Salivary tissue factor concentration and activity in patients with oral lichen planus

Tunalı-Akbay, Tuğba; Solmaz, Zeliha; Pekiner, Filiz Namdar; Ipekci, Hazal

doi:10.1016/s1348-8643(16)30017-9

Cited by 4 publications

(3 citation statements)

References 33 publications

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“…Corroborating the ndings of our study, Tugba Tunali-Akbay et al demonstrated that total antioxidant capacity (TAC) and GSH accumulation were lower in patients with OLP (30). In addition to our results, Daniela Miricescu et al illustrated that OS reduced the antioxidant levels in the oral cavity.…”

Section: Discussionsupporting

confidence: 88%

Evaluation of the salivary level of Glutathione reductase, Catalase and Free thiol in patients with oral lichen plaus

Rezazadeh

Khorami

Mahdavi

et al. 2023

Preprint

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Objective: Oral lichen planus (OLP) is a usual chronic inflammatory disease of the oral mucosa with malignant capacity, whose pathogenesis is not yet well known. Free radicals and reactive oxygen species may have a vital role in the pathogenesis of oral lichen planus. This study aimed to assess Glutathione reductase, catalase, and free thiol levels in the saliva of OLP patients and compare it with healthy people. Methods: In this cross-sectional study, 35 patients with OLP and 20 healthy people were involved. Five mL of whole, unstimulated saliva samples were collected in the morning, and the salivary levels of antioxidants were measured by ELISA technique. In this experiment, sex, age and OLP types were also evaluated. Results: There was a significant decrease in the salivary level of Glutathione reductase and catalase in patients (p=0.001), but there were no differences between the two groups regarding free-thiol level (p=0.7). However, there was no correlation between age and gender with the antioxidants’ contents. There was a significant decrease in glutathione reductase and catalase in the erosive type than in the non-erosive type. Conclusions: In this study, we found that the salivary levels of Glutathione reductase and Catalase were lower in OLP patients than in the healthy group, which means these antioxidants were affected by OLP and also related to the type of it. So salivary Glutathione reductase and Catalase levels may be used as biomarkers for OLP monitoring and treatment.

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Section: Discussionsupporting

confidence: 88%

Evaluation of the salivary level of Glutathione reductase, Catalase and Free thiol in patients with oral lichen plaus

Rezazadeh

Khorami

Mahdavi

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Corroborating the findings of our study, Tugba Tunali-Akbay et al demonstrated that total antioxidant capacity (TAC) and GSH accumulation were lower in patients with OLP [ 28 ]. In addition to our results, Daniela Miricescu et al illustrated that OS reduced the antioxidant levels in the oral cavity.…”

Section: Discussionsupporting

confidence: 88%

Evaluation of the salivary level of glutathione reductase, catalase and free thiol in patients with oral lichen planus

et al. 2023

View full text Add to dashboard Cite

Objective Oral lichen planus (OLP) is a usual chronic inflammatory disease of the oral mucosa with malignant capacity, whose pathogenesis is not yet well known. Free radicals and reactive oxygen species may have a vital role in the pathogenesis of oral lichen planus. This study aimed to assess Glutathione reductase, catalase, and free thiol levels in the saliva of OLP patients and compare it with healthy people. Methods In this cross-sectional study, 35 patients with OLP and 20 healthy people were involved. Five mL of whole, unstimulated saliva samples were collected in the morning, and the salivary levels of antioxidants were measured by ELISA technique. In this experiment, sex, age and OLP types were also evaluated. Results There was a significant decrease in the patients’ salivary level of Glutathione reductase (0.2043 mU/ml in patients and 0.3901 mU/ml in the control group) and catalase (0.1525 mU/ml in patients and 0.2700 mU/ml in the control group) (p = 0.001). But there were no differences between the two groups regarding free-thiol levels (0.0586 mU/ml in patients and 0.0569 mU/ml in the control group) (p = 0.7). However, there was no correlation between age and gender with the antioxidants’ contents. There was a significant decrease in glutathione reductase and catalase in the erosive type than in the non-erosive type. Conclusions In this study, we found that the salivary levels of Glutathione reductase and Catalase were lower in OLP patients than in the healthy group, which means these antioxidants were affected by OLP and also associated with the type of it. So salivary Glutathione reductase and Catalase levels may be used as biomarkers for OLP monitoring and treatment.

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“…Based on comparison of TG curves induced by saliva or r-TF, we estimated the concentration of TF in saliva to be in the order of 1-2 ng/mL, consistent with literature. 5,12 To confirm that TF was indeed the procoagulant factor in saliva that triggered TG, we compared TG from the same saliva sample (from five donors) in the absence and presence of anti-TF antibodies ( Figure 1B). Although thrombin was still generated in the presence of the antibodies, the lag time of this TG curve was substantially prolonged.…”

Section: Resultsmentioning

confidence: 99%

Salivary tissue factor induces thrombin generation in a diurnal rhythm

Vorm

Brouwers²,

Mondria

et al. 2018

Research and Practice in Thrombosis and Haemostasis

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BackgroundUpon tooth extraction, extravascular tissue factor (TF) initiates coagulation to arrest bleeding. Additionally, saliva is in constant contact with the wound and contains extracellular vesicle‐derived procoagulant TF. Since the duration of postextraction bleeding is highly variable between patients, we hypothesized this may be caused by variation in saliva‐derived TF‐induced clotting activity.ObjectivesWe aimed to assess the variability of saliva‐induced thrombin generation (TG) in healthy individuals.Methods TG was performed according to the calibrated automated thrombinography (CAT) method. Diluted saliva was added (instead of recombinant TF and phospholipids [PL]) to normal pooled plasma (NPP) in the absence/presence of anti‐TF antibodies. Saliva was collected from healthy individuals in the morning, afternoon and evening.ResultsAddition of saliva to NPP induced TG curves similar to those induced by r‐TF and PL. Moreover, addition of anti‐TF antibodies abolished saliva‐induced TG, indicating TF‐dependence. A large inter‐individual variability (peak CV 31%, range 73‐220 nmol/L thrombin) in saliva‐induced TG was observed. Interestingly, within subjects, saliva‐induced TG was significantly (P = 0.009) increased in the morning (167 ± 40 nmol/L thrombin) compared to the afternoon (124 ± 39 nmol/L thrombin) and evening (123 ± 38 nmol/L thrombin). This diurnal variation was not attributable to gingival stimulation or damage induced by tooth brushing.ConclusionsWe identified a diurnal rhythm in salivary TF activity that may have implications for tooth extraction and dental surgery, as performing invasive procedures in the morning may be beneficial for rapid coagulation. Future studies should correlate salivary TF to clinical outcome (ie, postextraction bleeding) and assess a possible relation with bacterial status in the oral cavity.

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Salivary tissue factor concentration and activity in patients with oral lichen planus

Cited by 4 publications

References 33 publications

Evaluation of the salivary level of Glutathione reductase, Catalase and Free thiol in patients with oral lichen plaus

Evaluation of the salivary level of Glutathione reductase, Catalase and Free thiol in patients with oral lichen plaus

Evaluation of the salivary level of glutathione reductase, catalase and free thiol in patients with oral lichen planus

Salivary tissue factor induces thrombin generation in a diurnal rhythm

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