Salivia Officinalis L. and Verbascum Phlomoides L. Chemical, Antimicrobial, Antioxidant and Antitumor Investigations

Marian, Eleonora; Vicaş, Laura; Jurca, Tünde; Mureşan, Mariana; Pallag, Annamária; Stan, Roxana Liana; Sevastre, Bogdan; Diaconeasa, Zorița; Ionescu, Clara M.; Hangan, Adriana Corina

doi:10.37358/rc.18.2.6108

Cited by 18 publications

(20 citation statements)

References 19 publications

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“…The calibration curve was linear for the range of Trolox concentrations between 0.125 and 2 mmol/L. The ABTS value was obtained using the following equation based on the calibration curve: y = 1629 x + 98.94 (R 2 = 0.998), where x = absorbance and y= µmol Trolox equivalent [32,33]. Trolox was used as a standard solution, the calibration curve was made for concentrations between 0 and 300 µM, having a correlation coefficient R 2 = 0.9956 and the regression equation (y =0.0017x+0.0848), where x = absorbance registered at 595 nm and y = µmol Trolox equivalent [34][35][36].…”

Section: Abts Methodsmentioning

confidence: 99%

Chemical Characterization of Artemisia Annua L. Extract Assessment of Antioxidant Activity in vitro and in vivo Toxicity Studies

Stan¹,

Sevastre²,

Hangan³

et al. 2019

Rev. Chim.

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The purpose of the study was to demonstrate in vitro antioxidant activity and in vivo toxicity of the Artemisia Annua L. extract. The plant was harvested from Bihor area (Crisul Repede and Negru river valleys), Romania. Preparation of the plant product and of the lyophilizated extract was carried out in accordance with the Romanian Pharmacopeia Xth Edition. Lyophilized extract was evaluated in terms of polyphenol content using HPLC method. Antioxidant activity was highlighted using the DPPH, ABTS and FRAP methods. Hepatic, renal and haematological toxicity studies have been performed on laboratory mice. For this purpose blood and organs were collected. Biochemical and haematological parameters were determined on the blood samples and histopathological examination was performed on organs. In vitro antioxidant effect of Artemisia Annua L extract and its lack of in vivo toxicity were demonstrated. It is desirable to obtain a new phytoproduct harvested from spontaneous flora of Romania with antioxidant / antitumoral properties and which is devoid of toxicity.

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Section: Abts Methodsmentioning

confidence: 99%

Chemical Characterization of Artemisia Annua L. Extract Assessment of Antioxidant Activity in vitro and in vivo Toxicity Studies

Stan¹,

Sevastre²,

Hangan³

et al. 2019

Rev. Chim.

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“…Trolox was used as a standard solution, the calibration curve was made for concentrations between 0 and 300 µM, having a correlation coefficient R 2 = 0.9956 and the regression equation (y =0.0017x+0.0848), where y represents absorbance detected at 595 nm. The results are expressed as µmol Trolox equivalents (TE)/100 mLextract [23,24].…”

Section: In Vitro Antioxidant Activity Dpph Radical Scavenging Assaymentioning

confidence: 99%

“…The scavenging ability (%) of the complexes was found 69±0. 23 FRAP method (ferric reducing antioxidant power)…”

Section: Dna Cleavagementioning

confidence: 99%

“…The in vivo SOD mimetic activity of (C 1 ) and (C 2 ) complexes was quantified by a method based on the protection against free radicals provided by the extracts to the yeast S. cerevisiae [23,26,52]. The SOD-mimetic activity of the complexes on cell growth with a Äsod1 mutant treated with menadione or H 2 O 2 had been evaluated.…”

Section: In Vivo Sod Mimetic Activitymentioning

confidence: 99%

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Synthesis, Characterization and Biological Activity of Two New Copper (II) Complexes with N-sulfonamide Ligand

Hangan¹,

Vicaş²,

Stan³

et al. 2019

Rev. Chim.

