Salvia Fruticosa-Mediated Antioxidant Protection Against Oxidative Stress in Streptozotocin – Induced Diabetic Rats Role of Α-Glucosidase Activity

Hamza, Alaaeldin Ahmed; Ahmed, Samir; Elwy, Hanan Mohamed; Radwan, Omyma K.

doi:10.21276/ijpbs.2016.6.3.16

Cited by 2 publications

(1 citation statement)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding non-volatiles, about 160 polyphenolic compounds were identified from sage plants: flavonoids and their glycosides, anthocyanins, and phenolic acids with characteristic caffeic acid derivatives, such as rosmarinic acid, phenolic diterpenes, such as carnosic acid, and other phenolic glycosides [ 7 , 8 ]. The latter play an important role in the prevention against oxidative damages [ 5 , 6 , 7 , 8 , 9 ]. It is very important to draw to the attention that, in the case of thujone, notwithstanding many beneficial effects, the tremendously neurotoxic danger of this volatile organic constituent has been checked out [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Chemical Characterization and Antioxidant, Antibacterial, Antiacetylcholinesterase and Antiproliferation Properties of Salvia fruticosa Miller Extracts

et al. 2023

View full text Add to dashboard Cite

The Salvia fruticosa (Mill.) is the most medicinal plant used in Lebanon. The aim of this study is to investigate the phytochemical composition and the biological activities (in vitro) of its extracts. The plant was extracted by cold maceration with four solvents presenting an increasing polarity: cyclohexane (CHX), dichloromethane (DCM), ethyl acetate (EtOAc) and methanol (MeOH). The extracts were screened for their chemical composition by a HPLC-DAD detector for phenolic compounds identification and quantification and by GC-MS for volatile compounds detection. The antioxidant capacity (DPPH inhibition) was tested. Biological activities, mainly anti-Alzheimer activity (acetylcholinesterase inhibition), the antiproliferation of two human colon cancer cell lines (HCT-116 and Caco-2 cells) and antibacterial activity, were evaluated. Ten aromatic compounds were quantified by HPLC-DAD analysis. A total of 123 compounds were detected by GC-MS analysis. The MeOH extract showed a very interesting antioxidant activity with an inhibition percentage (IP) of 76.1% and an IC50 of 19.4 μg/mL. The EtOAc extract exhibited the strongest inhibition against the acetylcholinesterase activity (IP = 60.6%) at 50 μg/mL. It also strongly inhibited the proliferation of the HCT-116 cells (IP = 87.5%), whereas the DCM extract gave the best result with the Caco-2 cells (IP = 72.3%). The best antibacterial activity was obtained with the MeOH extract against Staphylococcus aureus (MIC = 1.2 μg/mL) and with the EtOAc extract against Escherichia coli (MIC = 2.4 μg/mL). This study highlights the chemical composition and therapeutic potential of S. fruticosa. It is important to mention that the following chemical compounds were identified for the first time in plant extracts: 2,6,11,15-tetramethyl-hexadeca-2,6,8,10,14-pentaene; 4,5,6,7-tetrahydroxy-1,8,8,9-tetramethyl-8,9-dihydrophenaleno [1,2-b]furan-3-one; podocarpa-1,8,11,13-tetraen-3-one,14-isopropyl-1,13-dimethoxy; podocarpa-8,11,13-trien-3-one,12-hydroxy-13-isopropyl-,acetate; 3′,8,8′-trimethoxy-3-piperidin-1-yl-2,2′-binaphthyl-1,1′,4,4′-tetrone; and 2,3-dehydroferruginol, thus underlining the originality of this study.

show abstract