of crucifer pests.
High intensive shortwave light can be obtained through triboelectrification induced triboplasma discharge in sliding friction. Therefore, It will present a new method for emitting shortwave light which can be used as light source to control agricultural pests by wind-driven sliding friction without extra energy supply. The intensity of shortwave emission from triboplasma discharge induced by triboelectrification in reciprocating rubbing movement between PTFE and quartz tube filled Ar-N2 mixed gas had be investigated. The spectrum of shortwave emission near UV and violet and especially 52282 counts light intensity at 337nm peak wavelength was obtained. Further, the double-cylindrical shape of quartz tube was designed to form the triboplasma light emission device. The vertical shaft wind turbine was used to constitute the rotating driving part to satisfy the self-powered application needs.This work provides a novel method to emit shortwave emission for light trapping agricultural pests to control crop/forage grass pest-insects without pesticides by utilizing windy energy in the vast field or wild grassland.
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