Salivary protein adsorption onto hydroxyapatite and sds‐mediated elution studied byin situellipsometry

Validation of mechanically-assisted sodium dodecyl-sulphate elution as a technique to remove pellicle protein components from human enamel

“…Previous in situ studies on the removal of salivary films from HA showed incomplete removal when the salivary film was subjected to rinsing with a 0.5% SDS solution (Hahn Berg et al 2001). The results presented here show that 0.5% SDS was sufficient to completely remove the pellicle, and it appears that mechanical rubbing with fibre pellets had a large influence on the removal of adsorbed proteins.…”

“…In this particular case, when dealing with enamel surfaces that have been reported to be negatively charged (Arends and Jongebloed 1977;Arends 1979), SDS is expected to form complexes with adsorbed proteins, which then are electrostatically repelled from the surface. The concentration interval of SDS was chosen based on previous pellicle collection studies (Carle´n et al 1998;Hahn Berg et al 2001;Yao et al 2001;Hannig et al 2005) and on the fact that the critical micelle concentration (cmc) of SDS in water is known to be 8.3 mM (0.25% w/v). The results indicate that there is a critical concentration at approximately 26 cmc (0.5% SDS) that completely desorbs the pellicle.…”

“…Yao et al (2001) evaluated several different eluents and showed that, among these, SDS was the most efficient, but still only desorbed approximately 80% of the pellicle proteins from hydroxyapatite (HA) at a concentration of 0.1%. Further, SDS has also been used extensively for elution of in situ-formed salivary films where a solution of 0.5% SDS completely removed adsorbed salivary films on hydrophilic silica, whereas some residual material was left on HA surfaces (Hahn Berg et al 2001). Hannig et al (2005) investigated the removal of in situ-formed pellicles on wet-ground and polished bovine incisors and showed that the pellicle was incompletely removed by polyurethane foam sponges soaked in 2% SDS.…”

“…Due to contradictory statements in the literature on the pellicle removal efficiency of SDS on different types of surfaces, using various techniques for visualisation of the results, including one-dimensional gel electrophoresis (1-DE) (Carle´n et al 1998;Milleding et al 2001;Yao et al 2001, Hannig et al 2005, immunoblotting (Carle´n et al 1998;Milleding et al 2001), densiometry (Yao et al 2001), ellipsometry (Hahn Berg et al 2001), atomic force microscopy (Milleding et al 2001) and transmission electron microscopy (Hannig et al 2005), the aim of the present study was to investigate the effectiveness of SDS in eluting both in vivo and in vitro pellicles formed on natural human teeth, which had not been subjected to any treatment other than pumicing.…”

“…The pellicle possesses many important functions such as maintaining tooth integrity by controlling the mineral solution dynamics of the enamel, and having tissue-coating, antimicrobial and lubricating abilities. Since this is of importance for oral health and comfort, there has been a great deal of interest in characterising the composition of salivary pellicles over the years (Lendenmann et al 2000;Hannig and Joiner 2006;Deimling et al 2007;Siqueira et al 2007;Vitorino et al 2007) For these investigations, different techniques were applied for the removal of pellicles (Carle´n et al 1998;Hahn Berg et al 2001;Yao et al 2001;Hannig et al 2005) where sodium dodecyl sulphate (SDS) has been used frequently as eluent at different concentrations. Carle´n et al (1998) developed a technique for in vivo pellicle elution using a 2% SDS solution, which although not validated on human enamel surfaces, was shown to completely desorb surface-bound proteins on modified gold surfaces (Ka¨lltorp et al 2000).…”