Salt secretion, proline accumulation and increased branching confer tolerance to drought and salinity in the endemic halophyte Limonium linifolium

Tabot, Pascal Tabi; Adams, Janine B.

doi:10.1016/j.sajb.2014.05.009

Cited by 23 publications

(18 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To our knowledge, this is the first systematic study of this kind in Limonium . Most previously published reports using similar approaches had a much more limited scope, focusing on a single Limonium species at a particular developmental stage, and generally characterizing a narrower range of salt-induced responses (Zia and Khan, 2004 ; Tabot and Adams, 2014 ; Souid et al, 2016 ). Moreover, there are very few studies focused on comparative analyses of stress responses in congener species with different scales of geographic distribution; for example, those carried out in Artemisia (Ishikawa and Kachi, 2000 ) or Gypsophila (Soriano et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…The five leaves per harvested plant previously selected to determine LA were used to recover ions on the leaf surface, by immersing each leaf in 15 ml MilliQ water for 10 min; dissolved ions were directly measured in the solution and their contents were expressed as μmol per cm 2 of LA. This simple method, based on Tabot and Adams ( 2014 ), allows quantification of the total amount of salt accumulated on the surface of the leaves, although not the secretion rate of salt glands; yet it is useful for comparative analyses of related species subjected to the same salt treatments, as those described in this paper.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Unraveling Salt Tolerance Mechanisms in Halophytes: A Comparative Study on Four Mediterranean Limonium Species with Different Geographic Distribution Patterns

et al. 2017

View full text Add to dashboard Cite

We have performed an extensive study on the responses to salt stress in four related Limonium halophytes with different geographic distribution patterns, during seed germination and early vegetative growth. The aims of the work were twofold: to establish the basis for the different chorology of these species, and to identify relevant mechanisms of salt tolerance dependent on the control of ion transport and osmolyte accumulation. Seeds were germinated in vitro, in the presence of increasing NaCl concentrations, and subjected to “recovery of germination” tests; germination percentages and velocity were determined to establish the relative tolerance and competitiveness of the four Limonium taxa. Salt treatments were also applied to young plants, by 1-month irrigation with NaCl up to 800 mM; then, growth parameters, levels of monovalent and divalent ions (in roots and leaves), and leaf contents of photosynthetic pigments and common osmolytes were determined in control and stressed plants of the four species. Seed germination is the most salt-sensitive developmental phase in Limonium. The different germination behavior of the investigated species appears to be responsible for their geographical range size: L. narbonense and L. virgatum, widespread throughout the Mediterranean, are the most tolerant and the most competitive at higher soil salinities; the endemic L. santapolense and L. girardianum are the most sensitive and more competitive only at lower salinities. During early vegetative growth, all taxa showed a strong tolerance to salt stress, although slightly higher in L. virgatum and L. santapolense. Salt tolerance is based on the efficient transport of Na+ and Cl− to the leaves and on the accumulation of fructose and proline for osmotic adjustment. Despite some species-specific quantitative differences, the accumulation patterns of the different ions were similar in all species, not explaining differences in tolerance, except for the apparent activation of K+ transport to the leaves at high external salinity, observed only in the most tolerant L. narbonense and L. virgatum. This specific response may be therefore relevant for salt tolerance in Limonium. The ecological implications of these results, which can contribute to a more efficient management of salt marshes conservation/regeneration programs, are also discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Unraveling Salt Tolerance Mechanisms in Halophytes: A Comparative Study on Four Mediterranean Limonium Species with Different Geographic Distribution Patterns

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The P5CS1 gene encodes a key component in proline biosynthesis and accumulation. Many studies have highlighted the positive correlation of proline accumulation with adaptability of plants in response to several stresses such as drought and salt stress [69][70][71]. Greatly higher expression of P5CS1, especially in roots, has been detected in the population of M. arborea compared to that of M. sativa and Alborea.…”

Section: Drought Stress and Transcriptional Responsesmentioning

confidence: 99%

Growth, Physiological, Biochemical, and Transcriptional Responses to Drought Stress in Seedlings of Medicago sativa L., Medicago arborea L. and Their Hybrid (Alborea)