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Despite the fact that a large number of chemotherapeutic anticancer agents have been discovered, cancer still remains a great cause of deaths worldwide. The purpose of our researches is to discover a new antitumor drug. In this aim, two new Cu(II) complexes, [Cu(L)2(py)2(H2O)2](C1) and [Cu(L)2(phen)](C2) with a new ligand, N-(5-trifluoromethyl-[1,3,4]-thiadiazole-2-yl)-benzensulfonamide(HL) were synthesized. The complexes were characterized by elemental analysis, spectral and magnetic determinations. The nuclease activity studies of the complexes confirm their capacity to cleavage the DNA molecule. Both complexes have in vitro antioxidant activity (DPPH, FRAP methods), in vitro (using xanthine /xanthine oxidase system) and in vivo (using S.cerevisiae)SOD mimetic activity.The results of MTT assay on two carcinoma cell lines (HeLa and WM35) indicate that both complexes have antitumor activity, but (C2) has a superior activity compared with (C1) and with Cisplatin. On normal fibroblast (HDFa), (C1) showed toxicity comparable with Cisplatin, but (C2) showed a lower one. Bacterial assays were also performed (by the disk diffusion method) and both complexes have antibacterial activity against S. aureus, E. coli, P. aeroginosa and B. cereus. All the biological studies are in concordance and show that both complexes have biologic activity but (C2)is much more active.

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“…Medicinal herbs are important for the cancer treatment, due to their composition made of multiple chemical compounds, serving for the discovery of new active cytotoxic materials. In this context, our group of researchers has previously reported several studies demonstrating the in vitro/in vivo antioxidant and antitumour activities for Viscum album [27], Euonymus europaeus [23,24], Centaurea cyanus, Calendula officinalis [17], Salvia officinalis and Verbascum phlomoides [16]. The first objective of the present study was the artemisinin, quercetin and gallic acid evaluation using LC methods, from Artemisia annua L. extract, plant harvested from Romania, Bihor area.…”

Section: Introductionmentioning

confidence: 99%

Artemisia Annua L. Extract: A New Phytoproduct With Sod-Like and Antitumour Activity

Stan¹

2020

FARMACIA

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The purpose of the present study was to investigate the antitumour potential of Artemisia annua L., harvested from Romania, Bihor area. Artemisinin, quercetin and galic acid have been identified using the LC-MS method. Hypothesis that Artemisia annua L. antitumour effect relays on the modulation of antioxidant activity of SOD has been tested using the SOD defective S. cerevisiae model. According to our results, Artemisia annua L. revealed a selective inhibitory effect on tumour cells of WM35 melanoma cell line in vitro, with a calculated IC50 three times lower as compared to normal HDF fibroblasts. Also, in vivo studies on Ehrlich carcinoma model revealed the amplification effect of Artemisia annua L. on the cytostatic effect of doxorubicin. On both in vivo and in vitro models, the inhibition of the tumour cell proliferation was associated with inhibition of the activities of antioxidative enzymes activities, thus antitumour effect of Artemisia annua L. might be related to oxidative alterations in malignant cells. Rezumat Scopul studiului a fost de a investiga potențialul antitumoral al Artemisiei annua L., plantă recoltată din Romania, județul Bihor. Artemisinina, quercetina și acidul galic au fost identificate prin analiză LC-MS. Utilizând S. cerevisiae, am testat ipoteza că efectul antitumoral al Artemisia annua L. este corelat cu modularea activității antioxidante a SOD. In vitro, pe linia celulară de melanom (WM35), Artemisia annua L. a inhibat selectiv celulele tumorale, IC50 fiind de trei ori mai scăzut decât pentru fibroblastele normale (HDF). Studiile in vivo pe carcinomul ascitic Ehrlich au indicat faptul că Artemisia annua L. potențează efectul citostatic al doxorubicinei. Atât in vivo cât și in vitro, inhibarea proliferării tumorale a fost corelată cu inhibarea activității enzimelor antioxidante, astfel efectul antitumoral al Artemisia annua L. poate fi în legătură cu alterările oxidative de la nivelul celulelor tumorale.

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Salivia Officinalis L. and Verbascum Phlomoides L. Chemical, Antimicrobial, Antioxidant and Antitumor Investigations

Cited by 18 publications

References 19 publications

Chemical Characterization of Artemisia Annua L. Extract Assessment of Antioxidant Activity in vitro and in vivo Toxicity Studies

Chemical Characterization of Artemisia Annua L. Extract Assessment of Antioxidant Activity in vitro and in vivo Toxicity Studies

Synthesis, Characterization and Biological Activity of Two New Copper (II) Complexes with N-sulfonamide Ligand

Artemisia Annua L. Extract: A New Phytoproduct With Sod-Like and Antitumour Activity

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