During a three-year study (1969–1971) samples of feces from turtles imported from Yugoslavia and Greece as well as from Russia were examined for the presence of Salmonella. The species examined were Testudo hermanni, Testudo graeca and Testudo horsfieldii. From the 182 specimens examined, 131 (71.97%) proved to be Salmonella positive. 38 different serotypes were isolated. 107 specimens contained single serotypes, while 23 were doubly infected and 1 specimen harbored 3 different serotypes of Salmonella. 4 of the isolated serotypes belonged to the group of 47 most frequently occurring serotypes. From the 1971 shipment of turtles we were able to isolate a new serotype, S. gallen (11:a:1,2). Attempts to eliminate fecal excretion of Salmonellae by infected turtles were made by treatment with an antibiotic, Penbritin.