et al. 2019

View full text Add to dashboard Cite

Medicago sativa L. is a tetraploid perennial forage legume of great agronomical interest. The increasing need for its use under water-deficit conditions as well as low-input systems demands further improvement of its drought tolerance. On the other hand, Medicagoarborea L. is a perennial leguminous shrub, which is knownas a drought-tolerant species. In the present study, drought stress responses of the aforementioned medicago species, along with their hybrid, named Alborea, were comparatively assayed at the morphological, physiological, biochemical, and transcriptional levels. In particular, transcript abundance of representative genes that: (a) control ion transport, intracellular Na + /H + antiporters(NHX1) and rare cold inducible2A (RCI2A); (b) have an osmotic function ∆1-pyrroline-5-carboxylate synthetase 1 (P5CS1); and (c) participate in signaling pathways and control cell growth and leaf function stress-induced mitogen-activated protein kinases kinases (SIMKK), Zinc Finger (ZFN), apetala2/ethylene-responsive element binding (AP2/EREB), basic leucine zipper (bzip) and Medicago sativa Helicase 1(MH1) were evaluated. Under well-watered conditions, the studied population of Alborea showed the highest stem elongation rate and photosynthetic rate that were dramatically reduced under drought conditions compared to M. sativa and M. arborea. Under drought conditions, the studied population of M. arborea showed less reduction of relative water content, all gas-exchange parameters, less lipid peroxidation, and more antioxidant capacity. Moreover, transcriptional analysis demonstrated that the population of M. arborea exhibited significantly higher transcript levels of drought-responsive genes in both leaves and roots under drought stress conditions. M. sativa has better antioxidant capacity than Alborea and had a higher induction of stress-related genes, thus it performs better than Alborea under drought conditions. Among the studied genes, it seems that AP2/EREB play a critical role in the response of the studied population to drought stress.

show abstract

“…Plants show a range of morphological and physiological adaptations to cope with these factors (Naidoo et al, 1992;Colmer and Flowers, 2008;Flowers and Colmer, 2008). For instance, plants can produce glands for salt extrusion in high salinity environments (Tabot and Adams, 2014) and aerenchyma and adventitious roots to allow oxygen transport to the root tips in sediment with low redox (Armstrong, 2000;Nishiuchi et al, 2012). Furthermore, the low nutrient status of sandy soils and their mobility could also affect root development (Olff et al, 1997;Tyler and Zieman, 1999;Schutten et al, 2005;Fourcaud et al, 2008;.…”

Section: Introductionmentioning

confidence: 99%

Intraspecific Root Trait Variability Along Environmental Gradients Affects Salt Marsh Resistance to Lateral Erosion

et al. 2019

View full text Add to dashboard Cite

Recent studies in salt marshes have demonstrated the role of plant roots in sediment stabilisation, and hence the importance of marshes in providing coastal protection. However, the relative role of root traits and environmental factors in controlling sediment stability, and how intraspecific variability of root traits vary within and among marshes, remain poorly understood. In this study, we investigated which root trait(s) drive sediment stability (resistance to lateral erosion) in two marsh species with an important role in coastal protection (Spartina anglica and Atriplex portulacoides) and how the environment affects the expression of these traits. We sampled three marshes along salinity gradients in each of two estuaries in Wales (UK), establishing replicate plots in the respective dominant zones of each species. In all plots we sampled abiotic variables (sand, redox potential, pH, salinity) and root traits (root density, specific root density, root volume, root length density); in a subset of these plots (three per species in each marsh) we extracted soil-plant cores and assessed their erosion resistance in a flume. Sediment stability was enhanced by increases in root density and reductions in sand content. Abiotic variables affected root density in different ways depending on species: in S. anglica, redox was the only significant factor, with a positive, linear effect on root density; in A. portulacoides, redox had a non-linear (U-shaped) effect on root density, while sand had a negative effect. Collectively, these results show that (i) intraspecific variability in root density can influence sediment stability in salt marshes, and (ii) sediment properties not only influence sediment stability directly, but also indirectly via root density. These results shed light on spatial variability in the stability of salt marshes to lateral erosion and suggest that root density should be incorporated into coastal vegetation monitoring programs as an easy-to-measure root trait that links the environment to sediment stability and hence to the function and services provided by marshes.

show abstract

Salt secretion, proline accumulation and increased branching confer tolerance to drought and salinity in the endemic halophyte Limonium linifolium

Cited by 23 publications

References 37 publications

Unraveling Salt Tolerance Mechanisms in Halophytes: A Comparative Study on Four Mediterranean Limonium Species with Different Geographic Distribution Patterns

Unraveling Salt Tolerance Mechanisms in Halophytes: A Comparative Study on Four Mediterranean Limonium Species with Different Geographic Distribution Patterns

Growth, Physiological, Biochemical, and Transcriptional Responses to Drought Stress in Seedlings of Medicago sativa L., Medicago arborea L. and Their Hybrid (Alborea)

Intraspecific Root Trait Variability Along Environmental Gradients Affects Salt Marsh Resistance to Lateral Erosion

Contact Info

Product

Resources

About