Salmonella Gallinarum field isolates from laying hens are related to the vaccine strain SG9R

Immerseel, Filip Van; Studholme, David J.; Eeckhaut, Venessa; Heyndrickx, Marc; Dewulf, Jeroen; Dewaele, Isabelle; Hoorebeke, S. Van; Haesebrouck, Freddy; Meirhaeghe, H. van; Ducatelle, Richard; Paszkiewicz, Konrad; Titball, Richard W.

doi:10.1016/j.vaccine.2013.08.033

Cited by 41 publications

(44 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The acquisition of a more virulent phenotype by the rough vaccine strain 9R (reversion of virulence) was previously suggested by Van Immerseel et al (2013) in order to explain the genetic relatedness found, by PFGE, between the vaccine and some field strains. In this study, similar evidence was not supported by our data.…”

Section: Discussionmentioning

confidence: 94%

ERIC-PCR genotyping of field isolates ofSalmonella entericasubsp.entericaserovar Gallinarum biovars Gallinarum and Pullorum

et al. 2015

View full text Add to dashboard Cite

Salmonella Gallinarum (SG) and Salmonella Pullorum (SP) have been classified as biovars belonging to Salmonella enterica subsp. enterica serovar Gallinarum. Genetic diversity among isolates of the same biovar can be detected by DNA fingerprinting techniques which are useful in epidemiological investigations. In this study, we applied the PCR amplification of Enterobacterial Repetitive Intergenic Consensus sequences (ERIC-PCR) to analyse 45 strains of SG and SP, most of which were isolated from diseased poultry of different Brazilian regions over a period of 27 years until 2014. The ERIC-genotypes obtained were used to describe the epidemiological relationship amongst the strains. Our findings showed that there were six ERIC-patterns for SG strains at 80% similarity. In addition, some of the SG isolates recovered from different regions and years clustered with 100% similarity, suggesting that transfer of genotypes between these regions has taken place. The commercial rough vaccine strain 9R showed a unique profile. Meanwhile, more genetic diversity was observed among SP strains where ten ERICpatterns were also formed at 80% similarity.

show abstract

Section: Discussionmentioning

confidence: 94%

ERIC-PCR genotyping of field isolates ofSalmonella entericasubsp.entericaserovar Gallinarum biovars Gallinarum and Pullorum

et al. 2015

View full text Add to dashboard Cite

show abstract

“…For some time, great efforts have been made towards developing this type of vaccine with gene deletion because even though these are live salmonellas and therefore retain the field challenging behaviour during initial infection, they are also extremely safe as they do not persist in poultry or even in the environment for long periods of time (Hoiseth & Stocker, 1981;Barrow et al, 1990;Hormaeche et al, 1991;Cooper et al, 1994;Tan et al, 1997;Van Immerseel et al, 2002). Thus, the great advantage of genetically modified live vaccines is the low risk of reversion to virulence in comparison to vaccines made from attenuated rough strains, for example (Frey, 2007;Matsuda et al, 2010;Penha Filho et al, 2010;Matsuda et al, 2011a, b;Shehata et al, 2013;Van Immerseel et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the effectiveness and safety of a genetically modified live vaccine in broilers challenged with Salmonella Heidelberg

Muñíz¹,

Verdi²,

Leão³

et al. 2017

Avian Pathology

View full text Add to dashboard Cite

Salmonellosis ranks among the major diseases of commercial poultry, and its presence in poultry flocks is responsible for economic losses and risks related to public health. Vaccines are an important tool within integrated programmes to control salmonellosis. The purpose of this study was to assess cross-protection provided by the Poulvac® ST vaccine in the control of Salmonella Heidelberg in experimentally challenged 3-and 21-day-old birds. Eighty birds were identified and separated into four treatments (T1: vaccinated and challenged at 3 days of age, T2: unvaccinated and challenged at 3 days of age, T3: vaccinated and challenged at 21 days of age, and T4: unvaccinated and challenged at 21 days of age). The inoculum was produced from a Brazilian field strain of SH. At the end of the experiment, caecum and liver/ spleen samples were collected for quantitative and qualitative analysis of SH, respectively. Analysis of the liver/spleen showed that Poulvac® ST significantly (P ≤ 0.05) reduced the percentage of SH positivity in the group challenged at 3 days of age, while in the group challenged at 21 days this difference was almost considered significant (P = 0.1818). On the other hand, there was no statistically significant difference in SH count in the caecum (CFU/g) in the group challenged at 3 days, but for the group challenged at 21 days the SH counts were significantly (P ≤ 0.05) lower in the vaccinated group when compared to the positive control.ARTICLE HISTORY

show abstract

“…In developing countries, FT can cause poultry industries to suffer significant economic losses (Van Immerseel et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of safety and efficacy of a live Salmonella gallinarum vaccine candidate secreting an adjuvant protein with SG9R in chickens

Nandre

Lee

2014

Veterinary Immunology and Immunopathology

View full text Add to dashboard Cite

Salmonella Gallinarum field isolates from laying hens are related to the vaccine strain SG9R

Cited by 41 publications

References 35 publications

ERIC-PCR genotyping of field isolates ofSalmonella entericasubsp.entericaserovar Gallinarum biovars Gallinarum and Pullorum

ERIC-PCR genotyping of field isolates ofSalmonella entericasubsp.entericaserovar Gallinarum biovars Gallinarum and Pullorum

Evaluation of the effectiveness and safety of a genetically modified live vaccine in broilers challenged with Salmonella Heidelberg

Comparative evaluation of safety and efficacy of a live Salmonella gallinarum vaccine candidate secreting an adjuvant protein with SG9R in chickens

Contact Info

Product

Resources

About