Salivary Extracellular Vesicle-Associated exRNA as Cancer Biomarker

Chiabotto, Giulia; Gai, Chiara; Deregibus, Maria Chiara; Camussi, Giovanni

doi:10.3390/cancers11070891

Cited by 46 publications

(37 citation statements)

References 185 publications

(214 reference statements)

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“…Blood plasma has now overtaken serum-containing medium as the second most-used source of EVs [12]. Another biofluid, saliva, was not registered in the previous survey but is now studied by 6% of respondents, in line with recent indications of its versatility in biomarker applications [13,14]. Overall, the growing number of researchers sourcing EVs from biofluids relative to cell culture suggests a shift of the EV field towards more in vivo studies and clinical assays.…”

Section: Conclusion and Final Remarksmentioning

confidence: 97%

Methods for Separation and Characterization of Extracellular Vesicles: Results of a Worldwide Survey Performed by the ISEV Rigor and Standardization Subcommittee

Royo

Théry

Falcón‐Pérez

et al. 2020

Cells

266

226

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Research on extracellular vesicles (EVs) is growing exponentially due to an increasing appreciation of EVs as disease biomarkers and therapeutics, an expanding number of EV-containing materials under study, and application of new preparation, detection, and cargo analysis methods. Diversity of both sources and methodologies imposes challenges on the comparison of measurement results between studies and laboratories. While reference guidelines and minimal requirements for EV research have achieved the important objective of assembling community consensus, it is also essential to understand which methodologies and quality controls are currently being applied, and how usage trends are evolving. As an initial response to this need, the International Society for Extracellular Vesicles (ISEV) performed a worldwide survey in 2015 on “Techniques used for the isolation and characterization of extracellular vesicles” and published the results from this survey in 2016. In 2019, a new survey was performed to assess the changing state of the field. The questionnaire received more than 600 full or partial responses, and the present manuscript summarizes the results of this second worldwide survey. The results emphasize that separation methods such as ultracentrifugation and density gradients are still the most commonly used methods, the use of size exclusion chromatography has increased, and techniques based on tangential flow and microfluidics are now being used by more than 10% of respondents. The survey also reveals that most EV researchers still do not perform sample quality controls before or after isolation of EVs. Finally, the majority of EV researchers emphasize that separation and characterization of EVs should receive more attention.

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Section: Conclusion and Final Remarksmentioning

confidence: 97%

Methods for Separation and Characterization of Extracellular Vesicles: Results of a Worldwide Survey Performed by the ISEV Rigor and Standardization Subcommittee

Royo

Théry

Falcón‐Pérez

et al. 2020

Cells

266

226

View full text Add to dashboard Cite

show abstract

“…Interest in the application of saliva-Exos as potential diagnostic and prognostic biomarkers of cancer is increasing [15]. Few studies have, however, focused on the therapeutic effects of saliva-Exos.…”

Section: Discussionmentioning

confidence: 99%

Saliva exosomes-derived UBE2O mRNA promotes angiogenesis in cutaneous wounds by targeting SMAD6

Chen

Xiong

et al. 2020

J Nanobiotechnol

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Background: Enhancing angiogenesis is critical for accelerating wound healing. Application of different types of exosomes (Exos) to promote angiogenesis represents a novel strategy for enhanced wound repair. Saliva is known to accelerate wound healing, but the underlying mechanisms remain unclear. Results: Our results have demonstrated that saliva-derived exosomes (saliva-Exos) induce human umbilical vein endothelial cells (HUVEC) proliferation, migration, and angiogenesis in vitro, and promote cutaneous wound healing in vivo. Further experiments documented that Ubiquitin-conjugating enzyme E2O (UBE2O) is one of the main mRNAs of saliva-Exos, and activation of UBE2O has effects similar to those of saliva-Exos, both in vitro and in vivo. Mechanistically, UBE2O decreases the level of SMAD family member 6 (SMAD6), thereby activating bone morphogenetic protein 2 (BMP2), which, in turn, induces angiogenesis. Conclusions: The present work suggests that administration of saliva-Exos and UBE2O represents a promising strategy for enhancing wound healing through promotion of angiogenesis.

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“…Interest in the application of saliva-Exos as potential diagnostic and prognostic biomarkers of cancer is increasing. [15] Few studies have, however, focused on the therapeutic effects of saliva-Exos. The present study demonstrated that treatment of C57/BL6 mice with saliva-Exos promotes wound healing.…”

Section: Discussionmentioning

confidence: 99%

Saliva Exosomes-Derived UBE2O Promotes Angiogenesis in Cutaneous Wounds By Targeting SMAD6

Chen

Xiong

et al. 2020

SSRN Journal

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Salivary Extracellular Vesicle-Associated exRNA as Cancer Biomarker

Cited by 46 publications

References 185 publications

Methods for Separation and Characterization of Extracellular Vesicles: Results of a Worldwide Survey Performed by the ISEV Rigor and Standardization Subcommittee

Methods for Separation and Characterization of Extracellular Vesicles: Results of a Worldwide Survey Performed by the ISEV Rigor and Standardization Subcommittee

Saliva exosomes-derived UBE2O mRNA promotes angiogenesis in cutaneous wounds by targeting SMAD6

Saliva Exosomes-Derived UBE2O Promotes Angiogenesis in Cutaneous Wounds By Targeting SMAD6

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