Age-specific digestion of Tenebrio molitor (Coleoptera: Tenebrionidae) and inhibition of proteolytic and amylolytic activity by plant proteinaceous seed extracts

Dastranj, Mehdi; Bandani, Ali Reza; Mehrabadi, Mohammad

doi:10.1016/j.aspen.2013.04.009

Cited by 20 publications

(13 citation statements)

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“…The effects of A. sativa inhibitor on α-amylase of S. cerealella indicated a dose dependent manner of inhibition. These findings are in agreement with other reports; for example, Dastranj et al (2013) noted that 14 μg of crude protein of wheat (cultivar MV17) caused 71% inhibition of amylolytic activity in T. molitor , whereas the lowest concentration (0.87 μg of crude protein) inhibited only 18% of amylolytic activity. Electrophoretic resolution of the enzyme inhibition also showed that the activity of α-amylase decreased as a dose dependent manner of inhibition.…”

Section: Discussionsupporting

confidence: 93%

“…This enzyme converts starch to maltose, which is then hydrolyzed to glucose by α-glucosidase ( Kazzazi et al 2005 , Mohammadzadeh and Izadi 2016 ). Stored-product insects like Trogoderma granarium (Everts; Coleoptera: Dermestidae), Tenebrio molitor (L.; Coleoptera, Tenebrionidae), Prostephanus truncates (Horn; Coleoptera: Bostrychidae) and S. cerealella are extensively starch dependent species and require α-amylase for their development and survival ( Mendiola-Olaya et al 2000 ; Dastranj et al 2013 ; Borzoui et al 2015 , 2017 ). Therefore, the inhibition of this enzyme can be targeted in the development of new insecticidal technologies ( Silva et al 2001 ).…”

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confidence: 99%

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In Vitro and In Vivo Effects of α-Amylase Inhibitor From Avena sativa Seeds on Life History and Physiological Characteristics of Sitotroga cerealella (Lepidoptera: Gelechiidae)

Borzoui

Nouri-Ganbalani

Naseri

2017

Journal of Insect Science

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The inhibitory effects of Avena sativa L. seed extract were studied on life history and some physiological aspects of Sitotroga cerealella (Olivier; Lepidoptera: Gelechiidae). The inhibition of α-amylase activity in vitro by A. sativa proteinaceous extract suggested its potential antimetabolic effect on S. cerealella larvae. Although, chronic ingestion of A. sativa inhibitor (I10: 0.108 mg protein/artificial seed) did not show significant reduction of the growth and development of S. cerealella. However, a delay in the developmental time of immature stages was detected when S. cerealella larvae were continuously fed on I30 and I50 concentrations (0.429 and 1.11 mg protein/artificial seed, respectively) of the inhibitor. The highest realized fecundity was recorded for females which came from larvae fed on I10 concentration (102.46 ± 2.50 eggs/female), and the lowest fecundity was observed for females which came from larvae fed on I50 concentration (31.64 ± 3.17 eggs/female). The lightest weight of pupae of S. cerealella was observed on I50 concentration (2.76 ± 0.07 mg). The lowest glycogen and lipid contents of the pupae were detected on I50 concentration (50.00 ± 3.53 and 289.57 ± 29.00 µg/pupa, respectively). The lower survival rate of pupae at low temperature indicated that S. cerealella fed on I50 concentration of the inhibitor was less cold tolerant than control insects. The inhibitory studies indicated that A. sativa proteinaceous extract is a good candidate as an inhibitor of the α-amylase of this pest. This inhibitor can be expressed in genetically engineered plants to confer resistance to S. cerealella.

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Section: Discussionsupporting

confidence: 93%

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confidence: 99%

In Vitro and In Vivo Effects of α-Amylase Inhibitor From Avena sativa Seeds on Life History and Physiological Characteristics of Sitotroga cerealella (Lepidoptera: Gelechiidae)

Borzoui

Nouri-Ganbalani

Naseri

2017

Journal of Insect Science

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“…Their results showed the coffee berry borer α-amylase activity was inhibited substantially and at relatively low levels of the amylase inhibitor from the common bean and much less so by the amylase inhibitor from Amaranthus. In another study, metabolites extracted from different wheat cultivars showed varying specificity toward α-amylase of Tenebrio molitor L. (Dastranj et al, 2013). They found at concentration of 14 μg protein from seed extract of the different wheat cultivars including MV17, Aflak, sivand, saymon, zare inhibited the α-amylase activity by 58.3, 56.2, 58.5, 57.2, and 48.5, respectively.…”

Section: Discussionmentioning

confidence: 93%

Xanthogalerucella luteola (Col.: Chrysomelidae) α-amylase affected by seed proteinaceous extract from datura, wild oat and amaranth seeds

Esmaeily¹,

Bandani²

2016

jcp

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The elm leaf beetle, Xanthogalerucella luteola (Muller) (Col.: Chrysomelidae) is a serious pest of elm trees and it has been distributed all over the world. The current study was undertaken to investigate the inhibitory effects of protein extracts of three weed seeds including datura Datura stramonium L., amaranth Amaranthus retroflexus L. and wild oat Avena fatua L. against X. luteola α-amylase using spectrophotometric assay as well as in gel assays. The effects of five concentrations of each seed proteinaceous extracts were tested on α-amylase activity of the larval gut. The results showed a dose dependent manner in inhibition of the insect enzyme. At the highest concentration of protein extracts (12 μg protein) of all three seed extracts including amaranth, wild oat and datura, the inhibition was 71, 79 and 31%, respectively. Whilst, at low concentration (0.75 μg protein), the inhibition observed was 15, 36 and 5%, respectively. Thus, the greatest inhibition percentage was obtained when proteinaceous extract of wild oat seed was used. These results were confirmed when in gel assays were performed. All three seed proteinaceous extracts had an optimum pH inhibition of 6.0. Thus, it is concluded that wild oat seed proteins are potentially good for detailed investigation in order to get a clear picture of its active compound/s and its structure-function relationship.

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“…In their assays the lowest concentration of wheat and triticale seed extracts (0.106 mg protein/ml) inhibited 39% and 18% and the highest concentration (1.7 mg protein/ml) inhibited 82% and 75% of the amylase activity, respectively. Dastranj et al (2013) stated that bean, and wheat cultivars; MV17, Aflak, Sivand, Saymon, and Zare inhibited the α-amylase activity of T. molitor with the percentage of 70.9, 58.3,56.2, 58.5, 57.2, and 48.5, respectively. Most of the results in current study were in accordance with mentioned literature.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of barley and wheat seed protein on digestive α-amylase and general protease activity of Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae)

Pourabad

Dastranj

Ashouri

et al. 2015

Turkish Journal of Entomology

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Age-specific digestion of Tenebrio molitor (Coleoptera: Tenebrionidae) and inhibition of proteolytic and amylolytic activity by plant proteinaceous seed extracts

Cited by 20 publications

References 41 publications

In Vitro and In Vivo Effects of α-Amylase Inhibitor From Avena sativa Seeds on Life History and Physiological Characteristics of Sitotroga cerealella (Lepidoptera: Gelechiidae)

In Vitro and In Vivo Effects of α-Amylase Inhibitor From Avena sativa Seeds on Life History and Physiological Characteristics of Sitotroga cerealella (Lepidoptera: Gelechiidae)

Xanthogalerucella luteola (Col.: Chrysomelidae) α-amylase affected by seed proteinaceous extract from datura, wild oat and amaranth seeds

Inhibitory effects of barley and wheat seed protein on digestive α-amylase and general protease activity of Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae)

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