Validation of mechanically-assisted sodium dodecyl-sulphate elution as a technique to remove pellicle protein components from human enamel

Svendsen, Ida; Arnebrant, Thomas; Lindh, Liselott

doi:10.1080/08927010802018277

Cited by 14 publications

(18 citation statements)

References 24 publications

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“…The technique based on mechanically assisted SDS elution of adsorbed proteins has been used in several previous publications and shown to be efficient in removal of different types of protein films (Santos et al 2011, Carlen et al 1998, Kalltorp et al 2000, Milleding et al 2001, Svendsen et al 2008. Quantification using gel electrophoresis in combination with Sypro Ruby staining is also a technique used in the literature (Berggren et al 2000, Chevalier et al 2004, Wheelock et al 2006) and Sypro staining is known to be very sensitive with linearity over 3 orders of magnitude, making it one of the most trustworthy stains for quantifying proteins in gel electrophoresis.…”

Section: Adsorbed Proteins From Protein Mixture Determined By Gel Elementioning

confidence: 99%

Adsorption of HSA, IgG and laminin-1 on model hydroxyapatite surfaces – effects of surface characteristics

et al. 2012

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Ellipsometry and mechanically assisted sodium dodecyl sulphate elution was utilized to study the adsorption of human serum albumin (HSA), human immunoglobulin G (IgG), laminin-1, as well as competitive adsorption from a mixture of these proteins on spin-coated and sintered hydroxyapatite (HA) surfaces, respectively. The HA surfaces were characterized concerning wettability and roughness by means of water contact angles and atomic force microscopy, respectively. Both surface types were hydrophilic, and the average roughness (Sa) and surface enlargement (Sdr) were lower for the sintered compared to the spin-coated HA surfaces. The adsorbed amounts on the sintered HA increased as follows: HSA < laminin-1 < IgG < protein mixture. For the competitive adsorption experiments, the adsorbed fractions increased accordingly: HSA < laminin-1 < IgG on both types of HA substrata. However, a higher relative amount of HSA and laminin-1 and a lower relative amount of IgG was found on the spin-coated surfaces compared to on the sintered ones.The effects observed could be ascribed to differences in surface roughness and chemical composition between the two types of HA substrata, and could have an influence on future implant surface coatings.

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Section: Adsorbed Proteins From Protein Mixture Determined By Gel Elementioning

confidence: 99%

Adsorption of HSA, IgG and laminin-1 on model hydroxyapatite surfaces – effects of surface characteristics

et al. 2012

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“…During the patient's second visit, paper Sialostrips TM (Periotron; Oraflow Inc., New York, N.Y., USA) were applied to lingual surfaces of the upper incisors for 5 s for collection of a salivary film sample and volume estimated by electrical conductance [Pramanik et al, 2010]. Then enamel pellicle was collected from the dried incisor tooth by rubbing the lingual surface of the upper incisors with SDS (0.5% w/v)-soaked filter paper, as described previously [Svendsen et al, 2008]. The splint was then worn for 1 h (without eating, drinking or talking).…”

Section: Preparation Of the Splintsmentioning

confidence: 99%

Composition of Enamel Pellicle from Dental Erosion Patients

Carpenter¹,

Cotroneo²,

Moazzez³

et al. 2014

Caries Res

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Oral health is dependent upon a thin mobile film of saliva on soft and hard tissues. Salivary proteins adhere to teeth to form the acquired enamel pellicle which is believed to protect teeth from acid erosion. This study investigated whether patients suffering diet-induced dental erosion had altered enamel pellicles. Thirty patients suffering erosion were compared to healthy age-matched controls. Subjects wore a maxillary splint holding hydroxyapatite and human enamel blocks for 1 h. The acquired enamel pellicle was removed from the blocks and compared to the natural incisor pellicle. Basic Erosive Wear Examination scores confirmed that dental erosion was present in erosion patients and absent from healthy age-matched controls. Erosion patients had half the amount of proteins (BCA assay) within the acquired pellicle forming on splint blocks compared to normal controls (p < 0.05). In particular, statherin, a calcium-binding protein, was 35% less abundant (p < 0.05). Calcium concentration within the acquired pellicle was also reduced by 50% in erosion patients (p < 0.001). In contrast, the natural pellicle on the incisor had similar amounts of total protein in erosion patients and healthy controls. In summary, the formation of new acquired pellicles on surfaces was reduced in erosion patients, which may explain their greater susceptibility to acid erosion of teeth.

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“…For this, a mechanically assisted sodium dodecyl sulphate (SDS) elution procedure was employed. This method, which consists in rubbing surfaces with polyamide fibre pellets soaked in SDS solution, has been verified to be a reliable one for complete pellicle desorption (Svendsen et al 2008). Several approaches are available for characterizing the proteinaceous composition of the pellicle, including electrophoretic, immunological, histochemical and chromatographic techniques (Hannig & Joiner 2006).…”

Section: Introductionmentioning

confidence: 99%

“…First, the pellicle has to be collected. Improved harvesting techniques have recently been introduced, eg combining mechanical and chemical collecting methods (Carlén et al 1998;Yao et al 2001;Svendsen et al 2008). For this, a mechanically assisted sodium dodecyl sulphate (SDS) elution procedure was employed.…”

Section: Introductionmentioning

confidence: 99%

Influence of substratum hydrophobicity on salivary pellicles: organization or composition?

Aroonsang¹,

Sotres²,

El-Schich³

et al. 2014

Biofouling

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Different physico-chemical properties (eg adsorption kinetics, thickness, viscoelasticity, and mechanical stability) of adsorbed salivary pellicles depend on different factors, including the properties (eg charge, roughness, wettability, and surface chemistry) of the substratum. Whether these differences in the physico-chemical properties are a result of differences in the composition or in the organization of the pellicles is not known. In this work, the influence of substratum wettability on the composition of the pellicle was studied. For this purpose, pellicles eluted from substrata of different but well-characterized wettabilities were examined by means of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that substratum hydrophobicity did not have a major impact on pellicle composition. In all substrata, the major pellicle components were found to be cystatins, amylases and large glycoproteins, presumably mucins. In turn, interpretation of previously reported data based on the present results suggests that variations in substratum wettability mostly affect the organization of the pellicle components.

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Validation of mechanically-assisted sodium dodecyl-sulphate elution as a technique to remove pellicle protein components from human enamel

Cited by 14 publications

References 24 publications

Adsorption of HSA, IgG and laminin-1 on model hydroxyapatite surfaces – effects of surface characteristics

Adsorption of HSA, IgG and laminin-1 on model hydroxyapatite surfaces – effects of surface characteristics

Composition of Enamel Pellicle from Dental Erosion Patients

Influence of substratum hydrophobicity on salivary pellicles: organization or composition?

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