Salivary proteome characterization of alcohol and tobacco dependents

Batista, Thiago Beltrami Dias; Chaiben, Cassiano Lima; Penteado, Carlos Antonio Schäffer; Nascimento, Júlia Milena Carvalho; Ventura, Talita Mendes Oliveira; Dionízio, Aline; Rosa, Edvaldo Antônio Ribeiro; Buzalaf, Marília Afonso Rabelo; Azevedo‐Alanis, Luciana Reis

doi:10.1016/j.drugalcdep.2019.06.013

Cited by 18 publications

(11 citation statements)

References 39 publications

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“…Still, saliva sampling can be impeding in patients suffering from xerostomia due to radiotherapy, surgery of the salivary glands, or psychological stress. Salivary flow and composition are further affected by circadian rhythm and stress ( 37 ), food uptake, gingival bleeding, or alcohol and tobacco consumption ( 38 ). Even alcohol-/tobacco-induced changes in the RNA profile of airway epithelial cell EVs have been described ( 39 ), but the influence of tobacco/alcohol on EV composition in oral cancer is rarely studied ( 40 ).…”

Section: Discussionmentioning

confidence: 99%

Cargo and Functional Profile of Saliva-Derived Exosomes Reveal Biomarkers Specific for Head and Neck Cancer

Hofmann¹,

Medyany²,

Ezić³

et al. 2022

Front. Med.

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BackgroundExosomes contribute to immunosuppression in head and neck squamous cell carcinoma (HNSCC), a tumor entity which lacks specific tumor biomarkers. Plasma-derived exosomes from HNSCC patients correlate with clinical parameters and have potential as liquid biopsy. Here, we investigate the cargo and functional profile of saliva-derived exosomes from HNSCC patients and their potential as non-invasive biomarkers for disease detection and immunomodulation.MethodsExosomes were isolated from saliva of HNSCC patients (n = 21) and healthy donors (HD, n = 12) by differential ultracentrifugation. Surface values of immune checkpoints and tumor associated antigens on saliva-derived exosomes were analyzed by bead-based flow cytometry using CD63 capture. Upon co-incubation with saliva-derived exosomes, activity and proliferation of T cells were assessed by flow cytometry (CD69 expression, CFSE assay). Adenosine levels were measured by mass spectrometry after incubation of saliva-derived exosomes with exogenous ATP. miRNA profiling of saliva-derived exosomes was performed using the nCounter® SPRINT system.ResultsSaliva-derived, CD63-captured exosomes from HNSCC patients carried high amounts of CD44v3, PDL1 and CD39. Compared to plasma, saliva was rich in tumor-derived, CD44v3+ exosomes and poor in hematopoietic cell-derived, CD45+ exosomes. CD8+ T cell activity was attenuated by saliva-derived exosomes from HNSCC patients, while proliferation of CD4+ T cells was not affected. Further, saliva-derived exosomes produced high levels of immunosuppressive adenosine. 62 HD- and 31 HNSCC-exclusive miRNAs were identified. Samples were grouped in “Healthy” and “Cancer” based on their saliva-derived exosomal miRNA profile, which was further found to be involved in RAS/MAPK, NF-κB complex, Smad2/3, and IFN-α signaling.ConclusionsSaliva-derived exosomes from HNSCC patients were enriched in tumor-derived exosomes whose cargo and functional profile reflected an immunosuppressive TME. Surface values of CD44v3, PDL1 and CD39 on CD63-captured exosomes, adenosine production and the miRNA cargo of saliva-derived exosomes emerged as discriminators of disease and emphasized their potential as liquid biomarkers specific for HNSCC.

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Section: Discussionmentioning

confidence: 99%

Cargo and Functional Profile of Saliva-Derived Exosomes Reveal Biomarkers Specific for Head and Neck Cancer

Hofmann¹,

Medyany²,

Ezić³

et al. 2022

Front. Med.

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“…To control for potential effects of energy level, the quantity of each snack consumed was adjusted to get equal energy content. Moreover, since different factors can affect saliva composition, such as sex and BMI [ 13 ], as well as smoking and alcohol habits [ 27 ], only normal-weight non-smoker and non-alcohol consumers were included.…”

Section: Discussionmentioning

confidence: 99%

How Different Snacks Produce a Distinct Effect in Salivary Protein Composition

et al. 2021

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Saliva secretion changes in response to different stimulation. Studies performed in animals and humans suggest that dietary constituents may influence saliva composition, although the dynamics of these changes, and how they are specific for each type of food, are little known. The objective of the present study was to access the short-term effects of different foods in salivation and salivary protein composition. Twelve participants were tested for four snacks (yoghurt, bread, apple and walnuts). Non-stimulated saliva was collected before and at 0′, 5′ and 30′ after each snack intake. Flow rate, total protein, alpha-amylase enzymatic activity and salivary protein profile were analyzed. Yoghurt and apple were the snacks resulting in higher salivary changes, with higher increases in flow rate and alpha-amylase activity immediately after intake. The expression levels of immunoglobulin chains decreased after the intake of all snacks, whereas cystatins and one pink band (proline-rich proteins—PRPs) increased only after yoghurt intake. Walnut’s snack was the one resulting in lower changes, probably due to lower amounts eaten. Even so, it resulted in the increase in one PRPs band. In conclusion, changes in saliva composition varies with foods, with variable changes in proteins related to oral food processing and perception.

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“…However, in this study, the protein extraction and proteomic analysis protocols followed a recently developed methodology that increases the identification of proteins in in vivo acquired enamel pellicle samples [22]. We highlight that the analysis of pooled samples has been widely used in proteomic studies involving quantitative analysis of acquired pellicle [20,22,[25][26][27][28][29][30]. Moreover, in this study, the proteins were individually extracted, and a biological triplicate was done.…”

Section: Discussionmentioning

confidence: 99%

Proteomic profile of the acquired enamel pellicle of professional wine tasters with erosive tooth wear

Silva

Ventura

Oliveira

et al. 2021

European J Oral Sciences

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The aim of this study was to compare the acquired enamel pellicle protein profile of professional wine tasters with mild and moderate erosive tooth wear. Twelve professional wine tasters participated (3 from a low tooth wear group; 9 from a high tooth wear group). Acquired enamel pellicle samples were collected and processed for proteomic analysis (nLC-ESI-MS/MS). The acquired enamel pellicle proteomic profile was different between the groups. The proteins found exclusively in the low

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Salivary proteome characterization of alcohol and tobacco dependents

Cited by 18 publications

References 39 publications

Cargo and Functional Profile of Saliva-Derived Exosomes Reveal Biomarkers Specific for Head and Neck Cancer

Cargo and Functional Profile of Saliva-Derived Exosomes Reveal Biomarkers Specific for Head and Neck Cancer

How Different Snacks Produce a Distinct Effect in Salivary Protein Composition

Proteomic profile of the acquired enamel pellicle of professional wine tasters with erosive tooth wear

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