Salvianolic acid B inhibits mitochondrial dysfunction by up-regulating mortalin

Abstract

Salvianolic acid B is an antioxidative ingredient derived from Radix Salviae miltiorrhizae that has been widely used to treat liver diseases. However, the therapeutic mechanism underlying Salvianolic acid B has remained largely unknown. Our studies verified that Salvianolic acid B efficiently blocked mitochondrial deformation and dysfunction induced by H2O2 in the human hepatocyte cell line HL7702. Mortalin, a mitochondrial molecular chaperone, maintains mitochondrial morphology stabilization and function integrity. Previous results showed that mortalin overexpression has been observed in hematoma carcinoma cells and that mortalin maintains mitochondrial homeostasis and antagonizes oxidative stress damage. We found that Salvianolic acid B significantly up-regulated mortalin protein expression levels. In addition, Salvianolic acid B lost the function of preventing mitochondrial deformation and dysfunction induced by oxidative stress under mortalin knockdown conditions. We further found that mortalin overexpression increases the mRNA expression of mitofusin-related factor Mfn1 and mitofission-related factor hFis1. In conclusion, Salvianolic acid B maintains the mitochondrial structure stabilization and functional integrity by up-regulating mortalin, which may be associated with increased mitofusin factor Mfn1 and reduced mitofission factor hFis1.

Salvianolic acid B protects hepatocytes from H2O2injury by stabilizing the lysosomal membrane

“…Sal B is a major water-soluble polyphenolic antioxidant in Radix Salviae Miltiorrhizae and has been extensively used in clinics in China[ 23 - 25 ]. Sal B possesses efficient radical scavengers and antioxidants[ 14 , 26 , 27 ]. Previous reports have shown that Sal B exerts therapeutic effects in treating liver fibrosis[ 28 , 29 ].…”

Salvianolic Acid B Improves Mitochondrial Function in 3T3-L1 Adipocytes Through a Pathway Involving PPARγ Coactivator-1α (PGC-1α)

“…Furthermore, it has been shown to moderate lipid metabolism in high-fat diet-induced obese mice by suppressing peroxisome proliferator activated receptor gamma (PPARγ)- mediated adipogenesis ( Wang et al, 2014 ). SalB has also been found to improve the integrity of mitochondria and block mitochondria deformation and dysfunction induced by H 2 O 2 ( Xu et al, 2011 ; Liu et al, 2017 ). However, the effects of SalB on mitochondrial function in 3T3-L1 cells remain largely unknown.…”

Salvianolic Acids: Potential Source of Natural Drugs for the Treatment of Fibrosis Disease and Cancer

“…Sal B inhibited the proliferation of LX-2 cells and down-regulated the expression of α-SMA protein (Hou et al, 2011). The study also found that Sal B protected the liver from damage by effectively blocking H 2 O 2 -induced mitochondrial deformation and dysfunction in the human liver cell line HL7702 (Liu et al, 2017).…”