Salmonellae in food stuffs of plant origin and their implications on human health

Krtinić, G.; Đurić, Predrag; Ilić, Svetlana

doi:10.1007/s10096-010-1001-4

Cited by 17 publications

(13 citation statements)

References 37 publications

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“…For the produce results, the fact that cantaloupes and tomatoes had some of the highest Salmonella-positive samples is consistent with studies reporting their high Salmonella prevalence and their association with foodborne outbreaks (1,2,9,11). No Salmonella was isolated from Honduran produce for serotyping.…”

Section: Discussionsupporting

confidence: 85%

Salmonella in Beef and Produce from Honduras

Maradiaga

Miller

Thompson

et al. 2015

Journal of Food Protection

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Salmonella continues to cause a considerable number of foodborne illnesses worldwide. The sources of outbreaks include contaminated meat and produce. The purpose of this study was to establish an initial investigation of the burden of Salmonella in produce and beef from Honduras by sampling retail markets and abattoirs. Retail produce samples (cantaloupes, cilantro, cucumbers, leafy greens, peppers, and tomatoes; n = 573) were purchased in three major cities of Honduras, and retail whole-muscle beef (n = 555) samples were also purchased in four major cities. Additionally, both hide and beef carcass (n = 141) samples were collected from two Honduran abattoirs. Whole-muscle beef samples were obtained using a sponge hydrated with buffered peptone water, and 10 ml of the buffered peptone water rinsate of each produce sample was collected with a dry sponge and placed in a bag to be transported back to the United States. Salmonella was detected using a commercially available, closeplatform PCR system, and positive samples were subjected to culture on selective media to obtain isolates. Overall, the prevalence of Salmonella-positive samples, based on PCR detection in Honduras (n = 555) retail beef was 10.1% (95% confidence interval = 7.8, 12.9), whereas 7.8% (n = 141) of beef carcass and hides samples were positive in both beef plants. The overall Salmonella prevalence for all produce samples (n = 573) collected was 2.1% (95% confidence interval = 1.2, 3.6). The most common serotypes identified in Honduras were Salmonella Typhimurium followed by Derby. These results provide an indication of Salmonella contamination of beef and produce in Honduras. Developing a Salmonella baseline for Latin America through an initial investigation like the one presented here contributes to a broader global understanding of the potential exposure through food, thus providing insight into the needs for control strategies.

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Section: Discussionsupporting

confidence: 85%

Salmonella in Beef and Produce from Honduras

Maradiaga

Miller

Thompson

et al. 2015

Journal of Food Protection

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“…There have been a number of Salmonella outbreaks which have been traced to contaminated low-moisture foods, including milk powders, peanut butter, paprika-powdered potato chips, herbal teas and chocolate (Marth et al 1969 ;Sheth et al 2011 ;Sotir et al 2009 ). Although most cases of salmonellosis are traced back to eggs, meat consumption and other food stuffs of animal origin, an increasing number of outbreaks are due to Salmonella contamination of plant-originated food stuffs (Krtinić et al 2010 ). The organism can be found on the surface of plants, as the result of secondary contamination, but they can also occur in soil that is treated with a natural fertiliser, i.e.…”

Section: Salmonella Serovarsmentioning

confidence: 99%

Powdered Infant Formula

Forsythe

2014

The Microbiological Safety of Low Water Activity Foods and Spices

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"Powdered infant formula" (PIF) is a term used to cover both breast milk fortifi ers and breast milk substitutes. Both are given to neonates who are immunedefi cient. Although their low water activity stops bacterial growth during storage, bacteria are able to grow rapidly following reconstitution. Such organisms may be present through intrinsic contamination or through extrinsic contamination from the water, preparation equipment or carer. This chapter focuses on Cronobacter spp. and Salmonella serovars due the substantiated cases of neonatal infections through the ingestion of contaminated PIF, as emphasized by the three FAO-WHO risk assessments on the microbiological safety of PIF.The taxonomic re-evaluations of the Cronobacter genus are presented, emphasizing the improved understanding using Next Generation Sequencing, along with multilocus sequence typing to reveal clonal lineages. One particular lineage, C. sakazakii clonal complex 4, is particularly associated with neonatal meningitis cases. It is also one of the dominant sequence types that has been isolated from PIF manufacturing plants and PIF. The organism can survive spray-drying and persist for long periods in the dried state. Salmonella is another organism of concern for neonatal infections, and although better understood than Cronobacter there have been rare outbreaks due to atypical, lactose-fermenting strains.

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“…Hanning et al (2009) summed up S. enterica outbreaks in the USA based on the consumption of fresh produce showing that many different vegetable plant species were involved in those outbreaks. Due to its importance as produce contaminant much research has been done in this direction proving the high potential of S. enterica to colonize surfaces as well as interior of various plants (Brandl, 2006; Berger et al, 2010; Krtinić et al, 2010). Listeria monocytogenes outbreaks based on the consumption of fresh produce were reported in lower numbers compared to those derived from other food sources (US Food and Drug Administration, 2003).…”

Section: Introductionmentioning

confidence: 99%

Colonization of plants by human pathogenic bacteria in the course of organic vegetable production

et al. 2014

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In recent years, increasing numbers of outbreaks caused by the consumption of vegetables contaminated with human pathogenic bacteria were reported. The application of organic fertilizers during vegetable production is one of the possible reasons for contamination with those pathogens. In this study laboratory experiments in axenic and soil systems following common practices in organic farming were conducted to identify the minimal dose needed for bacterial colonization of plants and to identify possible factors like bacterial species or serovariation, plant species or organic fertilizer types used, influencing the success of plant colonization by human pathogenic bacteria. Spinach and corn salad were chosen as model plants and were inoculated with different concentrations of Salmonella enterica sv. Weltevreden, Listeria monocytogenes sv. 4b and EGD-E sv. 1/2a either directly (axenic system) or via agricultural soil amended with spiked organic fertilizers (soil system). In addition to PCR- and culture-based detection methods, fluorescence in situ hybridization (FISH) was applied in order to localize bacteria on or in plant tissues. Our results demonstrate that shoots were colonized by the pathogenic bacteria at inoculation doses as low as 4 × 10 CFU/ml in the axenic system or 4 × 105 CFU/g in the soil system. In addition, plant species dependent effects were observed. Spinach was colonized more often and at lower inoculation doses compared to corn salad. Differential colonization sites on roots, depending on the plant species could be detected using FISH-CLSM analysis. Furthermore, the transfer of pathogenic bacteria to plants via organic fertilizers was observed more often and at lower initial inoculation doses when fertilization was performed with inoculated slurry compared to inoculated manure. Finally, it could be shown that by introducing a simple washing step, the bacterial contamination was reduced in most cases or even was removed completely in some cases.

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Salmonellae in food stuffs of plant origin and their implications on human health

Cited by 17 publications

References 37 publications

Salmonella in Beef and Produce from Honduras

Salmonella in Beef and Produce from Honduras

Powdered Infant Formula

Colonization of plants by human pathogenic bacteria in the course of organic vegetable production

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